Spontaneous mouse mammary tumours: incidence and cytokeratin expression

García‐Iglesias, M. J.; Pérez‐Martínez, Claudia; Bravo-Moral, A M; Ferreras, María del Carmen; García‐Fernández, Rosa A.; Espinosa-Alvarez, J.; Escudero-Díez, A.

doi:10.1016/s0034-5288(97)90164-8

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…30,68 Vet Pathol 43 :1, 2006 Keratin 6 is typically associated with epidermal proliferation and is expressed in the inner layer of the outer root sheath, hyperplasic epidermis, conjunctiva, oral mucosa, and some preneoplastic and neoplastic mouse mammary lesions. 19,27,28,49,57,60,72 On the basis of the pattern of distribution of K6 in stratified epithelia, this keratin should not be expressed in the normal mammary gland, which we confirmed at the RNA level. Similarly, our results indicated that K13 is not expressed in the mouse mammary gland, whereas this keratin has been reported to be expressed in the human mammary gland.…”

Section: Other Immunohistochemical Markerssupporting

confidence: 65%

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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Abstract. Immunohistochemical analysis using paraffin-embedded specimens is the method of choice to evaluate protein expression at a cellular level while preserving tissue architecture in normal and neoplastic tissues. Current knowledge of the expression of terminal differentiation markers in the mouse mammary gland relies on the evaluation of frozen tissues by use of immunofluorescence. We assessed changes in patterns of expression of terminal differentiation markers throughout the development of the mouse mammary gland in paraffin-embedded tissues. The expression of a-smooth muscle actin (SMA) and keratins (K) 5, 8/18, and 14 was influenced by the development stage of the mammary gland. Expression of K5 and SMA was restricted to basal cells. Keratin 14 was consistently expressed by mammary basal cells, and was detected in scattered luminal cells from 13.5 days after conception through puberty. Labeling for K8/18 of luminal cells was heterogeneous at all times. Heterogeneous expression patterns in luminal cells suggest this layer has cells with a variety of biological functions. The absence of K6 expression at any stage of the development of the mammary gland was confirmed by use of reverse transcriptase-polymerase chain reaction analysis, which indicates that this intermediate filament is not a marker of the mammary gland stem cell. Finally, consistent with results of earlier studies, keratins 1, 10, 13, and 15, and filaggrin, involucrin, and loricrin were not detected at any stage of mammary gland development.

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Section: Other Immunohistochemical Markerssupporting

confidence: 65%

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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“…In the current research, the pattern of reaction of CK 7 in epithelial cells of GP mammary gland and tumors is similar to those described in other species. 11,15,39,40 Mammary basal/ myoepithelial cells in the present study were also positive for CK 7, whereas human, feline, canine, and mice mammary basal/myoepithelial cells do not express this CK, 11,15,39,40 although the expression of CK 7 by cells with muscle differentiation has been reported in human beings. 26,40 The expression of CK 20 and CK 7 and the lack of expression of CK 8 in GP mammary gland tissue seem to represent a significant difference with respect to the CK profiles of other mammalian species.…”

Section: Discussionsupporting

confidence: 44%

Morphological and Immunohistochemical Characterization of Spontaneous Mammary Gland Tumors in the Guinea Pig (Cavia porcellus)

et al. 2009

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Ten spontaneous mammary gland tumors affecting guinea pigs (GP) were analyzed histologically and immunohistochemically. Histologically, 3 were benign (2 simple adenomas and 1 benign mixed tumor) and 7 were malignant (1 simple solid carcinoma and 6 simple tubulopapillary carcinomas). Immunohistochemical data revealed the glandular immunoprofile of all the tumors and suggested their ductal origin on the basis of cytokeratin 20 expression. Interestingly, cytokeratin 7 was detected in basal/ myoepithelial cells. Further, all tumors were positive for type a estrogen and progesterone receptors, suggesting a role for steroid hormones in the development of these neoplasias in GP. This article describes the morphological and immunohistochemical features of the normal mammary gland and spontaneous mammary gland tumors in GP.

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“…(Moulton, 1990;Yager and Scott, 1993). Mammary gland tumors have been even less frequently reported in nondomestic animals (Bhowmik and Iyer, 1978;Carpenter et al, 1980;Schmidt et al, 1986;Hotchkiss, 1995;Harrenstien et al, 1996;Garcia-Iglesias et al, 1997). We believe this is the first report of mammary gland tumors in African hedgehogs.…”

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confidence: 79%

Mammary Gland Tumors in Captive African Hedgehogs

Raymond

Garner

2000

Journal of Wildlife Diseases

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From December 1995 to July 1999, eight mammary gland tumors were diagnosed in eight adult captive female African hedgehogs (Atelerix albiventris). The tumors presented as single or multiple subcutaneous masses along the cranial or caudal abdomen that varied in size for each hedgehog. Histologically, seven of eight (88%) mammary gland tumors were malignant. Tumors were classified as solid (4 cases), tubular (2 cases), and papillary (2 cases). Seven tumors had infiltrated into the surrounding stroma and three tumors had histologic evidence of neoplastic vascular invasion. Three hedgehogs had concurrent neoplasms. These are believed to be the first reported cases of mammary gland tumors in African hedgehogs.

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Spontaneous mouse mammary tumours: incidence and cytokeratin expression

Cited by 6 publications

References 9 publications

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Morphological and Immunohistochemical Characterization of Spontaneous Mammary Gland Tumors in the Guinea Pig (Cavia porcellus)

Mammary Gland Tumors in Captive African Hedgehogs

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