Spherical Phospholipid Polymer Hydrogels for Cell Encapsulation Prepared with a Flow-Focusing Microfluidic Channel Device

Aikawa, Tatsuo; Konno, Tomohiro; Takai, Madoka; Ishihara, Kazuhíko

doi:10.1021/la2037586

Cited by 50 publications

(41 citation statements)

References 27 publications

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“…The same cell responses were observed when other cells-that is, mouse fibroblast cells (L929 cells), [30,41] hepatocyte cells (HepG2 cells), [36] human cancer cells (HeLa cells), [38,42] and mouse embryonic stem cells (ES cells from 129/SvEv, passage 11) [31,46] -were encapsulated in the PMBV/PVA hydrogels.…”

Section: Application To Other Cell Systemssupporting

confidence: 57%

“…Relationship between the storage modulus of the PMBV/PVA hydrogel and the proliferation ratio of the encapsulated C3H10T1/2 cells after 3 days of culture. [38] Proliferation Cycle of Cells Encapsulated in the PMBV/PVA Hydrogel…”

Section: Cell Preservation In the Pmbv/ Pva Hydrogelmentioning

confidence: 99%

“…Fraction of C3H10T1/2 cells in the G1, S, and G2 phases after 1 and 3 days of culture on usual cell culture plates and in the PMBV/PVA hydrogels. [38] cultured on a usual cell culture plate. That is, the amount of albumin form HepG2 cells cultured on a conventional cell culture plate for 2 days was 150 AE 50 ng/10 5 -cells and decreased with culturing period.…”

Section: Application To Other Cell Systemsmentioning

confidence: 99%

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Bioinspired Phospholipid Polymer Hydrogel System for Cellular Engineering

Ishihara¹,

Oda

Aikawa

et al. 2015

Macromolecular Symposia

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Summary: The properties of the microenvironment surrounding cells are important for the control of cell functions. The polymeric cellular environment has great potential in this regard because environmental properties can be manipulated. We propose a spontaneously forming phospholipid polymer hydrogel system composed of 2 kinds of pre-polymers to control cellular functions via hydrogel physical properties. These pre-polymers are cytocompatible poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV) and water-soluble poly(vinyl alcohol) (PVA). The p-vinylphenylboronic acid units in PMBV can react with the hydroxy groups of PVA in aqueous medium and form crosslinkages. This spontaneously formed PMBV/PVA hydrogel can be dissociated again via the addition of sugar compounds. To alter the physical properties, we simply change the concentration or mixing ratio of the pre-polymers. The storage modulus of the PMBV/PVA hydrogel matrix was controlled from 0.3 kPa to 2.5 kPa, which corresponds to very soft natural tissue. Cells can be encapsulated in the hydrogel. When the storage modulus of the PMBV/PVA hydrogel was above 1.0 kPa, the proliferation of encapsulated cells was suppressed and provided uniform cells in G1 phase in cell cycle progression. High G1 phase fraction (>90%) may lead to excellent differentiation efficiency, which results in great insight for stem cell engineering. The PMBV/PVA system is expected to become a key material in cellular engineering for regulating cellular function without undesirable biological events.

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Section: Application To Other Cell Systemssupporting

confidence: 57%

Section: Cell Preservation In the Pmbv/ Pva Hydrogelmentioning

confidence: 99%

Section: Application To Other Cell Systemsmentioning

confidence: 99%

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Bioinspired Phospholipid Polymer Hydrogel System for Cellular Engineering

Ishihara¹,

Oda

Aikawa

et al. 2015

Macromolecular Symposia

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“…A new cell maintenance system called a 'cell-container' based on this hydrogel was proposed. By coupling the gelation system and microfluidic technique, encapsulation of a single cell into microgel beads was achieved by Aikawa et al [120]. Figure 13 outlines current applications of MPC polymers in biomedical and other fields.…”

Section: Cell-function Controllable Surfacesmentioning

confidence: 99%

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Iwasaki

Ishihara

2012

Science and Technology of Advanced Materials

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261

216

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This review article describes fundamental aspects of cell membrane-inspired phospholipid polymers and their usefulness in the development of medical devices. Since the early 1990s, polymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units have been considered in the preparation of biomaterials. MPC polymers can provide an artificial cell membrane structure at the surface and serve as excellent biointerfaces between artificial and biological systems. They have also been applied in the surface modification of some medical devices including long-term implantable artificial organs. An MPC polymer biointerface can suppress unfavorable biological reactions such as protein adsorption and cell adhesion -in other words, specific biomolecules immobilized on an MPC polymer surface retain their original functions. MPC polymers are also being increasingly used for creating biointerfaces with artificial cell membrane structures.

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“…Therefore, in order to further understand the relationship between cell cycle progression and the physical properties around the cells, PMBV/PVA hydrogel microspheres with varying physical properties were produced using a microfluidic channel system [51,52]. These microspheres allowed the encapsulation of single cells, eliminating the influence of cell-cell interactions.…”

Section: Spontaneously Forming Hydrogels Designed With Phospholipid Pmentioning

confidence: 99%