Specialized Histology for Neuropathology

Hofecker, Jennifer L.; Moss, Tracie L.; Bêcher, Mark W.

doi:10.1179/his.2007.30.2.67

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Because of their simplicity and operational expedience, colorimetric and fluorescence/luminescence staining are the basis of broadly used analytical techniques for biomolecules, − bacterial species, − eukaryotic cells, − and tissue samples. − Because of its inherent sensitivity, fluorescence staining is a central component of bioanalytical assays and imaging methodologies. − …”

Section: Introductionmentioning

confidence: 99%

Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

et al. 2010

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For more than a century, colorimetric and fluorescence staining have been the foundation of a broad range of key bioanalytical techniques. The dynamics of such staining processes, however, still remains largely unexplored. We investigated the kinetics of fluorescence staining of two gram-negative and two gram-positive species with 3,3'-diethylthiacyanine (THIA) iodide. An increase in the THIA fluorescence quantum yield, induced by the bacterial dye uptake, was the principal reason for the observed emission enhancement. The fluorescence quantum yield of THIA depended on the media viscosity and not on the media polarity, which suggested that the microenvironment of the dye molecules taken up by the cells was restrictive. The kinetics of fluorescence staining did not manifest a statistically significant dependence neither on the dye concentration, nor on the cell count. In the presence of surfactant additives, however, the fluorescence-enhancement kinetic patterns manifested species specificity with statistically significant discernibility.

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Section: Introductionmentioning

confidence: 99%

Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

et al. 2010

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“…(c) Neuropathology specimens have both high lipid content (due to myelin) and high-water content, and therefore require dedicated processing with increased dehydration, clearing, and infiltration times [10,32].…”

Section: Selecting a Tissue Processing Schedulementioning

confidence: 99%

Tissue Processing

Aziz

Zeman-Pocrnich

2021

Methods in Molecular Biology

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Tissue processing is the technique by which fixed tissues are made suitable for embedding within a supportive medium such as paraffin, and consists of three sequential steps: dehydration, clearing, and infiltration. In most clinical and research settings, tissue processing is accomplished using an automated tissue processor, with or without microwave-assistance. To ensure high-quality results, processing protocols should be tailored to tissue size and composition by modifying variables such as reagents used and the timing of the various steps. Herein, we provide an overview of tissue processing theory and outline a basic tissue processing method for use with a conventional automated fluid transfer/enclosed processor. The principles described will assist readers in optimizing tissue processing for their own projects.

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Grossing biopsies: an introduction to general principles and techniques

Dimenstein

2009

Annals of Diagnostic Pathology

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Specialized Histology for Neuropathology

Cited by 4 publications

References 18 publications

Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

Tissue Processing

Grossing biopsies: an introduction to general principles and techniques

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