Sphingomyelins Prevent Propagation of Lipid Peroxidation—LC-MS/MS Evaluation of Inhibition Mechanisms

Coliva, Giulia; Lange, M.; Colombo, Simone; Chervet, Jean-Pierre; Domingues, Rosário M.; Fedorova, Maria

doi:10.3390/molecules25081925

Cited by 22 publications

(17 citation statements)

References 41 publications

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“…Furthermore, the chemical composition of the reactive species generated by the CPP depends on the distance of the plasma source from the target and the type of target [ 43 , 54 ]. Various methods have been developed to study the interaction of reactive species with lipid mono- and bilayers such as infrared reflection absorption spectroscopy (IRRAS) [ 55 ], atomic force microscopy [ 56 , 57 ], electrochemistry [ 58 , 59 , 60 ], and mass spectrometry [ 61 , 62 , 63 ]. Scholz et al introduced an electrochemical assay to quantify the oxidative damage of self-assembled monolayers (SAM) on gold electrodes [ 58 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

et al. 2021

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In the eye lens cell membrane, the lipid composition changes during the aging process: the proportion of sphingomyelins (SM) increases, that of phosphatidylcholines decreases. To investigate the protective role of the SMs in the lens cell membrane against oxidative damage, analytical techniques such as electrochemistry, high-resolution mass spectrometry (HR-MS), and atomic force microscopy (AFM) were applied. Supported lipid bilayers (SLB) were prepared to mimic the lens cell membrane with different fractions of PLPC/SM (PLPC: 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine). The SLBs were treated with cold physical plasma. A protective effect of 30% and 44% in the presence of 25%, and 75% SM in the bilayer was observed, respectively. PLPC and SM oxidation products were determined via HR-MS for SLBs after plasma treatment. The yield of fragments gradually decreased as the SM ratio increased. Topographic images obtained by AFM of PLPC-bilayers showed SLB degradation and pore formation after plasma treatment, no degradation was observed in PLPC/SM bilayers. The results of all techniques confirm the protective role of SM in the membrane against oxidative damage and support the idea that the SM content in lens cell membrane is increased during aging in the absence of effective antioxidant systems to protect the eye from oxidative damage and to prolong lens transparency.

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Section: Introductionmentioning

confidence: 99%

Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

et al. 2021

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“…It can inhibit lipid oxidation by the formation of an H-bond network within membranes [100]. Coliva et al (2020) suggested that sphingomyelins might prevent the propagation of lipid peroxidation [101]. According to Crnogaj et al (2015), B. canis infection in dogs is associated with a high concentration of lipid peroxidation [102].…”

Section: Discussionmentioning

confidence: 99%

Multi Platforms Strategies and Metabolomics Approaches for the Investigation of Comprehensive Metabolite Profile in Dogs with Babesia canis Infection

Rubić

Burchmore

Weidt

et al. 2022

IJMS

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Canine babesiosis is an important tick-borne disease worldwide, caused by parasites of the Babesia genus. Although the disease process primarily affects erythrocytes, it may also have multisystemic consequences. The goal of this study was to explore and characterize the serum metabolome, by identifying potential metabolites and metabolic pathways in dogs naturally infected with Babesia canis using liquid and gas chromatography coupled to mass spectrometry. The study included 12 dogs naturally infected with B. canis and 12 healthy dogs. By combining three different analytical platforms using untargeted and targeted approaches, 295 metabolites were detected. The untargeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) metabolomics approach identified 64 metabolites, the targeted UHPLC-MS/MS metabolomics approach identified 205 metabolites, and the GC-MS metabolomics approach identified 26 metabolites. Biological functions of differentially abundant metabolites indicate the involvement of various pathways in canine babesiosis including the following: glutathione metabolism; alanine, aspartate, and glutamate metabolism; glyoxylate and dicarboxylate metabolism; cysteine and methionine metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis. This study confirmed that host–pathogen interactions could be studied by metabolomics to assess chemical changes in the host, such that the differences in serum metabolome between dogs with B. canis infection and healthy dogs can be detected with liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) methods. Our study provides novel insight into pathophysiological mechanisms of B. canis infection.

