Sphingosinicella microcystinivorans gen. nov., sp. nov., a microcystin-degrading bacterium

Abstract

Three strains of bacteria that degrade the cyanobacterial hepatotoxin microcystin, Y2 T , MDB2 and MDB3, were isolated from a eutrophic lake, Lake Suwa, and the Tenryu River, Japan, and characterized. These strains were aerobic and chemo-organotrophic and their cells were Gram-negative, non-spore-forming rods, motile by means of single polar flagella. Yellow-pigmented colonies were formed on nutrient agar media. The strains assimilated only citrate among the organic compounds tested as carbon sources. The G+C content of genomic DNA ranged from 63?6 to 63?7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolates formed a tight cluster within the family Sphingomonadaceae but were clearly separate from established genera of this family, e.g. Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis; sequence similarities between the new isolates and type strains from established genera ranged from 90?9 to 94?9 %. Chemotaxonomic and phenotypic data supported the conclusion that these strains were members of the family Sphingomonadaceae. The major components of the cellular fatty acids were 18 : 1v7c (36-41 %) and 16 : 1v7c (33-36 %). Hydroxy fatty acids were mainly 2-OH 14 : 0 (11-13 %), and 3-OH fatty acids were absent. Glycosphingolipids were detected. Ubiquinone-10 and homospermidine were present as the major quinine and polyamine, respectively. Thus, it is proposed that the three strains represent a new genus and species of the family Sphingomonadaceae with the name Sphingosinicella microcystinivorans gen. nov., sp. nov. The type strain is Y2 T (=KCTC 12019 T =JCM 13185 T ).

Sphingomonas ginsengisoli sp. nov. and Sphingomonas sediminicola sp. nov.

“…S1). The major polyamine of both novel strains, like that of all established members of the genus Sphingomonas sensu stricto (Maruyama et al, 2006;Takeuchi et al, 2001;Hamana et al, 2003), was homospermidine. As shown in Table 2, the major fatty acids of both strains included summed feature 7 (C 18 : 1 v7c, C 18 : 1 v9t and/or C 18 : 1 v12t), C 16 : 0 and C 17 : 1 v6c, which are consistent with major fatty acids of other related species of the genus Sphingomonas.…”

Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively

“…Among the strains tested, strains 5413J-26 T , KIS18-15 T , Sphingomonas paucimobilis KACC 10931 T and Sphingomonas trueperi KACC 12329 T showed similar fatty acid profiles with small amounts of C 17 : 1 v6c and C 17 : 1 v8c, compared to that of Sphingomonas soli KACC 13009 T (Table 2). Sphingomonas soli KACC 13009 T had a fatty acid composition similar to that of Sphingomonas jaspsi and Sphingomonas sediminicola (An et al, 2013).The major polyamine of both the novel strains was homospermidine, which is present in all members of the genus Sphingomonas with validly published names (Maruyama et al, 2006;Takeuchi et al, 2001;Hamana et al, 2003 …”

Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea

“…The genus Sphingosinicella was proposed by Maruyama et al (2006) with the description of a single species, Sphingosinicella microcystinivorans, as a novel member of the family Sphingomonadaceae. Subsequently, another Sphingosinicella species, Sphingosinicella xenopeptidilytica, was described (Geueke et al, 2007).…”