Spatial, temporal, and sexually dimorphic expression patterns of thefruitless gene in theDrosophila central nervous system

Lee, Gyunghee; Foss, Margit; Goodwin, Stephen F.; Carlo, Troy; Taylor, Barbara J.; Hall, Jeffrey C.

doi:10.1002/1097-4695(20000615)43:4<404::aid-neu8>3.0.co;2-d

Cited by 216 publications

(275 citation statements)

References 36 publications

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“…Neurogenetic findings pertinent to this matter are that C309 is expressed in imaginal thoracic ganglia (Fig. 3 and Table 2); fru is ''songinvolved'' (12,37); this gene makes its products within several regions of the ventral nerve cord (16,17,(32)(33)(34), coexpressing C309 within most of them (Table 2); and genetic maleness within Drosophila's VNC has been implicated in song control (reviewed in ref. 35).…”

Section: Discussionmentioning

confidence: 99%

“…3). We compared C309 and FRU M expression patterns during metamorphosis, shortly after fru's sex promoter is initially activated (16). Examination of CNS specimens dissected from C309͞UAS-egfp pupae revealed overlap between GFP and FRU M signals in the same 10 clusters observed for adults, although the coexpressing patterns within pupae were not as extensive (Fig.…”

Section: Cns Expression Of Fruitless and A Transgene Markermentioning

confidence: 93%

“…The secondary antibody applied to recognize anti-FRU M primary (see ref. 16), was Cy5-conjugated anti-rat IgG. Neuronal marking was analyzed by using a Leica TCS SP2, scanning in the Cy5 channel for singly labeled brains (anti-FRU M ) or scanning for Cy5 (red) and GFP (green) in colocalization assessments, which included conversion of red to magenta and merging the two channels to create white readout.…”

Section: Stocks Of D Melanogaster Crosses and Fly Handlingsmentioning

confidence: 99%

“…16) within the brain or ventral nerve cord (VNC). The fourth column includes novel counts of adult neurons, determined by application of anti-FRU M to CNSs of wild-type (WT) males (average numbers of immunoreactive cells per hemi-CNS region, Ϯ SEM; n, number of specimens scored).…”

Section: Fru-abmentioning

confidence: 99%

“…12 and 13). The original fruitless mutation leads to spatially nonrandom decreases of fru-product presence (14,15) within particular subsets of the normal CNS expression pattern (16,17), which may be causally connected with the breakdown of recognition that is a salient effect of fru 1 on male behavior (9,12).…”

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confidence: 99%

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Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship

Villella

Ferri²,

Krystal³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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A gal4-containing enhancer-trap called C309 was previously shown to cause subnormal courtship of Drosophila males toward females and courtship among males when driving a conditional disrupter of synaptic transmission (shi TS ). We extended these manipulations to analyze all features of male-specific behavior, including courtship song, which was almost eliminated by driving shi TS at high temperature. In the context of singing defects and homosexual courtship affected by mutations in the fru gene, a tra-regulated component of the sex-determination hierarchy, we found a C309͞tra F combination also to induce high levels of courtship between pairs of males and ''chaining'' behavior in groups; however, these doubly transgenic males sang normally. Because production of male-specific FRU M protein is regulated by TRA, we hypothesized that a fru-derived transgene encoding the male (M) form of an Inhibitory RNA (fru MIR ) would mimic the effects of tra F ; but C309͞fru MIR males exhibited no courtship chaining, although they courted other males in single-pair tests. Doublelabeling of neurons in which GFP was driven by C309 revealed that 10 of the 20 CNS clusters containing FRU M in wild-type males included coexpressing neurons. Histological analysis of the developing CNS could not rationalize the absence of tra F or fru MIR effects on courtship song, because we found C309 to be coexpressed with FRU M within the same 10 neuronal clusters in pupae. Thus, we hypothesize that elimination of singing behavior by the C309͞shi TS combination involves neurons acting downstream of FRU M cells reproductive behavior ͉ C309 enhancer trap ͉ shi TS transgene ͉ tra F transgene ͉ inhibitory fru RNA transgene

