Species differences in the reaction of cattle to Jembrana disease virus infection

Soeharsono, S.; Wilcox, G. E.; Dharma, D.M.N.; Hartaningsih, Nining; Kertayadnya, G.; Budiantono, A.

doi:10.1016/s0021-9975(05)80020-9

Cited by 23 publications

(18 citation statements)

References 11 publications

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“…It is known that Bos taurus, Bos indicus and crossbred Bali (Bos javanicus x Bos indicus) cattle can be infected with JDV and remain viraemic for 3-6 months [3]. Viraemia has also been detected in the Indonesian buffalo (Bubalus bubalis) for at least 9 months after infection [6].…”

Section: Discussionmentioning

confidence: 99%

“…The principal lesions are an intense non-follicular proliferation of lymphoblastoid cells in lymphoid organs, and a lymphoid infiltrate in other visceral organs [2]. Infection of other bovine species induces only a mild or subclinical infection [3]. The disease is caused by Jembrana disease virus (JDV), a recently-described lentivirus that is antigenically and genetically related to, but distinguishable from, bovine immunodeficiency-like virus (BIV) [4,5].…”

Section: Introductionmentioning

confidence: 99%

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The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Soeharsono¹,

Wilcox

Putra³

et al. 1995

Epidemiol. Infect.

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SummaryMethods of transmission of Jembrana disease, an acute and severe disease of Bali cattle (Bos javanicus) caused by a recently-identified bovine lentivirus known as Jembrana disease virus, are described. During the acute disease virus can be detected in saliva and milk. There is evidence of direct transmission from acutely affected animals in close contact with susceptible cattle, possibly by virus in these secretions infecting cattle by the conjunctiva!, intranasal or oral routes, by which it was possible to infect cattle experimentally. During the acute disease the titre of infectious virus in blood is high, about 108 50% cattle infectious units (ID50)/ml, and it is probable that the virus is also transmitted mechanically by haematophagous arthropods. Recovered cattle are also a potential but probably infrequent source of infection; recovered cattle are persistently viraemic but the titre of infectious virus in blood decreases to about 101 ID50/ml by 60 days after recovery from the acute disease, and virus cannot be detected in secretions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Soeharsono¹,

Wilcox

Putra³

et al. 1995

Epidemiol. Infect.

Self Cite

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“…As negative samples, we used total RNA extracted from blood JDV non-infected Bali cattle and Ongole cattle. It is worthwhile mentioning that JDV infections of Ongole cattle only induce mild clinical signs and the infectious agents persist for less than 9 months [18,19]. Therefore the diagnosed animal may be healthy, or too few viruses are found in the sampled blood, or they have been rapidly eliminated.…”

Section: Discussionmentioning

confidence: 99%

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

et al. 2015

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Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on conserved tm subunit of Jembrana disease virus env gene. The RT-LAMP conditions were optimized by varying the concentration of MgSO 4 , betaine, dNTP, and temperature as well as the time and duration of reaction. The primers sensitivity for JDV was confirmed. The method was able to detect env-tm gene dilution which contained 2 9 10 -15 g of template. Comparatively, the sensitivity of RT-LAMP/LFD was 100-fold more sensitive than reverse transcription-polymerase chain reaction. The primers specificity for JDV was also confirmed using positive and negative controls. This work also showed that virus detection could be done not only on total RNA extracted from blood but various organs could also be analyzed for the presence of JDV using RT-LAMP/LFD method. The whole process, including the LAMP reaction and the LFD hybridization step only lasts approximately 75 min. Results of analysis can be easily observed with naked eyes without addition of any chemical or further analysis. The combination of RT-LAMP with LFD makes the method a more suitable diagnostic tool in conditions where sophisticated and expensive equipments are not available for field investigations on Jembrana disease in Bali cattle.

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“…Infection of Jembrana disease virus on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia (Kusumawati et al, 2014a). JDV has spread to almost west region in Indonesia (Soeharsono et al, 1995;Burkala et al, 1999) and has high prevalence which is more than 50% (Kusumawati et al, 2014b). Therefore it is very important to identify the presence of the virus as early as possible to prevent further disease transmission among the Bali cattle or outbreak to other areas.…”

Section: Discussionmentioning

confidence: 99%

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

Kusumawati¹,

Wanahari²,

Tampubolon³

et al. 2015

American Journal of Biochemistry and Biotechnology

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Jembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three different detection methods based on genomic approach, i.e., reverse transcriptase-polymerase chain reaction (RT-PCR), reverse transcriptase-loop mediated isothermal amplification (RT-LAMP) and dot-blot hybridization to detect JDV. Utilization of pGEX-TM, a recombinant plasmid containing env-tm gene as a positive control showed that RT-LAMP is the most sensitive method compares the two others. It could detect template concentration as low as 10 −6 ng µL −1 or equivalent to 1.52×10 2 plasmid copy number, 100 and 10000 more sensitive than RT-PCR and dot-blot hybridization, respectively.

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Species differences in the reaction of cattle to Jembrana disease virus infection

Cited by 23 publications

References 11 publications

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

The Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease Virus

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