Spectrophotometric determination of copper(I) and cobalt(II) with ferrozine

Kundra, S.K.; Katyal, Mohan; Singh, R. P.

doi:10.1021/ac60347a022

Cited by 57 publications

(25 citation statements)

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“…In the present work, however, such tendency was not detected. Since ferrozine can form a water soluble complex with Cu + (Kundra et al, 1974), it is possible that lower amounts of Cu were also available for absorption by the plants in the Fe-starvation treatment, where ferrozine was added to the nutrient solution, than in the control treatment.…”

Section: Analysis Of Pixementioning

confidence: 99%

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Silveira

Oliveira

Sperotto

et al. 2007

Braz. J. Plant Physiol.

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Iron is an essential nutrient for plants. In aerobic conditions, Fe is highly unavailable for plant uptake, and Fe deficiency can be severe in plants grown in calcareous soils. In waterlogged soils, however, Fe availability increases and can reach toxic concentrations. Rice is an important staple crop worldwide and faces iron deficiency or excess, depending on the growth conditions. To contribute to the study of mechanisms involved in response to Fe deficiency and resistance to Fe excess, experiments were carried out with rice cultivars BR-IRGA 409 (I409, susceptible to Fe toxicity) and EPAGRI 108 (E108, resistant to Fe toxicity) grown in culture solutions and submitted to Fe excess, control concentration or deficiency (500, 6.5 or zero mg L-1 Fe, respectively). Analysis of shoot dry weight confirmed the resistance of E108 plants to Fe excess. Mössbauer spectroscopy analysis indicated the presence of four different Fe3+compounds. The parameters obtained match those expected for ferrihydrite, lepidocrocite (and/or citrate) and Fe-nicotianamine. Mineral concentrations were determined using the PIXE (Particle Induced X-Ray Emission) technique. E108 plants had lower Fe concentrations than I409 plants when exposed to excess Fe. Except for lower Mn levels in roots and shoots, the excess of Fe did not result in lower nutrient concentrations in the susceptible cultivar compared to the resistant one. I409 plants seem to be affected directly by Fe toxicity rather than by secondary effects on mineral nutrition, whereas E108 plants seem to make use of the avoidance mechanism in the resistance to Fe overload. Both cultivars responded to Fe deficiency with allocation of P from roots to shoots. In addition to being more resistant to iron overload, E108 plants seem to be more efficient in inducing Fe deficiency responses.

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Section: Analysis Of Pixementioning

confidence: 99%

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Silveira

Oliveira

Sperotto

et al. 2007

Braz. J. Plant Physiol.

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“…It is known that high concentrations of other sample components (e.g. silicates or metals other than Fe) can interfere with the Fe analysis (Kundra et al 1974;Anastácio et al 2008;Im et al 2013) yet also Fe extraction can be affected as shown for intermediate reaction products of the denitrification process (Klueglein & Kappler 2013;Yan et al 2015). Apart from the commonly used separation of solid and liquid phase prior to Fe extraction (Weber et al 2006;Chakraborty et al 2011), the protocol suggested by Klueglein & Kappler (2013), using 40 mM sulfamic acid instead of 1 M HCl as extracting agent has been proven to be highly efficient and quantitatively accurate for the extraction of poorly crystalline Fe from bacterial batch cultures containing nitrite (Klueglein & Kappler 2013;Klueglein et al 2015;Li et al 2015;Xiu et al 2016) and was successfully applied to sediment and slurry samples (Laufer et al 2016a;Robertson et al 2016).…”

Section: Implications and Recommendation For Protocol Applicationmentioning

confidence: 99%

“…The sequential application of extraction protocols using increasing concentrations of acids allows the extraction of several Fe fractions from the very same sample, providing detailed information about the Fe mineral composition (Tessier et al 1979;Heron et al 1994;Poulton & Canfield 2005). The quantification of extracted Fe is commonly done using spectrophotometric assays (Braunschweig et al 2012;Verschoor & Molot 2013) based on agents such as ferrozine (Stookey 1970) or phenanthroline (Clark 1962 (Stookey 1970;Kundra et al 1974) or high concentrations of Fe III diss (Im et al 2013 It was therefore suggested by Klueglein & Kappler (2013) to replace HCl with sulfamic acid for Fe extraction in samples potentially containing nitrite. Fe extraction using sulfamic acid has proven to be successful in lab systems such as culture media as well as for environmental samples (Li et al 2015;Laufer et al 2016a;Robertson et al 2016;Xiu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

A Revised Iron Extraction Protocol for Environmental Samples Rich in Nitrite and Carbonate

Schaedler

Kappler

Schmidt

2017

Geomicrobiology Journal

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For submission to Geomicrobiology JournalWet-chemical iron extraction is widely applied to quantify the mineral-bound ferriferous fraction of sediments and soils. As previously shown, this method is strongly affected by the composition of the soil/sediment. Samples enriched in mostly microbially produced nitrite require the removal of the nitrite-containing aqueous phase or the replacement of HCl with sulfamic acid as extractant. In this study, we show that sedimentary carbonate buffers sulfamic acid, inhibiting the stabilization of Fe(II) and effective extraction of iron. We, therefore, provide a revised extraction protocol which allows to preserve low pH conditions, leading to efficient iron extraction in nitrite-and carbonate-enriched samples.

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“…Because the reagent is specific for ferrous ions under the reaction conditions used (Attari and Jaselskis 1972), measurements of total iron content depend on prior reduction of any Fe 3 + present using 10°/o (w/v) hydroxylamine hydrochloride. Since copper, cobalt and nickel ions produce some interference at pH values close to neutral (Kundra et al 1974), the reaction mixture was re-equilibrated through addition of an ammonium acetate-ammonium hydroxide buffer (pH 8.7). Iron standards were prepared through serial dilutions of 1 mg/ml FeCl 3 ·6H 2 0 in the same 1 N HCI that samples were digested in.…”

Section: Ferrozine Reagent Was Obtained Through Hach Chemical Companymentioning

confidence: 99%

Nitrogen and iron interactions in filamentous cyanobacteria

Hutchins¹

2000

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Also investigated was the relationship between siderophore-mediated iron uptake and diazotrophy in Anabaena. It was found that the strain of Anabaena used in these experiments (PCC strain 7120) is unable to make siderophores and fix nitrogen simultaneously, suggesting that previous work linking these two capabilites to competitive dominance may be in error or misinterpreted.The final aspect of this problem which was examined was the possibilty 2 of gratuitous repression of Anabaena siderophore production by manganese in the manner previously described by other investigators in E. coli. No evidence for this type of molecular regulation was found, although maganese did appear to bind to siderophore in the water.

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Spectrophotometric determination of copper(I) and cobalt(II) with ferrozine

Abstract: Based on the selective reactivity of the compounds containing the ferroin grouping

Cited by 57 publications

References 4 publications

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

Influence of iron on mineral status of two rice (Oryza sativa L.) cultivars

A Revised Iron Extraction Protocol for Environmental Samples Rich in Nitrite and Carbonate

Nitrogen and iron interactions in filamentous cyanobacteria

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