SPECT and PET to optimize cardiac stem cell therapy

Chan, Angel; Abraham, M. Roselle

doi:10.1007/s12350-011-9485-6

Cited by 18 publications

(20 citation statements)

References 77 publications

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“…PET imaging depicts the biological activity of molecular events and is particularly well suited for the assessment of early changes in disease pathophysiology. In this imaging modality, cells are either labeled directly with a radionuclide or by reporter gene labeling [167]. In the previously mentioned study from our lab in which S-TRAIL and HSV-TK were utilized to treat mouse models of GBM, the systemic administration of GCV post-tumor treatment and subsequent selective elimination of therapeutic MSC expressing HSV-TK in vitro and in vivo was monitored in real time by PET imaging utilizing 18F-FHBG, a substrate for HSV-TK [101].…”

Section: Other Imaging Techniquesmentioning

confidence: 99%

Genetically Engineered Mesenchymal Stem Cells

Shah

2014

Mesenchymal Stem Cells in Cancer Therapy

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Section: Other Imaging Techniquesmentioning

confidence: 99%

Genetically Engineered Mesenchymal Stem Cells

Shah

2014

Mesenchymal Stem Cells in Cancer Therapy

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“…61,62 PET is an imaging method that uses shortlived radioactive isotopes to qualitatively and quantitatively assess the biodistributions of targeting molecules in vivo. PET has a high spatial resolution, enhanced sensitivity, and dynamic imaging capabilities, but is very expensive because cyclotrons are needed to produce short-lived radionuclides for PET imaging.…”

Section: Current Stem Cell Imaging Technologiesmentioning

confidence: 99%

Molecular imaging for In vivo tracking of stem cell fate

et al. 2014

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Stem cells have a potential to dramatically change the common modalities of treatment for many diseases. Despite their rapid transition from animal studies to clinical applications, a number of unanswered questions remain regarding in vivo behaviors of stem cells transplanted into target tissues, including questions about their survival, distribution, migration, differentiation, and tumorigenicity. Recent advances in noninvasive molecular imaging technologies, including optical imaging, magnetic resonance imaging (MRI), radionuclide imaging, and reporter genebased imaging, have gradually elucidated the fundamental behaviors of stem cells via in vivo real time qualitative and quantitative monitoring. Here, we briefly review current imaging techniques for tracking stem cells, with an emphasis on the advantages and drawbacks of each imaging approach, and discuss future prospects for in vivo tracking of stem cells in regenerative medicine.

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“…Molecular imaging enables in vivo tracking of the distribution and long-term viability of stem cells by labeling cells with specific markers, including iron particles, radionuclides, and reporter genes. Labeled stem cells can be visualized noninvasively using multiple imaging modalities, such as MRI (17), single photon emission tomography (SPECT), positron emission tomography (PET) (16), and bioluminescent imaging (BLI) (22).…”

Section: Molecular Imaging For the Survival And Kinetics Of Engraftedmentioning

confidence: 99%

“…When compared with MRI, PET and SPECT have significantly higher sensitivity despite a lower spatial resolution. However, a major concern of using radioactive labeling is radiation exposure (16), which may inhibit cell viability and differentiation. A recent study in a rat model of MI demonstrated significant reduced proliferation and function of 99m Tc-hexamethylpropylenamine oxime-labeled human hematopoetic progenitor cells despite accurate homing to the infarcted myocardium (52).…”

Section: Molecular Imaging For the Survival And Kinetics Of Engraftedmentioning

confidence: 99%

“…Another problem lies in the short half-life of the radiotracer, which may limit the duration for long-term cell tracking (a couple of hours in the case of 18 FDG and a few days in the case of 111 In and 64 Cu). Additionally, a potential limitation is the label loss by diffusion or cell death with uptake of the label by neighboring viable cells (16).…”

Section: Molecular Imaging For the Survival And Kinetics Of Engraftedmentioning

confidence: 99%

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Mechanistic molecular imaging of cardiac cell therapy for ischemic heart disease

Fan

Cao

2013

American Journal of Physiology-Heart and Circulatory Physiology

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Yu Q, Fan W, Cao F. Mechanistic molecular imaging of cardiac cell therapy for ischemic heart disease. Am J Physiol Heart Circ Physiol 305: H947-H959, 2013. First published July 26, 2013 doi:10.1152/ajpheart.00092.2013.-Cell-based myocardial regeneration has emerged as a promising therapeutic option for ischemic heart disease, though not yet at the level of routine clinical utility. Despite the encouraging results from initial preclinical studies that have demonstrated improved function and reduced infarct size of the ischemic myocardium following several candidate cell transplantation, the beneficial effects and molecular mechanisms of cardiac cell therapy are still unclear in clinical applications to date, and much remains to be optimized. To improve engraftment, accurate methods are required for tracking cell fate and quantifying functional outcome. In the present review, we summarized the current status and challenges of cardiac cell therapy for ischemic heart disease and discussed the strengths and limitations of currently available in vivo imaging techniques with special focus on the newly developed multimodality approaches for assessing the efficacy of engrafted donor cells. We also addressed the hurdles these imaging modalities are facing, including issues regarding immunogenicity and tumorigenicity of transplanted stem cells, and provided some the future perspectives on stem cell imaging. molecular imaging; stem cell therapy; ischemic heart disease THIS ARTICLE is part of a collection on Physiological Basis of Cardiovascular Cell and Gene Therapies. Other articles appearing in this collection, as well as a full archive of all collections, can be found online at http://ajpheart.physiology. org/.

show abstract

SPECT and PET to optimize cardiac stem cell therapy

Cited by 18 publications

References 77 publications

Genetically Engineered Mesenchymal Stem Cells

Genetically Engineered Mesenchymal Stem Cells

Molecular imaging for In vivo tracking of stem cell fate

Mechanistic molecular imaging of cardiac cell therapy for ischemic heart disease

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