Spectrum-Effect Relationships Between Chemical Fingerprints and Antibacterial Effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and Microcalorimetry

Abstract

The traditional Chinese medicines Lonicerae Japonicae Flos (LJF, Jinyinhua in Chinese) and Lonicerae Flos (LF, Shanyinhua in Chinese) refer to the flower buds of five plants belonging to the Caprifoliaceae family. Until 2000, all of these were officially listed as a single item, LJF (Jinyinhua), in the Chinese Pharmacopoeia. However, there have recently been many academic controversies concerning the separation and combination of LJF and LF in administrative regulation. Till now there has been little work completed evaluating the relationships between biological activity and chemical properties among these drugs. Microcalorimetry and UPLC were used along with principal component analysis (PCA), hierarchical cluster analysis (HCA), and canonical correlation analysis (CCA) to investigate the relationships between the chemical ingredients and the antibacterial effects of LJF and LF. Using multivariate statistical analysis, LJF and LF could be initially separated according to their chemical fingerprints, and the antibacterial effects of the two herbal drugs were divided into two clusters. This result supports the disaggregation of LJF and LF by the Pharmacopoeia Committee. However, the sample of Lonicera fulvotomentosa Hsu et S. C. Cheng turned out to be an intermediate species, with similar antibacterial efficacy as LJF. The results of CCA indicated that chlorogenic acid and 3,4-Dicaffeoylquinic acid were the major components generating antibacterial effects. Furthermore, 3,4-Dicaffeoylquinic acid could be used as a new marker ingredient for quality control of LJF and LF.

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

“…According to 'Ben Cao Gang Mu' (AD 1552-1578, the herbalism masterpiece which was known as the ancient Chinese encyclopedia, JYH was described as a commonly used herb to treat fever, phyma and sore. Modern pharmacological study has confirmed the antiviral, antibacterial and anti-inflammatory activities of JYH, supporting the traditional applications (Kang et al 2004;Kao et al 2015;Shi et al 2016). Likewise, pharmacological study showed antioxidative, anti-tumour, liver protective and hypoglycemic activities of JYH Kong et al 2017;Park et al 2012a).…”

Comparative study of chemical composition and active components against α‐glucosidase of various medicinal parts of Morus alba L.

“…Spectrum–effect relationship analysis is an emerging approach to facilitating the bioactivity‐relevant quality control of herbal medicines. There is no doubt that the spectrum–effect relationship approach fits very well with the holistic mode of herbal medicines, eliciting the pharmacological efficacy via several components (Liu, Wu, Huang, Liu, & Feng, ; Shi et al, ; Zhang, Chen, Yang, & Shi, ). In the present study, we established a spectrum–effect relationship model to identify a set of combinatorial marker compounds that might be representative of the bioactivity of Cortex Mori, Ramulus Mori, Folium Mori and Fructus Mori.…”

Spectrum–effect relationship study between HPLC fingerprints and antioxidant of honeysuckle extract

“…In addition, modern pharmacological research has discovered wide pharmacological actions, such as antiviral, anti‐inflammatory, antibacterial, antiendotoxin, antipyretic and antioxidative (Shang, Pan, Li, Miao, & Ding, ; Shi et al, ). Among these, the antioxidative activity makes a great contribution to its medicinal and edible value.…”

“…Considering that a multivariate statistical mathematical model can be employed to create links between chemicals and efficacy, the combination of spectrum and effect by statistical analysis can provide a new route for target components. Shi et al () screened the major antibacterial components of chlorogenic acid and 3,4‐dicaffeoylquinic acid from Lonicerae Japonicae Flos and Lonicerae Flos by spectrum–effect relationships. Therefore, in this paper, a spectrum–effect relationship study between HPLC fingerprints and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activity of honeysuckle methanol extract was carried out for potential antioxidant compounds.…”