Special Selectivity of Molecularly Imprinted Monolithic Stationary Phase

Abstract

A monolithic, molecularly imprinted column was prepared by an in situ therm-initiated copolymerization process; the essential conditions of forming a noncovalent molecular imprinting stationary phase are discussed and illustrated with caffeine and theophylline as examples from the angle of molecular construction, and examined by experiments. The results showed that the theophylline-imprinted monolithic column has high selectivity to theophylline; baseline separation of caffeine and theophylline was achieved. However, only weak specific selectivity to the template molecule was observed for the caffeine imprinted monolithic column.

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

“…Separation of the corresponding enantiomers was achieved but the separation mechanism was not mentioned. In recent years, the uses of monolithic media for superior chromatographic separation in high-performance liquid chromatography and capillary electrochromatography have attracted considerable attention [94][95][96][97][98][99][100][101][102].…”

Preparation of Molecularly Imprinted Polymer for Extracting Flavones fromChamaecyparis Obtusa

“…Molecular imprinting is a technique to create template-shaped cavities in polymer matrices with a memory of the template molecules to be used in molecular recognition (Yan, Jin, and Row 2005;Cormack and Elorza 2004). Molecularly imprinted polymers (MIPs) possess several advantages over their biological counterparts, including low cost, ease of preparation, storage stability, high mechanical strength, and applicability in harsh chemical media (Mahony et al 2005;Joshi, Kulkarni, and Mashelkar 2000).…”

Novel molecularly imprinted monolithic column for selective on‐line extraction of ciprofloxacin from human urine

“…It was prepared by one-step, in-situ polymerization 'molding' process directly within a chromatographic column without the tedious procedures of grinding, sieving and column packing. Compared with conventional particle columns, the monolithic columns possess a unique porous structure, and large through-pores exist in these monoliths, which enable mobile phases to flow through the adsorbents with low flow resistance at high flow rates (Li et al, 2005;Huang et al, 2004;Yan et al, 2005). As a technique for the creation of artificial receptor-like binding sites with a 'memory' for the shape and functional group positions of the template molecule, MIP has drawn much attention and has been used successfully in many fields of chemistry and biology, including chromatography (Hosoya et al, 1998), artificial antibodies (Ye and Mosbach, 2001), chemical sensors (Marx et al, 2004) and solid-phase extraction (Masquée et al, 2001;Jodlbauer et al, 2002;Qiao et al, 2006).…”