Specific localization of epidermal-type fatty acid binding protein in dendritic cells of splenic white pulp

Abstract

Dendritic cells in the splenic white pulp of mice were intensely immunoreactive for epidermal-type fatty acid binding protein (E-FABP). This specific immunostaining revealed a clear difference in morphology between the dendritic cells in the periarterial lymphoid sheath (PALS) and follicular dendritic cells in the follicles in terms of cell sizes and process branching. No immunoreactivity was detected in dendritic cells in the marginal zones and the red pulp, although endothelial cells of almost all capillaries in the red pulp were immunoreactive for E-FABP. After peritoneal injection of lipopolysaccharide, the immunoreactive cells in PALS progressively enlarged and became rounded in shape with a peak in size at 24 h postinjection and they eventually resumed the dendritic form at 48 h postinjection. Within each of the enlarged immunoreactive cell perikarya were included small immunonegative apoptotic cells, presumptive lymphocytes. Taken together, E-FABP is useful as a marker for dendritic cells in the splenic white pulp, and may be involved through combination with fatty acids in antigen presentation and retention as well as in cytokine production.

Deficiency of Fatty Acid-Binding Proteins in Mice Confers Protection from Development of Experimental Autoimmune Encephalomyelitis

“…2A implicated DC as contributing to the protective effect of FABP deficiency in the EAE model. DC expression of both E-FABP and A-FABP has been reported previously (31,32). Given that both Aand E-FABP are expressed in macrophages, we considered it likely that myeloid-lineage DC express comparable levels of both A-and E-FABPs and that these FABPs function similarly in this closely related cell type.…”

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

“…However, its mRNA was later found to be expressed in various tissues including adipose tissue 5,8,9,[12][13][14][15][16][17][18][19][20][21][22][23] . Krieg et al 5) examined levels of C-FABP mRNA in mouse tissues, and found that the mRNA was most abundant in stratified epithelia of the epidermis and tongue.…”

“…The animals were housed individually in a room with controlled temperature (20)(21)(22), humidity (55-65%), and lighting (lights on from 07:00 to 19:00 h), and fed a laboratory chow (Type NMF, Oriental Yeast Co., Tokyo, Japan) for 1 week at least to acclimatize to our housing conditions. In a nutritional study to examine the effects of dietary fat types on gene expression, Sprague-Dawley rats obtained at 4 weeks of age were divided into groups after 1 week of acclimatization and were fed purified experimental diets containing 20% of different types of dietary fats for 21 d. The composition of the experimental diet was (in weight %): casein, 20; corn starch, 15; fat, 20; cellulose, 2; mineral mixture, 24) 3.5; vitamin mixture, 24) 1.0; DL-methionine, 0.3; choline bitartrate, 0.2 and sucrose, 38.…”

Differential Localization of Brain-Type and Epidermal-Type Fatty Acid Binding Proteins in the Adrenal Gland of Mice

“…Based on the idea that the exact localization of a given molecule is a first step and great help in understanding its functional significance, we have so far examined the localization of various types of FABPs in a series of studies (Iseki et al 1988;Watanabe et al 1991;Owada et al 1996aOwada et al , b, 1997Owada et al , 2001Owada et al , 2002aAbdelwahab et al 2003;Kitanaka et al 2003). Here we show that B-FABP is localized in the sustentacular cells enclosing the chromaffin cells in the adrenal medulla, while E-FABP is localized in a small number of presumptive dendritic cells distributed throughout the adrenal grand of postnatal mice.…”