Specificity of attachment of fungal parasites to their hosts

Manocha, M. S.; Chen, Y.

doi:10.1139/m90-014

Cited by 44 publications

(18 citation statements)

References 42 publications

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“…The nature of the membrane is considered an important factor, since fungal hyphae are able to recognize topographical features of surfaces on which they grow, a recognition event which has been shown to be crucial to the development of infection structures (Wynn, 1976;Hoch et al, 1987;Allen et al, 1991). Moreover, it has been demonstrated that, at earlier stages, the mechanosensitivity of fungal hyphae,, alone or in addition to chemical recognition, could determine the specificity of surface adhesion (Manocha & Chen, 1990). In the present study, differences in membrane pore-size did not induce changes in fungal behaviour, suggesting that the different characteristics of membrane surface do not act as signals for AM fungi.…”

Section: Assessment Of Fungal Responses To Host-derived Signals Diffumentioning

confidence: 41%

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

et al. 1996

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SUMMARYAn experimental model for the study of recognition responses by arbuscular mycorrhizal symbionts to host roots was validated by analysing the main factors which might affect such responses. Results showed that fungal responses to bost-derived signals were unaffected by the degree of sterility of the system, host age and mycorrhizal status, symbiont age, or different chemical and physical characteristics of hydrophilic membranes. Use of dialysis membranes showed that the molecules putatively eliciting fungal responses weigh less than 500 Da. Utilization of the model for studies of developmental expression of proteins and genes in arbuscular mycorrhizal symbionts is examined.

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Section: Assessment Of Fungal Responses To Host-derived Signals Diffumentioning

confidence: 41%

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

et al. 1996

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“…Recent reports on attachment in fungal hyperparasites have often shown that metabolic activity is necessary for the successful recognition of the host by the pathogen (e.g. Manocha & Chen, 1990). The same has been demonstrated for the recognition and attachment of Rhizobium melilotii to the roots of legumes before nodulation (Lerouge et al, 1990).…”

Section: Tabte 2 Attachment and Germination* Of Discula Umbrinella Umentioning

confidence: 94%

“…Rees & Jones, 1984;Carver & Ingerson, 1987;Hamer et al, 1988). Manocha & Chen (1990) have noted, however, that the first contact of a fungal spore with the host surface is often a random event, with the parasite establishing numerous contacts with host and non-host surfaces but failing to adhere to the surface of non-hosts. In D. umbrinella, conidia may germinate on almost all surfaces used in this study, thus suggesting that germination is not necessarily subject to the evolutionary selection demonstrated for H. fragiforme by Chapela et al (1990;.…”

Section: Tabte 2 Attachment and Germination* Of Discula Umbrinella Umentioning

confidence: 99%

Differential attachment by conidia of the endophyte, Discula umbrinella (Berk. & Br.) Morelet, to host and non‐host surfaces

Toti

Viret

Chapela³

et al. 1992

New Phytologist

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SUMMARYDiflFerential attachment of conidia to leaves of different species (host and non-host) has been observed in the endophytic fungus, Discula umbrinella (Berk. & Br.) Morelet, a deuteromycete known to cause occasional epidemics of anthracnose in beech stands. On beech leaves, attachment gradually increased over 24 h and reached a maximum after 16-24 h; there were small differences in attachment between conidia suspended in water and those in HEPES buffer. The range of variation of results obtained with conidia suspended in HEPES was smaller but the adhesion was clearly enhanced. The adhesion of conidia to the host surface was highest at pH 5, and decreased with increasing pH. Heat and chemical treatments significantly reduced attachment. Two factors (or groups of factors) are likely to be involved in the recognition process. The first is heat-labile, the second one could be a non-protein molecule present on the conidial surface and actively recognized by the host. The physiological similarity between endophytes and other symbionts is briefly discussed.

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“…This crucial event leads to a differentiation process which results in the formation of infection structures such as appressoria, hyphal coils and hook-like bodies. Eventually, extracellular hydrolytic enzymes are secreted and penetration of the host takes place (Chet, 1987(Chet, ,1990Manocha & Chen, 1990;Tunlid e t al., 1992).…”

Section: Introductionmentioning

confidence: 99%

A newly isolated lectin from the Plant pathogenic fungus Sclerotium roltsii: purification, characterization and role in mycoparasitism

Inbar

Chet

1994

Microbiology

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A novel lectin was isolated and purified from the culture filtrate o f the PO Box 12, Rehovot 76100, Israel column with the flow-through, whereas all the non-agglutinating proteins present in the crude preparation remained bound t o the column until elution in a NaCl gradient. SDS-PAGE analysis o f the agglutinating fraction revealed a single band corresponding to a protein with a molecular mass of approximately 45 kDa. Agglutination o f Escherichia coli cells by the purified lectin was not inhibited by any o f the mono-or disaccharides tested, whereas the glycoproteins mr.icin and asialomucin did inhibit agglutination. Proteases, as well as 1,3-/?-glucanase, were found t o be totally destructive t o agglutination activity, indicating that both protein and 1,3-/?-glucan are necessary for agglutination. Using a biomimetic system based on binding o f the lectin to the surface o f inert nylon fibres revealed that the presence o f the purified agglutinin on the surface of the fibres specifically induced mycoparasitic behaviour in Trichoderma harzianum. Trichoderma formed tightly adhering coils, which were significantly more frequent with the purified agglutinin-treated fibres than with untreated ones or with those treated with non-agglutinating extracellular proteins from 5. rolfsii. Other mycoparasite-related structures, such as appressorium-like bodies and hyphal loops, were only observed in the interaction between T. harzianum and the purified agglutinin-treated fibres.

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Specificity of attachment of fungal parasites to their hosts

Cited by 44 publications

References 42 publications

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

Analysis of factors involved in fungal recognition responses to host‐derived signals by arbuscular mycorrhizal fungi

Differential attachment by conidia of the endophyte, Discula umbrinella (Berk. & Br.) Morelet, to host and non‐host surfaces

A newly isolated lectin from the Plant pathogenic fungus Sclerotium roltsii: purification, characterization and role in mycoparasitism

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