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“…Changes in bioactive sphingolipids are a hallmark of metabolic chronic liver diseases, and the relationship between an increment of ceramide production, lipotoxicity, inflammation, and liver disease has been widely described 44 ; however, the increase in the SM(32:1) observed in the livers of relaxin-2-treated animals was not accompanied by an increase in ceramide hepatic levels, so, in our opinion, it should not be contemplated as an unfavorable effect. Indeed, SM are major components of mammalian cells with important and different physiological or pathological roles determined by the tissue context, by the composition/length of its acyl chains and by its concentration in the cells, 45 where they can also act as biophysical antioxidants 46 preventing lipid peroxidation, an arising central feature in hepatic lipotoxicity. 45 The CD36 receptor is a multifunctional membrane receptor that develops a crucial role in FA uptake and lipid metabolism and homeostasis, as well as in inflammation, and it directly participates in the rate of FA uptake by hepatocytes, being its hepatic expression significantly elevated in animal F I G U R E 4 Changes in the hepatic content of saturated and monounsaturated fatty acids, diglycerides and sphingomyelin in rats treated with relaxin-2.…”

Section: Discussionmentioning

confidence: 99%

Relaxin has beneficial effects on liver lipidome and metabolic enzymes

et al. 2021

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Relaxin is an insulin‐like hormone with pleiotropic protective effects in several organs, including the liver. We aimed to characterize its role in the control of hepatic metabolism in healthy rats. Sprague‐Dawley rats were treated with human recombinant relaxin‐2 for 2 weeks. The hepatic metabolic profile was analyzed using UHPLC‐MS platforms. Hepatic gene expression of key enzymes of desaturation (Fads1/Fads2) of n‐6 and n‐3 polyunsaturated fatty acids (PUFAs), of phosphatidylethanolamine (PE) N‐methyltransferase (Pemt), of fatty acid translocase Cd36, and of glucose‐6‐phosphate isomerase (Gpi) were quantified by Real Time‐PCR. Activation of 5′AMP‐activated protein kinase (AMPK) was analyzed by Western Blot. Relaxin‐2 significantly modified the hepatic levels of 19 glycerophospholipids, 2 saturated (SFA) and 1 monounsaturated (MUFA) fatty acids (FA), 3 diglycerides, 1 sphingomyelin, 2 aminoacids, 5 nucleosides, 2 nucleotides, 1 carboxylic acid, 1 redox electron carrier, and 1 vitamin. The most noteworthy changes corresponded to the substantially decreased lysoglycerophospholipids, and to the clearly increased FA (16:1n‐7/16:0) and MUFA + PUFA/SFA ratios, suggesting enhanced desaturase activity. Hepatic gene expression of Fads1, Fads2, and Pemt, which mediates lipid balance and liver health, was increased by relaxin‐2, while mRNA levels of the main regulator of hepatic FA uptake Cd36, and of the essential glycolysis enzyme Gpi, were decreased. Relaxin‐2 augmented the hepatic activation of the hepatoprotector and master regulator of energy homeostasis AMPK. Relaxin‐2 treatment also rised FADS1, FADS2, and PEMT gene expression in cultured Hep G2 cells. Our results bring to light the hepatic metabolic features stimulated by relaxin, a promising hepatoprotective molecule.

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Sphingomyelins Prevent Propagation of Lipid Peroxidation—LC-MS/MS Evaluation of Inhibition Mechanisms

Cited by 22 publications

References 41 publications

Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress

Multi Platforms Strategies and Metabolomics Approaches for the Investigation of Comprehensive Metabolite Profile in Dogs with Babesia canis Infection

Relaxin has beneficial effects on liver lipidome and metabolic enzymes

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