Spermatic and testicular damages in rats exposed to ethanol: Influence of lipid peroxidation but not testosterone

Siervo, Gláucia Elóisa Munhoz de Lion; Vieira, Henrique Rodrigues; Ogo, Fernanda Mithie; Fernandez, Carla Dal Bianco; Gonçalves, Géssica Dutra; Mesquita, Suzana F.P.; Anselmo-Franci, J. A.; Cecchini, Rúbens; Guarnier, Flávia Alessandra; Fernandes, Glaura Scantamburlo Alves

doi:10.1016/j.tox.2015.01.016

Cited by 49 publications

(63 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our sperm count results for the testis and epididymis corroborate our previous study on adult rats (Siervo et al, 2015). We suggest that the same dose of ethanol, for a period of 50 or 63 days of exposure to ethanol, affects sperm parameters at both peripubertal and adult age.…”

Section: Discussionsupporting

confidence: 90%

“…In relation to adult animals, the data from our laboratory (Siervo et al, 2015) revealed changes in spermatogenic dynamics, and were associated with a testicular sperm count reduction. Thus, pubescent animals appear to be more resistant.…”

Section: Discussionmentioning

confidence: 93%

“…This result indicates that ethanol acts on the epididymis and influences the sperm maturation process negatively, and may reduce the fertility of the individual momentarily or persistently. Adult rats exposed to ethanol also showed reduced sperm motility (Siervo et al, 2015;Srikanth et al, 1998).…”

Section: Discussionmentioning

confidence: 97%

“…The animals were randomly assigned to two experimental groups: control (C; n 5 12) and ethanol (E; n 5 12). The ethanol (E) group received ethanol (Nuclear V R , Diadema, Brazil, dose of 2 g/kg, 25% v/v), according to a previous study (Siervo et al, 2015). The control group received only the vehicle (water).…”

Section: Methodsmentioning

confidence: 99%

“…In this context, most studies are performed on mature animals (F avaro and Cagnon, 2008;Koh and Kim, 2006;Mesquita et al, 2013;Oliva et al, 2006;Srikanth et al, 1998Srikanth et al, , 1999Zhu et al, 2000). A previous study done by our laboratory (Siervo et al, 2015) with adult rats showed that ethanol 2 g/kg, 25% (v/v) for 30 consecutive days impaired parameters of sperm count, spermatogenic kinetics, lipid peroxidation levels, and sperm motility and morphology, and also caused histopathological damage in the testis and epididymis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ethanol exposure during peripubertal period increases the mast cell number and impairs meiotic and spermatic parameters in adult male rats

Punhagui

Vieira

Siervo

et al. 2016

Microsc. Res. Tech.

View full text Add to dashboard Cite

Puberty is characterized by psychosomatic alterations, whereas chronic ethanol consumption is associated with morphophysiological changes in the male reproductive system. The purpose of this study was to show the toxic effects on testis and epididymal morphophysiology after ethanol administration during peripuberty. To this end, male Wistar rats were divided into two groups: ethanol (E) group: received a 2 g dose of ethanol/kg in 25% (v/v); and control (C) group: received the same volume of filtered water; both were treated by gavage for 54 days. On the 55th day of the experiment, epididymis, and testis were collected for sperm count, histopathology, mast cell count, and morphometry. The vas deferens was collected for sperm motility analysis. The femur and testicle were used for cytogenetic analysis. Ethanol exposure caused reduction in daily sperm production (DSP) and in sperm motility, multinucleated cells or those having no chromosomal content, and late chromosome migrations. No changes were observed in the number of chromosomes in the mitotic analysis. However, some alterations could be seen in meiocytes at different stages of cell division. Stereological analysis of the epididymis indicated reorganization of its component in the 2A and 5A/B regions. The epididymal cauda had greater recruitment, and both degranulated and full mast cells showed an increase in the initial segment, in the ethanol group. In conclusion, ethanol administration during the pubertal phase affects epididymis and testis in adult rats, as indicated mainly by our new findings related to mast cell number and meiotic impact. Microsc. Res. Tech. 79:541-549, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ethanol exposure during peripubertal period increases the mast cell number and impairs meiotic and spermatic parameters in adult male rats

Punhagui

Vieira

Siervo

et al. 2016

Microsc. Res. Tech.

View full text Add to dashboard Cite

show abstract

Reproductive toxicity of maternal exposure to di(2‐ethylhexyl)phthalate and butyl paraben (alone or in association) on both male and female Wistar offspring

Guerra

Erthal

Punhagui-Umbelino

et al. 2022

J of Applied Toxicology

View full text Add to dashboard Cite

Parabens and phthalates are commonly found as contaminants in human fluids and are able to provoke reproductive toxicity, being considered endocrine disruptors. To evaluate the effects of phthalate and paraben, alone or in combination, on reproductive development of the offspring, female pregnant Wistar rats were allocated in six experimental groups: Three control groups (gavage [CG], subcutaneous [CS], and gavage + subcutaneous) received corn oil as vehicle, and the remaining groups were exposed to di(2-ethylhexyl)phthalate (DEHP) (500 mg/kg, gavage), butyl paraben (BP) (100 mg/kg, subcutaneously), or MIX (DEHP + BP), from Gestational Day 12 until Postnatal Day (PND) 21. The following parameters were assessed on the off-

show abstract

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Moradi,

Hashemian,

Fathi

et al. 2023

J Biochem & Molecular Tox

View full text Add to dashboard Cite

There is a growing concern that antidepressant drugs impair sexual function and adversely impact spermatogenesis and male fertility. Vitamin C is a natural antioxidant that plays a vital role in the male reproductive system. The present study investigated the ameliorating potential of vitamin C against citalopram (CTL)‐evoked testicular toxicity and spermatogenesis impairment in mice. Mice were randomly divided into six groups: control, CTL, vitamin C 100, vitamin C 200, CTL plus vitamin C 100, and CTL plus vitamin C 200. Adult male mice were intraperitoneally (ip) injected with 10 mg/kg of CTL for 35 days with or without vitamin C. At the end of the study, body and testes weight, sperm parameters, histopathology of testes, testosterone level, testicular levels of malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC), and apoptosis (TUNEL assay) were evaluated. Our findings revealed that vitamin C restored spermatogenesis by improving sperm count, motility, viability, morphology, and chromatin integrity. Testosterone levels and testes histopathology were significantly improved in the vitamin C‐administrated groups. Furthermore, vitamin C administration markedly alleviated CTL‐induced nitro‐oxidative damage, enhancing TAC levels, and reducing NO and MDA levels. Whilst CTL therapy induced a significant increase in the number of TUNEL‐positive cells compared to the control, the administration of vitamin C significantly prevented the apoptotic effects of CTL. Together, vitamin C therapy protects against CTL‐induced testicular damage via mitigating nitro‐oxidative stress and apoptosis, which provides evidence for vitamin C as a beneficial therapy against antidepressant drug‐associated reproductive toxicity and male sub/infertility.

show abstract

Spermatic and testicular damages in rats exposed to ethanol: Influence of lipid peroxidation but not testosterone

Cited by 49 publications

References 41 publications

Ethanol exposure during peripubertal period increases the mast cell number and impairs meiotic and spermatic parameters in adult male rats

Ethanol exposure during peripubertal period increases the mast cell number and impairs meiotic and spermatic parameters in adult male rats

Reproductive toxicity of maternal exposure to di(2‐ethylhexyl)phthalate and butyl paraben (alone or in association) on both male and female Wistar offspring

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Contact Info

Product

Resources

About