Spider Silk – a Versatile Biomaterial for Tissue Engineering and Medical Applications

Strauß, Sarah; Reimers, Kerstin; Allmeling, Christina; Kuhbier, Joern W.; Radtke, Christine; Schäfer-Nolte, Franziska; Wendt, Hanna; Vogt, Peter M.

doi:10.1515/bmt-2013-4068

Cited by 6 publications

(7 citation statements)

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“…However, current synthetic models are unable to actively respond to cellular signals and often lack the ability to host cell attachment or growth 3,4 . Biologic scaffolds are typically made from purified proteins such as collagen, fibrin, silk, or gelatin 5‐8 . Although these scaffolds have been shown to promote cell adhesion and remodeling, they often lack the mechanical properties needed for a functional vessel 9,10 .…”

Section: Introductionmentioning

confidence: 99%

“…typically made from purified proteins such as collagen, fibrin, silk, or gelatin. [5][6][7][8] Although these scaffolds have been shown to promote cell adhesion and remodeling, they often lack the mechanical properties needed for a functional vessel. 9,10 To address the aforementioned challenges, the use of hybrid scaffolds, made from proteins present in the extracellular matrix (ECM) and synthetic materials, have been widely studied.…”

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confidence: 99%

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Re‐endothelialization of non‐detergent decellularized porcine vessels

et al. 2020

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The optimal vascular graft should exhibit sufficient mechanical strength, low immunogenicity, high biocompatibility, and resistance to calcification, thrombosis, stenosis, and infection. 1 From a surgical perspective, it should be easy to handle, have reasonable manufacturing costs, and be readily available. Large diameter vascular grafts (>5 mm luminal diameter) have been successfully developed, but smaller vascular grafts (<5 mm luminal diameter) are difficult to generate because of blood clotting, scarring, or occlusion after implantation. 1 The materials used to construct vascular grafts fall into two categories: synthetic or biologic. 2 Synthetic scaffolds are either made from nondegradable polymers such as polytetrafluoroethylene and Dacron, which are widely used in the clinic, or biodegradable polymers such as poly(lactic-acid) and poly(glycolic-acid). 1,2 Synthetic scaffolds are advantageous with respect to the manufacturing process providing reproducible properties such as mechanical strength, size, and configuration. 1 However, current synthetic models are unable to actively respond to cellular signals and often lack the ability to host cell attachment or growth. 3,4 Biologic scaffolds are

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Re‐endothelialization of non‐detergent decellularized porcine vessels

et al. 2020

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“…Spider silk from the golden orb web spider Nephila edulis combines a variety of properties which make it an attractive material for various technical and medical applications. 24 These include its elasticity and breaking strength in combination with its extremely light weight and shape memory properties 25 in addition to its remarkable bio- and hemocompatibility, which has been well documented in different in vitro and in vivo studies. 24,26 Spider silk consists predominantly of large proteins whose non-repetitive C and N terminus enclose a highly repetitive central part.…”

Section: Discussionmentioning

confidence: 99%

“…24 These include its elasticity and breaking strength in combination with its extremely light weight and shape memory properties 25 in addition to its remarkable bio- and hemocompatibility, which has been well documented in different in vitro and in vivo studies. 24,26 Spider silk consists predominantly of large proteins whose non-repetitive C and N terminus enclose a highly repetitive central part. These proteins undergo a change in conformity in the spinneret, which leads to the formation of fibrils with pseudocrystalline regions embedded in an amorphous matrix and thus increases the strength of the spider thread.…”

Section: Discussionmentioning

confidence: 99%

Pressure-compacted and spider silk–reinforced fibrin demonstrates sufficient biomechanical stability as cardiac patch in vitro

et al. 2021

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Objective: The generation of bio-/hemocompatible cardiovascular patches with sufficient stability and regenerative potential remains an unmet goal. Thus, the aim of this study was the generation and in vitro biomechanical evaluation of a novel cardiovascular patch composed of pressure-compacted fibrin with embedded spider silk cocoons. Methods: Fibrin-based patches were cast in a customized circular mold. One cocoon of Nephila odulis spider silk was embedded per patch during the casting process. After polymerization, the fibrin clot was compacted by 2 kg weight for 30 min resulting in thickness reduction from up to 2 cm to <1 mm. Tensile strength and burst pressure was determined after 0 weeks and 14 weeks of storage. A sewing strength test and a long-term load test were performed using a customized device to exert physiological pulsatile stretching of a silicon surface on which the patch had been sutured. Results: Fibrin patches resisted supraphysiological pressures of well over 2000 mmHg. Embedding of spider silk increased tensile force 1.8-fold and tensile strength 1.45-fold ( p < .001), resulting in a final strength of 1.07 MPa and increased sewing strength. Storage for 14 weeks decreased tensile strength, but not significantly and suturing properties of the spider silk patches were satisfactory. The long-term load test indicated that the patches were stable for 4 weeks although slight reduction in patch material was observed. Conclusion: The combination of compacted fibrin matrices and spider silk cocoons may represent a feasible concept to generate stable and biocompatible cardiovascular patches with regenerative potential.

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“…The application of spider silk as a guiding structure for nerve regeneration is an upcoming innovation. Making use of spider silk is an idea dating back thousands of years [32]. For example, it is known that cobwebs were used to stop bleeding in ancient Rome, and natives of the Salomon Islands have been using spider silk-woven fishing lines for the last 300 years to catch small fish from their boats [33,34].…”

Section: Testing the Hypothesismentioning

confidence: 99%

Ex vivo limb perfusion for traumatic amputation in military medicine

Kaltenborn

Krezdorn

Hoffmann³

et al. 2020

Military Med Res

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Background: Limb loss has a drastic impact on a patient's life. Severe trauma to the extremities is common in current military conflicts. Among other aspects, "life before limb" damage control surgery hinders immediate replantation within the short post-traumatic timeframe, which is limited in part by the ischemic time for successful replantation. Ex vivo limb perfusion is currently being researched in animal models and shows promising results for its application in human limb replantation and allotransplantation.

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Spider Silk – a Versatile Biomaterial for Tissue Engineering and Medical Applications

Cited by 6 publications

References 0 publications

Re‐endothelialization of non‐detergent decellularized porcine vessels

Re‐endothelialization of non‐detergent decellularized porcine vessels

Pressure-compacted and spider silk–reinforced fibrin demonstrates sufficient biomechanical stability as cardiac patch in vitro

Ex vivo limb perfusion for traumatic amputation in military medicine

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