Specific quorum sensing molecules of ammonia oxidizers and their role during ammonium metabolism in Zhalong wetland, China

Liu, Fengqin; Zhang, Yupeng; Liang, Hong; Gao, Dawen

doi:10.1016/j.scitotenv.2019.02.261

Cited by 28 publications

(7 citation statements)

References 30 publications

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“…The two AHLs used in this study were selected on the basis of their reported ability to increase the growth rate and microbial activity of different bacterial lineages ( 55 , 56 ). Other desired actions of AHLs can include enhancing metabolic activity, such as N-tetradecanoyl-DL-homoserine lactone or N-dodecanoyl-L-homoserine lactone in ammonium oxidizing bacteria ( 57 ), or inducing protection against oxidative stress, such as N-Decanoyl-DL-homoserine lactone in Burkholderia pseudomallei ( 58 ). These increased capabilities could give several bacteria a higher chance to withstand the change of environment to laboratory conditions and support their growth there.…”

Section: Discussionmentioning

confidence: 99%

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Lopez Marin,

Strejcek,

Uhlik

2023

Microbiol Spectr

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Bacteria need to be isolated in pure culture to gain access to the wide array of interesting functions they conceal. This is challenging because they do not live in isolation but instead in communities where they actively communicate and interact with each other. In this study, we aimed to increase the culturability of soil bacteria using three different signaling molecules: N-(3-oxohexanoyl)-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and 3',5'-cyclic adenosine monophosphate (cAMP). The signals were added individually to soils suspended in PBS buffer to a final concentration of 5 µM. Soil suspensions were agitated for 24 hours, after which they were serially diluted and plated on tenfold-diluted Reasoner’s 2A agar that either contained or did not contain the same signaling molecule used during the extraction step. DNA was isolated from both soil suspensions and grown cultures and, after a high-throughput amplicon sequencing, differences in bacterial abundances and diversity were determined across treatments. To further explain the action of the signaling molecules on the treated soil communities, their metagenomic functions were predicted using the software PICRUSt2. N-octanoyl-L-homoserine lactone was found to increase the diversity observed on solid media, while N-(3-oxohexanoyl)-L-homoserine lactone and cAMP were not. Potentially novel isolates aided by the signaling molecules were affiliated with the genera Pseudomonas and Nocardioides . IMPORTANCE Microorganisms are a repository of interesting metabolites and functions. Therefore, accessing them is an important exercise for advancing not only basic questions about their physiology but also to advance technological applications. In this sense, increasing the culturability of environmental microorganisms remains an important endeavor for modern microbiology. Because microorganisms do not live in isolation in their environments, molecules can be added to the cultivation strategies to “inform them” that they are present in growth-permissive environmental conditions. Signaling molecules such as acyl-homoserine lactones and 3',5'-cyclic adenosine monophosphate belong to the plethora of molecules used by bacteria to communicate with each other in a phenomenon called quorum sensing. Therefore, including quorum sensing molecules can be an incentive for microorganisms, specifically soil bacteria, to increase their numbers on solid media.

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Section: Discussionmentioning

confidence: 99%

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Lopez Marin,

Strejcek,

Uhlik

2023

Microbiol Spectr

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“…gingivalis, enhance the tolerance to drought in red rice plants in G. diazotrophicus PAL5, and enhance metabolic activity in AOB. − However, the functions of C 14 -HSL in other bacteria remain to be further studied. The C 14 -HSL signaling molecule was also detected in rhizobia; this AHL regulated biofilm formation in the Sinorhizobium fredii strain SMH12 but coregulated EPS production, surface translocation, motility, growth rate, and nodulation with other AHLs produced by S.…”

Section: Discussionmentioning

confidence: 99%

Detection, Structural Elucidation, and Biological Effects of Diverse N-Acyl-homoserine Lactone Signaling Molecules in the Plant-Promoting Endophytic Bacterium Rhizobium oryzihabitans M15

