Speciation of coagulase negative staphylococci, isolated from indoor air, using SDS page gel bands of expressed proteins followed by MALDI TOF MS and MALDI TOF-TOF MS-MS analysis of tryptic peptides

Fox, Kevin M.; Fox, Alvin; Rose, John R.; Walla, Michael D.

doi:10.1016/j.mimet.2010.12.007

Cited by 30 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, misidentification of S. saprophyticus by these simplified conventional and automated phenotypic methods often occurs. Although matrixassisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for many years in the fields of proteomics and toxicology, the technology has only recently been applied to the field of microbiology for species identification of bacteria (2,(10)(11)(12)(13)(14)(15)(16). Direct detection of urinary tract pathogens from urine samples with high accuracy by MALDI-TOF MS has been reported, especially for Gram-negative bacteria with high bacterial counts (17).…”

mentioning

confidence: 99%

Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

et al. 2013

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Once a protein has been identified, MS alone is all that is needed to identify a group of species (Fox et al, 2011, Kooken et al, 2012.…”

Section: Gel Separation Followed By Maldi-tof Ms and Maldi-tof-tof Msmentioning

confidence: 99%

“…With MALDI-TOF-TOF MS-MS, however, one can separate desired intact proteins (bottom-down approach) using techniques such as gel electrophoresis or liquid chromatography, then analyze higher molecular weight proteins (50−100kDa) by enzymatically digesting the proteins into their respective peptides (Fox et al, 2011). The results of MS-MS can enhance the protein profile information obtained in MS.…”

Section: Gel Separation Followed By Maldi-tof Ms and Maldi-tof-tof Msmentioning

confidence: 99%

“…It is also necessary to have some prior knowledge of the gene of interest in order to create operational primers. Additionally, effective primer selection requires there be two conserved regions for genetic recognition and amplification, thereby limiting the number of useful genes (Jabbour et al, 2010;Fox et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Only certainty above 85% is considered significant. Using this criterion, the API strip was only able to identify 3/8 of the staphylococcal isolates to the species level (Fox et al, 2010;Fox et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria

Intelicato-Young

Fox

2013

Journal of Microbiological Methods

Self Cite

View full text Add to dashboard Cite

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

Duriez¹,

Armengaud

Fenaille

et al. 2016

J. Mass Spectrom.

View full text Add to dashboard Cite

In the current context of international conflicts and localized terrorist actions, there is unfortunately a permanent threat of attacks with unconventional warfare agents. Among these, biological agents such as toxins, microorganisms, and viruses deserve particular attention owing to their ease of production and dissemination. Mass spectrometry (MS)-based techniques for the detection and quantification of biological agents have a decisive role to play for countermeasures in a scenario of biological attacks. The application of MS to every field of both organic and macromolecular species has in recent years been revolutionized by the development of soft ionization techniques (MALDI and ESI), and by the continuous development of MS technologies (high resolution, accurate mass HR/AM instruments, novel analyzers, hybrid configurations). New possibilities have emerged for exquisite specific and sensitive detection of biological warfare agents. MS-based strategies for clinical application can now address a wide range of analytical questions mainly including issues related to the complexity of biological samples and their available volume. Multiplexed toxin detection, discovery of new markers through omics approaches, and identification of untargeted microbiological or of novel molecular targets are examples of applications. In this paper, we will present these technological advances along with the novel perspectives offered by omics approaches to clinical detection and follow-up.

show abstract

Speciation of coagulase negative staphylococci, isolated from indoor air, using SDS page gel bands of expressed proteins followed by MALDI TOF MS and MALDI TOF-TOF MS-MS analysis of tryptic peptides

Cited by 30 publications

References 39 publications

Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

Mass spectrometry and tandem mass spectrometry characterization of protein patterns, protein markers and whole proteomes for pathogenic bacteria

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

Contact Info

Product

Resources

About