Species diversity of the <scp><i>Culex pipiens</i></scp> complex in the Republic of Korea

Ryu, Jihun; Choi, Kwang Shik

doi:10.1111/1748-5967.12610

Cited by 6 publications

(7 citation statements)

References 40 publications

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“…Every year, Korea suffers from persistent outbreaks of mosquito-borne diseases mediated by the Anopheles and Culex genera, such as malaria and Japanese encephalitis [ 19 , 22 , 92 ]. Therefore, Korean mosquito research has prioritized the latter genera and has paid little attention to the Aedes genus [ 20 , 34 , 93 , 94 ]. However, Korea is facilitating the spread of potential vector mosquitoes owing to climate-change-induced increases in temperature and moist environmental composition [ 78 , 95 , 96 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Dengue Vector Aedes albopictus Collected from South Korea, Japan, and Laos

Shin

Rahman

Kim

et al. 2023

Insects

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Aedes albopictus is native to Southeast Asia and has emerged as a major vector for vector-borne diseases that are spreading rapidly worldwide. Recent studies have shown that Ae. albopictus populations have different genetic groups dependent on their thermal adaptations; however, studies on Korean populations are limited. In this study, we analyzed the genetic diversity and structure of two mitochondrial genes (COI and ND5) and sixteen microsatellites in mosquitoes inhabiting Korea, Japan, and Laos. The results indicate that the Korean population has low genetic diversity, with an independent cluster distinct from the Laos population. Mixed clusters have also been observed in the Korean population. On the basis of these findings, two hypotheses are proposed. First, certain Korean populations are native. Second, some subpopulations that descended from the metapopulation (East Asian countries) were introduced to Japan before migrating to Korea. Furthermore, we previously demonstrated that Ae. albopictus appears to have been imported to Korea. In conclusion, the dengue-virus-carrying mosquitoes could migrate to Korea from Southeast Asian epidemic regions, where they can survive during the severe winter months. The key findings can be used to establish an integrated pest management strategy based on population genetics for the Korean Ae. albopictus population.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Dengue Vector Aedes albopictus Collected from South Korea, Japan, and Laos

Shin

Rahman

Kim

et al. 2023

Insects

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“…Due to the difficulty in resolving the molecular identity of members of the Cx. pipiens complex with 16S rRNA , the acetylcholinesterase 2 region ( ace2 ) universal primers developed by Smith and Fonseca (2004) and species‐specific reverse primers by Ryu and Choi (2022) were utilized to distinguish between them. After species and gender identification, the mosquitoes were stored at −80°C until genomic DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

“…A 506‐bp sequence of the mitochondrial 16S rRNA was also amplified using the previously reported primer sets 16Sa (5′‐CGC CTG TTT ATC AAA AAC AT‐3′) and 16Sb (5′‐CTC CGG TTT GAA CTC AGA TCA‐3′) (Palumbi 2003) (Table 2). Primer information for Smith and Fonseca (2004) include F1457 (5′‐GAG GAG ATG TGG AAT CCC AA‐3′) and B1246S (5′‐TGG AGC CTC CTC TTC ACG G‐3′), while the species‐specific reverse primers by Ryu and Choi (2022) include ACEmole_R (5′‐TTC TCA CAG AGC CAT CAT CGA C‐3′), ACEpall_R (5′‐ACA TGT CAA AAG CTC AGT TAG T‐3′) and ACEquin_R (5′‐TGC CAC AGC CAT TCA AGA AGG‐3′). PCR amplifications were carried out in a 50‐μL volume reaction containing 3 μL genomic DNA, 5 μL 10× PCR buffer, 4 μL dNTPs mixture (2.5 mM), 1 μL of each primer (20 μM), 1 μL Taq DNA polymerase (5 U/μL) and 35 μL double‐distilled water.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Wolbachia endosymbiont in Culex molestus mosquitoes from South Korea

Akintola,

Kim,

Adeniyi

et al. 2024

Entomological Research

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Wolbachia, an endosymbiotic bacterium, has a significant impact on the reproductive biology and pathogen transmission capabilities of various arthropods, including mosquitoes. This study aimed to assess the prevalence of Wolbachia in Culex molestus, a vector mosquito species, in South Korea. Mosquito samples were collected from different locations and taxonomically identified. Individual mosquito DNA was extracted, and polymerase chain reaction (PCR) was conducted targeting the Wolbachia surface protein gene (wsp) and three multi‐locus sequence typing (MLST) (gatB, hcpA, and ftsZ) genes. Gel electrophoresis was performed to determine the prevalence of Wolbachia by analyzing PCR products. Our findings revealed that Wolbachia was present in 100% of the tested Culex mosquitoes from South Korea, with one major Wolbachia strain (supergroup B) detected using the wsp gene and subgrouping determined with the gatB as well as hcpA, and ftsZ. These results underscore the widespread occurrence of Wolbachia in Cx. molestus mosquito within the studied region, thus suggesting the potential for leveraging this endosymbiont to devise novel strategies for mosquito‐borne disease control. Future research should investigate the influence of Wolbachia on mosquito biology and vector competence in South Korea.

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“…pallens and Cx. pipiens f. molestus , following the method described by Ryu and Choi 12 . Universal forward primers for Cx.…”

Section: Methodsmentioning

confidence: 99%

“…pallens and Cx. pipiens f. molestus are currently identified in the ROK 12 . Despite their close evolutionary relationship, they exhibit distinct ecological differences 13 .…”

Section: Introductionmentioning

confidence: 99%

Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea

Jeon,

Lee,

et al. 2024

Sci Rep

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Two members of the Culex pipiens subgroup, Culex pallens and Culex pipiens f. molestus, are known to occur in the Republic of Korea (ROK). These species exhibit morphologically similar features and are challenging to distinguish below the species level. Therefore, this study utilized wing geometric morphometrics (GM) on the right wing of the Culex pipiens subgroup, alongside sequencing of the cytochrome c oxidase subunit I (COI) region. Mosquitoes were collected from 11 locations between June and October (2020–2022) to minimize regional and seasonal variations. Additionally, Culex pipiens f. pipiens, which is not native to the ROK, was included in the analysis. Culex tritaeniorhynchus, Aedes albopictus, and Anopheles sinensis, the primary vectors in the ROK, were used as outgroups for comparison. All three taxa in the Culex pipiens subgroup could be identified with an 82.4%–97.0% accuracy using GM. However, a comparison of the COI regions of the Culex pipiens subgroup revealed no clear differences between the taxa. These data can be used for accurate identification, contributing to effective mosquito control, in addition to providing a foundation for evolutionary and ecological studies on wing shape differences.

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Species diversity of the Culex pipiens complex in the Republic of Korea

Cited by 6 publications

References 40 publications

Genetic Diversity of Dengue Vector Aedes albopictus Collected from South Korea, Japan, and Laos

Genetic Diversity of Dengue Vector Aedes albopictus Collected from South Korea, Japan, and Laos

Prevalence of Wolbachia endosymbiont in Culex molestus mosquitoes from South Korea

Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea

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