Splenic function and red blood cell deformability: The beneficial effects of spleen autotransplantation in animal experiments

Abstract

Erythrocyte deformability could be a suitable parameter reflecting hyposplenic-asplenic conditions after splenectomy or spleen autotransplantation. The aim of this study was to analyze the efficacy of spleen autotransplantation using filtrometry and ektacytometry for testing erythrocyte deformability, besides complex structural, functional, hematological, hemostaseological and immunological examinations.Healthy beagle dogs were subjected to the study in the following experimental groups: "Sham"-sham operated control, "SE"splenectomy, "AU-5" and "AU-10"-autotransplantation with 5 or 10 spleen chips using "Furka's spleen chip" technique after splenectomy. Blood samples were taken before the operations and monthly afterwards for 1 year and bi-monthly till the end of the 2nd postoperative year. Erythrocyte deformability was measured by bulk filtrometry (relative cell transit time, RCTT) and on the 20th-24th postoperative months by ektacytometry (elongation index, EI), too.RCTT was elevated in operated groups till the 12th month. On the 20th/24th months "SE"-group expressed the highest level, while the "AU"-groups were close to "Sham"-group. Using ektacytometer we found that on the 20th/24th month both EImax and SS1/2 showed the lowest values (because of unusual curve characteristic) in "SE"-group and "AU"-groups almost reached the "SH"-group.Spleen autotransplantation partially restored the normal rheological conditions. Both filtrometry and ektacytometry was suitable to investigate hyposplenic-asplenic conditions.

Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation

“…It is well known that rigid red blood cells besides other particles are removed from the circulation normally by the spleen. Consequently, decrease in splenic filtration function may results in hemorheological changes [2,20,21,29]. Impaired rheological properties of the blood lead microcirculatory disturbances [2,17,28,33].…”

“…We used this relatively simple measuring method on beagle dogs, as well. Later, by developing our laboratory equipments, we had the opportunity to test deformability by ektacytometry as well (RheoScan D-200) presenting novel data in a beagle animal model [21]. In the latest experimental series we could apply rotational ektacytometry measurement (LoRRca MaxSis Osmoscan).…”

Neuroprotective Effect of Hydrogen-Rich Saline in Global Cerebral Ischemia/Reperfusion Rats: Up-Regulated Tregs and Down-Regulated miR-21, miR-210 and NF-κB Expression

“…Therefore, it is vitally important to maintain the immune balance to reduce brain damage. The spleen is an important immune organ that consists of aggregates of lymphoid tissue [ 41 ], and therefore was chosen for analysis of representative immunological functions in this study. Regulatory T cells (Tregs) are antigen specific, possessing T-cell receptors, and they possess a suppressive function.…”

Protective effect of hydrogen‐rich saline against radiation‐induced immune dysfunction

“…), inflammatory diseases and even cancer . As one of the most important immune organs, the immunological function of spleen is mainly carried out by white pulp, which consists of aggregates of lymphoid tissue . The injury of spleen can cause dysfunction of immune system and make one susceptible to infections and other diseases.…”