Spleen immune status is affected after acute handling stress but not regulated by cortisol in Eurasian perch, Perca fluviatilis

Physiological and proteomic evidences that domestication process differentially modulates the immune status of juvenile Eurasian perch (Perca fluviatilis) under chronic confinement stress

“…Respiratory burst activity, estimated by reactive oxygen species (ROS) production, was evaluated following the nitroblue tetrazolium protocol used by Milla et al [8]. Spleen-Somatic Index (SSI) was calculated as spleen weight (g)/body weight (g) * 100.…”

“…Deleterious effects on growth and reproductive function as well as on immune system and disease resistance have already been reported when fish encountered chronic or repeated stressors [1e6]. The energetic demand associated with the stress response may reduce the ability of the organism to maintain normal physiological functions, including immunity [1e4, 7,8]. Such a detrimental modulation of the defense capacity predisposes captive individuals to infectious diseases and is damageable since many pathogens are opportunistic [5].…”

Імуноферментний Метод Визначення Лізоциму Слизу Та Тканин Риб

“…ХХ ст., коли для визначення лізоциму в біологічному матеріалі переважно використовували турбидиметричний метод, який базується на визначенні зменшення ступеня мутності суспензії тест-бактерій M. lysodeicticus (M. luteus) під дією лізоциму в певний інтервал часу (Lukianenko,1971;Labinskaya, 1978;Mikryakov, 1978;Vladimirov, 1982;Vovk et al, 1997;Metodicheskiye ukazaniya...,1987;Ellis, 1990). Турбидиметричний метод застосовують при вивченні організму риб і на даний час (Milla et al, 2010;Garth LFletcher et al, 2011). Використання високочутливих сучасних методів досліджень дасть можливість уточнити та доповнити знання з імунобіології риб.…”

The trenbolone acetate affects the immune system in rainbow trout, Oncorhynchus mykiss

“…The ACH50 was evaluated following the method adapted by Milla et al (2010). To sum up, the plasma, liver and kidney lysates were serially diluted in veronal buffer (Biomérieux, Marcy l'Etoile, France) in a U-bottom 96-well plates (1/40 to 1/800 for the plasma, 1/5 to 1/107 for the liver and 1/1 to 1/512 for the kidney).…”

“…The total immunoglobulin concentrations in liver were measured following a method adapted from Milla et al (2010). In a few words, immunoglobulins were precipitated with 10,000 kDa polyethylene glycol (PEG, Sigma).…”