Sperm dosage and site of insemination in relation to fertility in bovines

Mohanty, T. K.; Lone, Shabir Ahmad; Kumaresan, A.; Bhakat, Mukesh; Kumar, Raj; Baithalu, Rubina Kumari; Sinha, Rajesh Kumar; Paray, Adil Rasool; Yadav, Hanuman Prasad; Sahu, Shivani; Mohanty, Ashok Kumar

doi:10.4103/2305-0500.220977

Cited by 19 publications

(16 citation statements)

References 2 publications

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“…It is necessary to determine the sperm concentration as it dictates the dilution rate needed for further processing of semen, just like simple extension or cryopreservation. Sperm concentration is necessary to obtain acceptable fertility results crucial to the AI industry (Mohanty et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting in decreased sperm quality. ROS's destructive potential is normally counteracted in sperm by their innate antioxidant system consisting of enzymes, which include superoxide dismutase (SOD). This study aimed to assess the quality of semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation of total sperm motility (%) and determination of SOD activity (U/mL). Here, semen samples were collected from 12 sexually mature IP-Khaki drakes, an improved egg-type breed of Philippine mallard duck, and processed using modified reported cryopreservation procedure for ducks. Results showed that post-thawing total sperm motility averages of 12.04±5.61% using 4.5% DMSO and 13.99±5.28% using 7.0% glycerol were comparable. Moreover, similar SOD activity levels of 0.39±0.18 U/mL with 4.5% DMSO and 0.33±0.21 U/mL with 7.0% glycerol in 2.00 x 108 IP-Khaki sperm cells were also observed. The observed very low intracellular SOD activity indicates severe damage to sperm cells due to cryopreservation, which resulted in a comparably low total sperm motility with either of the cryoprotectants. Thus, the cryopreservation protocol used is not the optimum for IP-Khaki semen based on the observed considerable decline in sperm motility and very low SOD activity after cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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“…Therefore, findings of this study may suggest that sperm motility itself is not a suitable parameter to predict semen fertility, and it seems clear that other sperm characteristics should be considered to evaluate semen fertility potential. It is important to highlight that sperm motility evaluation should consider the sperm concentration (Mohanty et al, ). In this study, subjective sperm motility ranged from 30% to 75%, considering straws with 30 x 10 6 sperm, even the lowest sperm motility represented at least 9 x 10 6 of viable sperm cells which is considered as a satisfactory inseminating dose (Den Daas, Jong, Lansbergen, & Wagtendonk‐De Leeuw, ; Gérard & Humblot, ).…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination

Okano

Penitente-Filho

León

et al. 2019

Reprod Domestic Animals

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Contents This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows.

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“…Este proceso favorece cambios estructurales en el espermatozoide, mediado por sustancias llamadas «capacitantes» que promueven cambios estructurales y bioquímicos que conducen la eliminación de componentes adheridos a la membrana del espermatozoide, cambio de la composición lipídica de la membrana espermática, aumento de la permeabilidad a los iones Ca 2+ , cambio en el pH interno y un incremento en la permeabilidad y metabolismo celular ( Tras la selección espermática se determina la concentración, que permite conocer y ajustar la cantidad de espermatozoides que serán colocados con los ovocitos maduros. El conteo se realiza con hemocitómetro o cámara de Thoma (Anzar et al, 2009), y el ajuste suele realizarse a dosis que varían entre 1 y 6x10 6 espermatozoides/ml de medio de fecundación, dependiendo del protocolo utilizado (Mohanty et al, 2018). Tras la adición de los espermatozoides al medio de fertilización con los óvulos previamente ubicados, se consolida el cocultivo con los óvulos y espermatozoides en medio de fertilización, por periodos entre 18 y 22 horas en ambiente de alta humedad y protegidos de la luz (Parrish et al, 1986).…”

Section: Fertilización In Vitro (Fiv)unclassified

Aspectos esenciales sobre las técnicas de fertilización in vitro en bovinos

Salgado-Cruz

Vasquez

2020

Rev. investig. vet. Perú

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La producción de embriones in vitro incluye un conjunto de técnicas utilizadas para la multiplicación en condiciones de laboratorio de líneas de animales genéticamente superiores. Para la obtención de embriones en el laboratorio se requieren diferentes protocolos y equipos que simulen las condiciones naturales de desarrollo de los embriones en la madre, buscando lograr resultados eficientes. La producción de embriones consta de tres etapas, maduración in vitro (MIV) de los ovocitos, fecundación in vitro (FIV) y cultivo in vitro (CIV) de los posibles cigotos, buscando obtener blastocistos de calidad para una mayor eficiencia reproductiva y mejoramiento genético de los rebaños.

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Sperm dosage and site of insemination in relation to fertility in bovines

Cited by 19 publications

References 2 publications

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination

Aspectos esenciales sobre las técnicas de fertilización in vitro en bovinos

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