Spectrofluorimetric analysis of cefuroxime in pharmaceutical dosage forms

Murillo, J. A.; Lemus, J. M.; García, Luciano

doi:10.1016/0731-7085(94)e0018-v

Cited by 30 publications

(11 citation statements)

References 12 publications

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“…Although, several methods have been developed to determine cefuroxime axetil using other solvents such as (0.1 N NaOH, 0.1N HCl, combination of methanol and pH 7.0 phosphate buffer in ratio of 2:8) as shown in Table 4. [20][21][22], this is for the first time we used methanol, a cheap and most available solvent to develop the method. It does not require expensive or sophisticated instrument and chemicals in contrast with other chromatographic methods such as HPLC.…”

Section: Resultsmentioning

confidence: 99%

“…The estimation of cefuoxime axetil by mercurimetric method [11], high performance liquid chromatography [HPLC] [12][13][14][15][16], electrokinetic [17], high performance thin layer chromatography [HPTLC] [18,19], and spectroflurimetric [20] method is reported in literature. Although simultaneous UV spectrophotometric estimation of cefuroxime axetil has been reported by many analyst in bulk and in pharmaceutical formulation with 0.1N NaOH, 0.1N HCl and combination of methanol and pH 7.0 phosphate buffer in ratio of (2:8) as solvent [21][22][23].…”

Section: Cefuroxime Axetil Is Chemically [6r-[6α7β(z)]]-3-[[(2-aminomentioning

confidence: 99%

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Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

2013

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The present study was undertaken to develop and validate a simple, sensitive, accurate, precise and reproducible UV spectrophotometric method for cefuroxime axetil using methanol as solvent. In this method the simple UV spectrum of cefuroxime axetil in methanol was obtained which exhibits absorption maxima (λ max ) at 278 nm. The quantitative determination of the drug was carried out at 278 nm and Beer's law was obeyed in the range of (0.80-3.60) µg/ml. The proposed method was applied to pharmaceutical formulation and percent amount of drug estimated (95.6% and 96%) was found in good agreement with the label claim. The developed method was successfully validated with respect to linearity, specificity, accuracy and precision. The method was shown linear in the mentioned concentrations having line equation y = 0.05x + 0.048 with correlation coefficient of 0.995. The recovery values for cefuroxime axetil ranged from 99.85-100.05. The relative standard deviation of six replicates of assay was less than 2%. The percent relative standard deviations of inter-day precision ranged between 1.45-1.92% and intra-day precision of cefuroxime axetil was 0.96-1.51%. Hence, proposed method was precise, accurate and cost effective.

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Section: Resultsmentioning

confidence: 99%

Section: Cefuroxime Axetil Is Chemically [6r-[6α7β(z)]]-3-[[(2-aminomentioning

confidence: 99%

Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

2013

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“…Cefuroxime was investigated in blood samples by high pressure liquid chromatography (HPLC) [1], by micellar electrokinetic capillary chromatography and HPLC after pretreatment [2], in urine and bile by capillary zone electrophoresis [3] but also in dosage forms using HPLC [4] or spectrophotometric and spectrofluorimetric methods [5,6].…”

Section: Introductionmentioning

confidence: 99%

Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer

Zivanovic¹,

Ivanović²,

Vladimirov³

et al. 2004

Journal of Chromatography B

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“…A detailed survey of analytical literature for CEF revealed several methods based on varied techniques, viz, HPLC 11,12,13 , Spectrophotometery 14,15,16 , Spectrofluorimetry 17 and specific stability-indicating method by UV-Visible method 18 . Similarly, a survey of the analytical literature for sul revealed several methods based on varied techniques, viz HPLC 19,20,21,22 , Spectrophotometery 23,24,25 , HPTLC 26 .…”

Section: Introduction: Cefuroxime Sodium Is Sodium(7r)mentioning

confidence: 99%

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2012

IJPSR

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Cefuroxime is a 2 nd -generation cephalosporin and Sulbactam is a β-Lactamase inhibitor. The combination formulation is used for the treatment of lower respiratory tract infection. Two new, simple, accurate and precise UV spectrophotometric methods have been developed and validated for the simultaneous determination of Cefuroxime Sodium (CEF) and Sulbactam Sodium (SUL) in their combined dosage forms. First method is based on simultaneous estimation of Cefuroxime at 279nm and Sulbactam at 259 nm, while other Q-absorption Ratio method using two wavelengths, 259nm (λmax of SUL) and 272nm (Isoabsorptive point). 0.01 N NaOH was the solvent used in all methods. Cefuroxime Sodium showed linearity in the range of 8-32μg/mL and Sulbactam sodium showed linearity in the range of 4-16μg/mL in all the methods. All methods were validated statistically and recovery studies were carried out. All methods were found to be accurate, precise and reproducible. These methods were applied to the assay of the drugs in marketed formulation, which were found in the range of 98.0% to 100.0% of the labelled value for both Cefuroxime and Sulbactam. Hence, the methods herein described can be successfully applied in quality control of combined pharmaceutical dosage forms.

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Spectrofluorimetric analysis of cefuroxime in pharmaceutical dosage forms

Cited by 30 publications

References 12 publications

Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

Investigation of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer

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