Spectrophotometric determination of tryptophan in intact proteins by the acid ninhydrin method

Molnár-Perl, I.; Pintér-Szakács, M.

doi:10.1016/0003-2697(89)90005-5

Cited by 34 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An Alliance (Agilent 1100) high performance liquid chromatograph (HPLC) was used, and the 15 eluate was detected at 338 nm. Tryptophan was determined separately as described by Molnar-Perl and Pinter-Szakacs (1989) 27 .…”

Section: Amino Acid Analysismentioning

confidence: 99%

Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion

et al. 2012

View full text Add to dashboard Cite

Currently, great attention is being paid to the utilization of biomass, such as feather keratins. It is imperative to extract and dissolve keratins from animal keratinous materials for exploitation of innovative biopolymers. However, most of the current processes are based on strong acid and alkali hydrolysis, chemical cleavage and other violent reactions, which are not eco-friendly and/or result in severe degradation and destruction of feather keratins. In this study, high density steam flash-explosion (HDSF) as an innovative pretreatment of biomass was firstly employed to treat feather waste. In HDSF treatment, steam with a powerful seepage force first penetrates into fibrous tissues and cells of feathers, and then quickly expands and breaks free of the structure upon an explosive decompression at supersonic speed (within 0.0875 s). HDSF effectively destabilized β-sheet crystals and intermolecular disulfide bonds without causing substantial damage to the keratin protein chain, dramatically increasing the extraction and dissolubility of feather keratins in polar solvents like water, salt solution and weak bases, as well as enzymatic accessibility. HDSF treatment could be a sustainable and practical pretreatment for extraction of feather keratin for exploitation of biomaterials and conversion of feathers to nutrient animal feed instead of the current chemical hydrolysis and hydrothermal treatment.

show abstract

Section: Amino Acid Analysismentioning

confidence: 99%

Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Ninhydrin reagent is used for the determination of an aliphatic primary amine or an amino acid group (Friedman 2004;Rahman & Azmi 2001a;Campins-Falco et al 1996;Arayne et al 2008;Rahman & Azmi 2001b;Nobrega Jde et al 1994;Molnar-Perl & Pinter-Szakacs 1989). The presence of an aromatic ring exhibits the response; the exhibition increases if the amino group is nearer to the ring.…”

Section: Reaction With Ninhydrin (Nin)mentioning

confidence: 99%

Kinetic and Thermodynamic Spectrophotometric Technique to Estimate Gabapentin in Pharmaceutical Formulations using Ninhydrin

et al. 2013

View full text Add to dashboard Cite

Background: Simple and sensitive spectrophotometric method is described based on the reaction of drug (gabapentin) with ninhydrin in pure form and in pharmaceutical preparations. Methods: Complex formed during this reaction is measured at 575 nm as a function of time. Kinetic study involve initial-rate, rate-constant and fixed-time (80 minutes) procedures to determine the concentration of the drug.

show abstract

“…This, so-called 'acid-ninhydrin' method was optimized on the basic research level [8] and applied primarily to the fast determination of the tryptophan content of food and feed stuffs [9], without hydrolysis of the protein.…”

Section: Identification and Quantification Of Tryptophan In Intact Prmentioning

confidence: 99%

Spectrophotometric determination of tryptophan in intact proteins by the acid ninhydrin method

Cited by 34 publications

References 27 publications

Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion

Sustainable and practical utilization of feather keratin by an innovative physicochemical pretreatment: high density steam flash-explosion

Kinetic and Thermodynamic Spectrophotometric Technique to Estimate Gabapentin in Pharmaceutical Formulations using Ninhydrin

Advancements in the rapid and sensitive analyses of tryptophan and its metabolites, by chromatography

Contact Info

Product

Resources

About