Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment

Abramovich, Dalhia; Celin, Alejandra Rodriguez; Hernandez, Fátima; Tesone, Marta; Parborell, Fernanda

doi:10.1530/rep-08-0130

Cited by 39 publications

(33 citation statements)

References 43 publications

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“…It is worthy to investigate other than vascular (extravascular) actions of VEGF in the growing follicle, to provide information about VEGF as a possible marker for high-quality follicles destined to provide a mature oocyte capable of fertilization. Except for VEGF, the other important angiogenic factor in ovarian folliculogenesis, suggested from the studies in rodent [25], bovine [26], and monkey [27], is ANGPT-1 since its mRNA and protein expression increase in antral follicles. The high ANGPT-2:ANGPT-1 ratio in developing follicles in vitro was found in a granulosa cell study, where nonluteinized cells produced more ANGPT-2 than ANGPT-1 during a short-term culture.…”

Section: Angiogenesis and Ovarian Functionmentioning

confidence: 99%

Molecular regulation and role of angiogenesis in reproduction

Rizov¹,

Andreeva

Dimova

2017

Taiwanese Journal of Obstetrics and Gynecology

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Angiogenesis is an essential process for proper functioning of the female reproductive system and for successful pregnancy realization. The multitude of factors required for physiological angiogenesis and the complexity of regulation of their temporal-spatial activities contribute to aberrations in human fertilization and pregnancy outcomes. In this study, we reviewed the current knowledge of the temporal expression patterns, functions, and regulatory mechanisms of angiogenic factors during foliculogenesis, early implantation/placentation and embryo development, as well as recurrent spontaneous abortions. Angiogenic factors including vascular endothelial growth factors and angiopoietins have documented roles in the development of primordial follicles into mature antral follicles. They also participate in decidualization, which is accompanied by the creation of an extensive network of vessels in the stromal bed that support the growth of the embryo and the placenta, and maintain early pregnancy. During placentation angiogenic and angiomodulatory cytokines, T and B lymphocytes and macrophages affect angiogenesis in a context-dependent manner. Defects in angiogenesis at the maternal-fetal interface contribute to miscarriage in humans. The establishment of more polymorphisms in the genes involved in angiogenesis/vasculogenesis, and their pathological phenotype and expression could give opportunities for prediction, creating a therapeutic strategy, and treatment of diseases related to female reproductive health and problematic conception.

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Section: Angiogenesis and Ovarian Functionmentioning

confidence: 99%

Molecular regulation and role of angiogenesis in reproduction

Rizov¹,

Andreeva

Dimova

2017

Taiwanese Journal of Obstetrics and Gynecology

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“…In both cases, individual ovarian follicles were dissected from the ovary under a stereoscopic microscope using fine needles, as described previously (Parborell et al 2002, Abramovich et al 2009). …”

Section: Follicle Isolationmentioning

confidence: 99%

Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat

et al. 2013

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Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development.

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“…Expression of ANGPT1 and ANGPT2 has been observed in cow, mouse, and rat theca interna and in human and rhesus monkey granulosa cells in the ovary (18)(19)(20)(21)(22)(23)(24). However, the role of ANGPTs in human FF during the preovulatory phase remains poorly understood.…”

mentioning

confidence: 99%