Specific and selective electrochemical immunoassay for Pseudomonas aeruginosa based on pectin–gold nano composite

Krithiga, N.; Viswanath, Kamatchirajan Balaji; Vasantha, Vairathevar Sivasamy; Jayachitra, A.

doi:10.1016/j.bios.2015.12.006

Cited by 44 publications

(28 citation statements)

References 50 publications

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“…[ 42 ] Finally, monoclonal IgG antibodies were immobilized on the surface of a conventional Glassy carbon (GC) electrode modified with cross‐linked pectin (CCLP, conventional biomaterial) on Au nanoparticle. [ 43 ] Electrochemical PA sensing resulting from the binding of IgG to the immune complex of hydro quinone (HQ) and H 2 O 2 in PBS, lead to a LOD of 9 × 10 2 CFU per mL. [ 43 ]…”

Section: Electrochemical Detection Of Pamentioning

confidence: 99%

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Emerging Technologies for the Electrochemical Detection of Bacteria

McEachern

Harvey

Merle

2020

Biotechnology Journal

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Infections are a huge economic liability to the health care system, although real‐time detection can allow early treatment protocols to avoid some of this cost and patient morbidity and mortality. Pseudomonas aeruginosa (PA) is a drug‐resistant gram‐negative bacterium found ubiquitously in clinical settings, accounting for up to 27% of hospital acquired infections. PA secretes a vast array of molecules, ranging from secondary metabolites to quorum sensing molecules, of which many can be exploited to monitor bacterial presence. In addition to electrochemical immunoassays to sense bacteria via antigen–antibody interactions, PA pertains a distinct redox‐active virulence factor called pyocyanin (PYO), allowing a direct electrochemical detection of the bacteria. There has been a surge of publications relating to the electrochemical tracing of PA via a myriad of novel biosensing techniques, materials, and methodologies. In addition to indirect methods, research approaches where PYO has been sensitively detected using surface modified electrodes are reviewed and compared with conventional PA‐sensing methodologies. This review aims at presenting indirect and direct electrochemical methods currently developed using various surface modified electrodes, materials, and electrochemical configurations on their electrocatalytic effects on sensing of PA and in particular PYO.

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Section: Electrochemical Detection Of Pamentioning

confidence: 99%

“…[ 43 ] Electrochemical PA sensing resulting from the binding of IgG to the immune complex of hydro quinone (HQ) and H 2 O 2 in PBS, lead to a LOD of 9 × 10 2 CFU per mL. [ 43 ]…”

Section: Electrochemical Detection Of Pamentioning

confidence: 99%

Emerging Technologies for the Electrochemical Detection of Bacteria

McEachern

Harvey

Merle

2020

Biotechnology Journal

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“…Most of the detection schemes reported for P. aeruginosa focus on whole pathogen detection [ 142 – 146 ] with the work of Melanie et al [ 139 ], discussed above, on 16s rRNA detection being an outlier. In addition, to oligonucleotide recognition elements [ 139 , 142 – 144 ], antibodies [ 145 , 147 ] and bacteriophages [ 146 ] have also been used for specific detection of P. aeruginosa .…”

Section: Analytesmentioning

confidence: 99%

Nanomaterial enabled sensors for environmental contaminants

2018

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The need and desire to understand the environment, especially the quality of one’s local water and air, has continued to expand with the emergence of the digital age. The bottleneck in understanding the environment has switched from being able to store all of the data collected to collecting enough data on a broad range of contaminants of environmental concern. Nanomaterial enabled sensors represent a suite of technologies developed over the last 15 years for the highly specific and sensitive detection of environmental contaminants. With the promise of facile, low cost, field-deployable technology, the ability to quantitatively understand nature in a systematic way will soon be a reality. In this review, we first introduce nanosensor design before exploring the application of nanosensors for the detection of three classes of environmental contaminants: pesticides, heavy metals, and pathogens.

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“…Prospective medical applications of NMNPs/pectin materials as controlled release of nanoparticles, 40 targeted drug delivery, 34 singlet oxygen generation for photodynamic therapy, 38 theranostics, 18 and antibacterial/healing treatment 29 , 41 were reported. Other areas include surface-enhanced Raman spectroscopy (SERS) 42 and catalysis.…”

Section: Introductionmentioning

confidence: 99%

“… 19 , 27 The studies of the electrocatalytic properties of films formed by these materials were limited to AuNPs/pectin and explored for sensing. 25 , 31 , 41 , 43 , 44 …”

Section: Introductionmentioning

confidence: 99%

Noble Metal Nanoparticles in Pectin Matrix. Preparation, Film Formation, Property Analysis, and Application in Electrocatalysis

et al. 2020

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Stable polymeric materials with embedded nano-objects, retaining their specific properties, are indispensable for the development of nanotechnology. Here, a method to obtain Pt, Pd, Au, and Ag nanoparticles (ca. 10 nm, independent of the metal) by the reduction of their ions in pectin, in the absence of additional reducing agents, is described. Specific interactions between the pectin functional groups and nanoparticles were detected, and they depend on the metal. Bundles and protruding nanoparticles are present on the surface of nanoparticles/pectin films. These films, deposited on the electrode surface, exhibit electrochemical response, characteristic for a given metal. Their electrocatalytic activity toward the oxidation of a few exemplary organic molecules was demonstrated. In particular, a synergetic effect of simultaneously prepared Au and Pt nanoparticles in pectin films on glucose electro-oxidation was found.

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Specific and selective electrochemical immunoassay for Pseudomonas aeruginosa based on pectin–gold nano composite

Cited by 44 publications

References 50 publications

Emerging Technologies for the Electrochemical Detection of Bacteria

Emerging Technologies for the Electrochemical Detection of Bacteria

Nanomaterial enabled sensors for environmental contaminants

Noble Metal Nanoparticles in Pectin Matrix. Preparation, Film Formation, Property Analysis, and Application in Electrocatalysis

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