Spermatogonial stem cell sensitivity to capsaicin: An in vitro study

Mizrak, Sefika C.; Gadella, Bart M.; Erdost, Hatice; Özer, Aytekin; Pelt, Ans M.M. van; Dissel‐Emiliani, F. M. F. Van

doi:10.1186/1477-7827-6-52

Cited by 24 publications

(16 citation statements)

References 36 publications

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“…Li et al [60] did not find expression of TRPV1 in rat testicular tissue, however, they found TRPV5 in plasma membranes of spermatogenic cells. Studies performed in male mice and rats indicated that TRPV1 expression was mostly found in premeitoic germ cells, whereas spermatocytes only weakly expressed this receptor, while no expression was observed in postmeiotic germ cells [18,19]. Strong positive immunoreaction was visible in our study in the cytoplasm of multinucleated giant cells, formed within layers of TRPV-1-positive spermatocytes, and TRPV1-immunorectivity in germ cells was higher in the testis of letrozole-treated rats than in control.…”

Section: Discussionsupporting

confidence: 43%

“…Immunohistochemical and Western blot analyses localized TRPV1 in the plasma membrane of cultured two rat spermatogonial stem cells lines (Gc-5spg and Gc-6spg); the receptor was also immunolocalized in premeiotic germ cells both in undifferentiated and differentiated spermatogonia, and early spermatocytes in adult rat testis [18]. It was suggested, that TRPV1 plays a crucial role in the protection of germ cells against heat stress, preventing spermatogonia from undergoing massive cell death [19].…”

Section: Introductionmentioning

confidence: 99%

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The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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Abstract:The function of testis is under hormonal control and any disturbance of hormonal homeostasis can lead to morphological and physiological changes. Therefore the aim of the study was to investigate the expression of androgen and estrogen receptors (AR, ERs), vanilloid receptor (TRPV1), cytochrome P450 aromatase (P450arom), as well as apoptosis of cells in testis of adult rats chronically treated with letrozole (LT), a non-steroidal aromatase inhibitor, for 6 months. The testicular tissues were fixed in Bouin's fixative and embedded in paraffin. Immunohistochemistry with monoclonal antibodies (abs) against AR, ERa, P450arom, and polyclonal abs against ERb, TRPV1, caspase-3 was applied. Long-lasting estradiol deficiency, as an effect of LT treatment, produced changes in the morphology of testis and altered the expression of the studied receptors in cells of the seminiferous tubules and rate of cell apoptosis. The immunostaining for AR was found in the nuclei of Sertoli cells and the cytoplasm of spermatogonia and spermatocytes in III-IV stages of the seminiferous epithelium cycle. The intensity of staining for P450arom was lower in the testis of LT-treated rats as compared to control animals. The immunofluorescence of ERa and ERb was observed exclusively in the nuclei of Leydig cells of LT-treated rats. There were no changes in localization of TRPV1, however, the intensity of reaction was stronger in germ cells of the seminiferous epithelium after LT treatment. The apoptosis in both groups of animals was observed within the population of spermatocytes and spermatids in II and III stages of the seminiferous epithelium cycle. In testis of LT-treated rats the immunoexpression of caspase-3 was additionally found in the germ cells in I and IV stages, and Sertoli, myoid and Leydig cells. In conclusion, our results underline the important role of letrozole treatment in the proper function of male reproductive system, and additionally demonstrate that hormonal imbalance can produce the morphological abnormalities in testis.

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Section: Discussionsupporting

confidence: 43%

Section: Introductionmentioning

confidence: 99%

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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“…These results can be interpreted in the light of available in vitro data suggesting the involvement of CPS and CPZ in signaling pathways beyond TRPV1. In fact, it has been reported that long-term exposure of cells to CPS and/or short term stimulation with CPS in the high concentration range of 100 to 300 μM may trigger enzymatic processes, either in the plasma membrane or in the mitochondria of cells, that subsequently lead to cell death [42–44]. In parallel, it is well established that TRPV1 signaling converges with the endocannabinoid system in sperm cells that, in turn, exerts a modulating effect on fertilizing ability and acrosomal loss of human sperm [45, 46].…”

Section: Discussionmentioning

confidence: 99%

Heat Sensing Receptor TRPV1 Is a Mediator of Thermotaxis in Human Spermatozoa

et al. 2016

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The molecular bases of sperm thermotaxis, the temperature-oriented cell motility, are currently under investigation. Thermal perception relies on a subclass of the transient receptor potential [TRP] channels, whose member TRPV1 is acknowledged as the heat sensing receptor. Here we investigated the involvement of TRPV1 in human sperm thermotaxis. We obtained semen samples from 16 normozoospermic subjects attending an infertility survey programme, testis biopsies from 6 patients with testicular germ cell cancer and testis fine needle aspirates from 6 patients with obstructive azoospermia undergoing assisted reproductive technologies. Expression of TRPV1 mRNA was assessed by RT-PCR. Protein expression of TRPV1 was determined by western blot, flow cytometry and immunofluorescence. Sperm motility was assessed by Sperm Class Analyser. Acrosome reaction, apoptosis and intracellular-Ca2+ content were assessed by flow cytometry. We found that TRPV1 mRNA and protein were highly expressed in the testis, in both Sertoli cells and germ-line cells. Moreover, compared to no-gradient controls at 31°C or 37°C (Ctrl 31°C and Ctrl 37°C respectively), sperm migration towards a temperature gradient of 31–37°C (T gradient) in non-capacitated conditions selected a higher number of cells (14,9 ± 4,2×106 cells T gradient vs 5,1± 0,3×106 cells Ctrl 31°C and 5,71±0,74×106 cells Ctrl 37°C; P = 0,039). Capacitation amplified the migrating capability towards the T gradient. Sperms migrated towards the T gradient showed enriched levels of both TRPV1 protein and mRNA. In addition, sperm cells were able to migrate toward a gradient of capsaicin, a specific agonist of TRPV1, whilst capsazepine, a specific agonist of TRPV1, blocked this effect. Finally, capsazepine severely blunted migration towards T gradient without abolishing. These results suggest that TRPV1 may represent a facilitating mediator of sperm thermotaxis.

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“…Accordingly, the endogenous content of 2-AG, but not of AEA, markedly decreased during the differentiation process and was paralleled by lower or higher mRNA levels of 2-AG synthesizing or degrading enzymes, respectively (Grimaldi et al 2009b). In addition, it was suggested that a steady AEA tone in testes might activate TRPV1, thus protecting germ cells against testicular hyperthermia (Mizrak et al 2008). In line with this, experiments performed with Trpv1 -/mice demonstrated that this receptor promotes apoptosis of spermatogonia and blocks the progression of germ cell meiosis, as a defensive mechanism against abnormally elevated testicular temperature (Mizrak et al 2008).…”

Section: Spermatogenesis and Spermiogenesismentioning

confidence: 93%

Endocannabinoids

2015

Handbook of Experimental Pharmacology

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Spermatogonial stem cell sensitivity to capsaicin: An in vitro study

Cited by 24 publications

References 36 publications

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Heat Sensing Receptor TRPV1 Is a Mediator of Thermotaxis in Human Spermatozoa

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