Zhao

Yang

et al. 2021

J. Agric. Food Chem.

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Quorum sensing (QS), usually performed by N-acyl-homoserine lactones (AHLs) in Gram-staining-negative bacteria, plays an important role in plant–bacteria interactions. Rhizobium oryzihabitans M15 is a plant-growth-promoting rhizobacterium (PGPR) isolated from rice roots. In this study, we found a QS system in the endogenous plasmid of R. oryzihabitans M15 and detected the activity of AHLs by a bioassay method. We identified five AHL analogues in R. oryzihabitans M15 using liquid chromatography-tandem mass spectrometry (LC-MS). The most dominant AHL analogue was N-(3R-hydroxy-7-cis-tetradecanoyl)-l-homoserine lactone according to nuclear magnetic resonance (NMR) and Mosher’s reactions. Furthermore, the rosI mutant abolished AHL production and significantly decreased growth, exopolysaccharide (EPS) production, biofilm formation, and motility compared to the wild-type strain. These results lay the foundation for further investigating the QS regulation mechanism and signal pathway of R. oryzihabitans M15 and its interactions with the host plant.

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“…Unlike ethanol-feed stimulation and the addition of conductive materials and electron shuttles, , our study provides the first proof of concept to drive DIET-based methanogenesis by the novel pathway of enhancing QS and expands DIET theory greatly. Moreover, QS widely occurs in various natural methanogenesis hotspots of paddy soils, wetland soils, and ocean sediments . In these various sediments, electroactive bacteria, e.g., Geobacter , and methanogens such as Methanosaeta , are widely detected and play important roles in carbon cycling. ,, QS is expected to regulate the DIET-based methanogenesis between electron-donating syntrophs and methanogens and affect the carbon cycling from a geoscience point of view.…”

Section: Environmental Implicationsmentioning

confidence: 99%

Quorum Sensing Enhances Direct Interspecies Electron Transfer in Anaerobic Methane Production

Zhao,

Zhu,

Liu

et al. 2024

Environ. Sci. Technol.

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Direct interspecies electron transfer (DIET) provides an innovative way to achieve efficient methanogenesis, and this study proposes a new approach to upregulate the DIET pathway by enhancing quorum sensing (QS). Based on longterm reactor performance, QS enhancement achieved more vigorous methanogenesis with 98.7% COD removal efficiency. In the control system, methanogenesis failure occurred at the accumulated acetate of 7420 mg of COD/L and lowered pH of 6.04, and a much lower COD removal of 41.9% was observed. The more significant DIET in QS-enhancing system was supported by higher expression of conductive pili and the c-Cyts cytochrome secretion-related genes, resulting in 12.7-and 10.3-fold improvements. Moreover, QS enhancement also improved the energy production capability, with the increase of F-type and V/A-type ATPase expression by 6.3-and 4.2-fold, and this effect probably provided more energy for nanowires and c-Cyts cytochrome secretion. From the perspective of community structure, QS enhancement increased the abundance of Methanosaeta and Geobacter from 54.3 and 17.6% in the control to 63.0 and 33.8%, respectively. Furthermore, the expression of genes involved in carbon dioxide reduction and alcohol dehydrogenation increased by 0.6-and 7.1fold, respectively. Taken together, this study indicates the positive effects of QS chemicals to stimulate DIET and advances the understanding of the DIET methanogenesis involved in environments such as anaerobic digesters and sediments.

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Specific quorum sensing molecules of ammonia oxidizers and their role during ammonium metabolism in Zhalong wetland, China

Cited by 28 publications

References 30 publications

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Detection, Structural Elucidation, and Biological Effects of Diverse N-Acyl-homoserine Lactone Signaling Molecules in the Plant-Promoting Endophytic Bacterium Rhizobium oryzihabitans M15

Quorum Sensing Enhances Direct Interspecies Electron Transfer in Anaerobic Methane Production

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