Specific Identification of Human Papillomavirus Type in Cervical Smears and Paraffin Sections by In Situ Hybridization with Radioactive Probes

Gupta, Jean W.; Gendelman, Howard E.; Naghashfar, Zohreh; Gupta, Prabodh K.; Rosenshein, Neil B.; Sawada, E; Woodruff, J. Donald; Shah, Keerti V.

doi:10.1097/00004347-198509000-00005

Cited by 73 publications

(16 citation statements)

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“…A possible role for human papillomavirus (HPV) in the induction of cervical cancer was first suggested by zur Hausen in 1976. Since then, ample data have been provided to demonstrate that certain HPV types (HPV6,11,16,18,31,33,35 and other types) exist in CIN lesions and in invasive cervical cancers (Grussendorf-Conen et al 1985, Gupta et al 1985, Crawford 1986, zur Hausen 1987, Syrjfinen et al 1986b, 1988. There is also morphological evidence of HPV in many cases of cervical intraepithelial neoplasis (CIN) (Meisels et al 1976, Syrjfinen 1984, 1986a, 1987c, 1987d.…”

Section: Introductionmentioning

confidence: 99%

“…In situ hybridization can detect HPV DNA in formalin-fixed and paraffinembedded specimens and this makes retrospective study of biopsy material possible (Gupta et al 1985, Syrjfinen 1986b, Syrj~nen 1988. In our study, we looked for the DNA of HPV types 6, 11, 16, 18, 31 and 33 in cervical precancer and cancer specimens obtained in Beijing, China in the late 1950's and early 1960's.…”

Section: Introductionmentioning

confidence: 99%

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In situ hybridization analysis of HPV DNA in cervical precancer and cervical cancers from China

Syrjänen²,

Syrjänen³

et al. 1990

Arch Gynecol Obstet

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A series of 103 cervical biopsies derived from 103 women during July 1958 to September 1963 from Beijing, China were investigated with in situ hybridization for the presence of HPV6, 11, 16, 18, 31 and 33 DNA. The mean age of the patients was 46.1 + 10.6 years with a range of 24-74 years. Morphological features of HPV infection were found in 80 (77.7%) biopsies. Invasive cervical cancer was diagnosed in 43 biopsies and cervical intraepithelial neoplasia CIN I, CIN II and CIN III in 9, 9, and 27 cases, respectively. A total of 63.1% (65/103) of the lesions had morphological features of HPV infections associated with CIN or invasive carcinomas. Altogether, 31.1% (32/103) of the biopsies were shown to contain HPV DNA. Of the cases showing HPV morphology, 43.1% were HPV DNA positive. HPV16 (30/32) was the most frequent type, followed by HPV11 and 18, whereas no lesions with HPV6, 31 or 33 were found. A total of 19/43 (44.2%) of the invasive carcinomas contained HPV DNA. HPV DNA positivity and the grade of CIN showed a statistically significant correlation (P = 0.0011). Our study demonstrated the presence of HPV in cervical lesions among Chinese women in the late 1950's and early 1960's when a single sexual partner was the rule and also supports the concept that HPV has as an important etiological role in cervical cancer, the highest risk being associated with HPV type 16. The applicability of in situ hybridization in retrospective assessment is emphasized.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In situ hybridization analysis of HPV DNA in cervical precancer and cervical cancers from China

Syrjänen²,

Syrjänen³

et al. 1990

Arch Gynecol Obstet

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“…Application of molecular techniques to cell samples is an adjunct to morphology that can be of assistance to the cytologist. Immunohistochemistry, 13,17 flow cytometry, 10,16,19,22 in situ hybridization, 11 fluorescence in situ hybridization 21 and PCR-based methods 4,7 have been applied to cytologic samples. In a recent study the utility of detecting expression of carcinoembryonic antigen by RT-PCR in peritoneal …”

Section: Discussionmentioning

confidence: 99%

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

Chuaqui

Cole²,

Cuello³

et al. 1999

Acta Cytologica

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“…Autoradiography has been used to detect radiolabelled probes in cell smears and paraffin sections (Gupta et al, 1985). Although autoradiographic techniques are very sensitive, the use of radiolabelled material is unsuitable for most routine applications.…”

Section: Discussionmentioning

confidence: 99%

In situ hybridization technique using an immunogold silver staining system

1989

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An in situ hybridization technique using an immunogold silver staining detection system was used to detect biotinylated DNA probes in cultured cells and in formalin-fixed paraffin-embedded tissue. The detection method is rapid, reliable and economical, producing an insoluble signal at the site of hybridization which is visible at low-power light microscopy and which can be enhanced by epipolarization microscopy.

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Specific Identification of Human Papillomavirus Type in Cervical Smears and Paraffin Sections by In Situ Hybridization with Radioactive Probes

Cited by 73 publications

References 0 publications

In situ hybridization analysis of HPV DNA in cervical precancer and cervical cancers from China

In situ hybridization analysis of HPV DNA in cervical precancer and cervical cancers from China

Analysis of mRNA Quality in Freshly Prepared and Archival Papanicolaou Samples

In situ hybridization technique using an immunogold silver staining system

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