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Section: Discussionmentioning

confidence: 99%

Section: Cns Expression Of Fruitless and A Transgene Markermentioning

confidence: 93%

Section: Stocks Of D Melanogaster Crosses and Fly Handlingsmentioning

confidence: 99%

Section: Fru-abmentioning

confidence: 99%

mentioning

confidence: 99%

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Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship

Villella

Ferri²,

Krystal³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Characterization of Drosophila fruitless‐gal4 transgenes reveals expression in male‐specific fruitless neurons and innervation of male reproductive structures

Billeter

Goodwin

2004

J of Comparative Neurology

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The fruitless (fru) gene acts in the central nervous system (CNS) of Drosophila melanogaster to establish male sexual behavior. Genetic dissection of the locus has shown that one of the fru gene's promoter, P1, controls the spatial and temporal expression of male-specific FruM proteins critical to determining stereotypical male sexual behavior. By using the Gal4-expression system, we show that a 16-kb fragment of the fru P1 promoter's 5' regulatory region drives the expression of Gal4 in a subset of FruM-expressing neurons within both the pupal and adult CNS. Colocalization of FruM and a Gal4-responsive reporter shows that the fru(P1)-gal4 fusion construct generates expression in both previously characterized FruM-expressing neurons as well as within cells of both the CNS and the peripheral nervous system that have not been demonstrated as FruM-expressing. Gal4-expressing neurons are shown to innervate abdominal organs directly relevant to fru function; specifically, the muscle of Lawrence (MOL) and the male internal reproductive organs. Innervations of the latter are shown to originate from identified FruM-serotonergic neurons. Furthermore, we show that the MOL neuromuscular junction is sexually dimorphic. Finally, we describe Gal4 expression in neurites innervating male reproductive structures that are hypothesized to be targets of fru function. Isolation of the regulatory sequences controlling the expression of fru in the CNS, therefore, provides a potent tool for the manipulation of FruM-expressing neurons and for understanding the cellular basis of Drosophila reproductive behavior.

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Comparative analysis ofCorazonin‐encoding genes (Crz's) inDrosophilaspecies and functional insights intoCrz‐expressing neurons

Choi

Lee

Hall

et al. 2005

J of Comparative Neurology

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108

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To gain insight into regulatory mechanisms of tissue-specific Corazonin (Crz) gene expression and its functions in Drosophila, we cloned the Crz genes from four Drosophila species (D. melanogaster, D. simulans, D. erecta, and D. virilis) and performed comparative analyses of Crz gene sequences and expression patterns using in situ hybridization and immunohistochemistry. Although Crz gene sequences showed a great deal of diversity, its expression patterns in the CNS were highly conserved in the Drosophila species examined here. In D. melanogaster larva, Crz expression was found in four pairs of neurons per cerebral lobe and in eight pairs of bilateral neurons in the ventral nerve cord; in adult, the number of Crz-producing neurons increased to 6-8 in the pars lateralis of each brain lobe, whereas neurons in the ventral nerve cord were no longer detectable. Crz transcripts were also found in the optic lobes; however, these mRNAs do not seem to be translated. Such adult-like Crz expression patterns were established within 48 hours after pupation. Somata of Crz-neurons in the pars lateralis are located in the vicinity of terminals emanating from PDF-containing pacemaking neurons, indicating a functional connection between the two peptidergic nervous systems. A subset of Crz neurons coexpressed the period clock gene; however, normal Crz transcription was unaffected by central clockworks. Two pairs of ectopic Crz cells were detected in the adult brains of behaviorally arrhythmic Clock(Jrk) or cycle(02) mutants, suggesting that CLOCK and CYCLE proteins negatively regulate Crz transcription in a cell-specific manner.

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Spatial, temporal, and sexually dimorphic expression patterns of thefruitless gene in theDrosophila central nervous system

Cited by 216 publications

References 36 publications

Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship

Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship

Characterization of Drosophila fruitless‐gal4 transgenes reveals expression in male‐specific fruitless neurons and innervation of male reproductive structures

Comparative analysis ofCorazonin‐encoding genes (Crz's) inDrosophilaspecies and functional insights intoCrz‐expressing neurons

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