Spontaneous reanastomosis between lymphatic vessels following syngeneic transplantation of the small intestine in the rat

Üner, Atilla; Weinberg, Annelie M.; Nautrup, Cordula Poulsen; Kassianoff, I.; Lüdemann, Wolf; Schier, Felix; Claus, Piet; Rautenfeld, D. Berens von

doi:10.1007/s00276-001-0383-5

Cited by 11 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the newly formed capillary lymphatic network at the replantation level did not connect with any pre-existing similar network. These findings are in agreement with those of others [4,18], who reported a newly formed lymphatic network at the replantation level that was not accompanied by a major lymphatic vessel and which differed morphologically and functionally from the original network [5]. However, others [1,2] believe in the presence of pre-existing lymphatics in super-ficial tissues that are activated after the injury, connected with the newly created lymphatics, and which play an important role in collateral lymph circulation.…”

Section: Discussionsupporting

confidence: 92%

Lymphatic Regeneration Following Hind Limb Replantation: An Experimental Study in the Dog

Smaropoulos

Papazoglou²,

Patsikas³

et al. 2005

Eur J Pediatr Surg

View full text Add to dashboard Cite

Different types of trauma to the lymphatic system can often occur, but surgical intervention can be performed only in specific cases. We report on lymphatic regeneration following limb replantation in traumatic amputations and replantation of extremities. The aim of this study was to observe the progression and reaction after surgical trauma that is similar to other kinds of trauma, both in children or adults, and to monitor the possible lymphatic regeneration. Particular attention was paid to two parameters: firstly, the physical examination of the replanted limbs by checking the post-traumatic lymphoedema, and secondly, the study of the images taken from indirect lymphangiography of the replanted limbs. Histological specimens of the surgical trauma area were also examined to reconfirm or exclude lymphatic regeneration. The study population consisted of sixteen mongrel dogs, divided into two groups of eight animals each, who underwent hind limb elective amputation and replantation combined with (group A) or without (group B) sciatic nerve division. Lymphoedema formation was followed quantitatively by measurement of the circumference of the hind limb for 21 days after replantation. Indirect lymphography, never performed before in such cases, and histopathology, were performed to evaluate and confirm lymphatic regeneration. Lymphatic regeneration after replantation of the operated hind limbs was first confirmed between 7th and 11th postoperative day by indirect lymphangiography and clinical observation of the post-traumatic lymphoedema of these limbs. The mean time of visualisation of lymphatic regeneration through lymphography was 10.12 days for group A and 9.37 days for group B. However, nerve transection had no effect on lymphatic regeneration (p = 0.46). Histopathological examination showed first evidence of lymphatic regeneration on the ninth postoperative day and a network of newly formed capillary lymphatics on the 21st postoperative day. It is concluded that lymphatic regeneration following replantation of the extremities without anastomosing of the interrupted lymph vessels, is an unquestionable fact. To achieve the best lymphatic drainage and use of the replanted extremities it is important to resect all non-vital tissues of the replantation area. Local or general infections decelerate lymphatic regeneration. Indirect lymphography with iotrolan is a reliable, easy to perform technique without complications that may be used repeatedly for confirmation and evaluation of post-traumatic lymphoedema.

show abstract

Section: Discussionsupporting

confidence: 92%

Lymphatic Regeneration Following Hind Limb Replantation: An Experimental Study in the Dog

Smaropoulos

Papazoglou²,

Patsikas³

et al. 2005

Eur J Pediatr Surg

View full text Add to dashboard Cite

show abstract

“…Therefore, re-anastomosis of blood vessels brings the accompanying lymphatic vessels into close contact, which is obviously sufficient for their rapid functional restoration. Since the lymphatic system is made up of a network, flow of the lymph after organ transplantation can take different routes than the original ones, and lympho-lymphatic anastomoses may form at unusual places [3]. From the stump of a dissected collector numerous sprouts of small calibre are formed, on the basis of increased proliferation of LECs at the terminal portion of the damaged vessel [4].…”

Section: Regenerationmentioning

confidence: 99%

Development of Lymphatic Vessels: Tumour Lymphangiogenesis and Lymphatic Invasion

Wilting

Hawighorst²,

Hecht³

et al. 2005

CMC

View full text Add to dashboard Cite

In human solid cancer, the lymph node status is the most important prognostic indicator for the clinical outcome of patients. Follow-up data has shown that about 80% of metastasis follows an orderly pattern of progression via the lymphatic network while about 20% systemic metastasis occurs, bypassing the lymphatic system. Over the past few years, advances have been made in understanding the cellular and molecular aspects of physiological lymphangiogenesis and tumour-induced lymphangiogenesis, and the majority of studies point out to a positive correlation between tumour-induced lymphangiogenesis and lymphatic metastasis. However, the impact of intra- and peritumoural lymphatics on the tumour biology and the first steps of lymphatic metastasis, i.e. the invasion of tumour cells into the lymphatic vessels, are not well understood. We will give an outline of i. the physiological process of lymphangiogenesis, ii. tumour-induced lymphangiogenesis and lymphatic metastasis, iii. lymphatic invasion and the common pathways of tumour-lymphangiogenesis and lymphatic invasion. The growing interest in this topic has brought up a number of new molecular players in the field, which may provide the basis for a rational therapy against the process of lymphatic dissemination of tumour cells.

show abstract

“…Previous authors (Ikomi et al, 2006) showed that interrupted lymphatic collectors can regrow and reconnect over a limited distance only. Uner et al (2001) demonstrated the regrowth of capillaries out of lymph vascular stumps after wounding. Therefore, we expected to see the lymphatic capillaries of scars in our preparations filled with Berlin Blue, but we did not.…”

Section: Discussionmentioning

confidence: 99%

Anatomy of the Subcutaneous Lymph Vascular Network of the Human Leg in Relation to the Great Saphenous Vein

Schacht

Luedemann

Abels

et al. 2008

The Anatomical Record

View full text Add to dashboard Cite

The anatomical relationship between lymphatic collectors and veins is of clinical importance for preventing lymphedema secondary to lymphatic collector injury during surgical procedures. To identify areas at risk during surgical interventions, we performed an anatomical study of human legs. The lymphatic collectors of 42 legs of human cadavers were injected with Berlin Blue solution or contrast medium. After fixation, the collectors were dissected and their distances from the great saphenous vein were determined. We found that the lymphatic collectors on the dorsum of the foot ran in close parallel with the corium, whereas in the groin a greater number of lymphatic collectors clustered around the great saphenous vein. The ventromedial bundle that drains into the superficial inguinal nodes included 5-20 lymphatic collectors. The average width of the ventromedial bundle varied between 116 mm at the middle of the lower leg and 32 mm at the groin. Our study cannot confirm the previous observation of a bottleneck of the ventromedial bundle occurring at the knee, but does support the finding of an elongated bottleneck at the thigh and groin draining into the superficial inguinal lymph nodes. In addition, the idea of one sentinel lymph node for a specific region of the leg is not supported by these data. These observations will help surgeons to plan incisions and dissections with respect to lymphatic collectors, thereby minimizing damage to them and reducing complications resulting from unnecessary lymphatic excisions. Anat Rec, 292:87-93, 2009Rec, 292:87-93, . 2008 Wiley-Liss, Inc.Key words: lower limb lymphatics; lower limb veins; lymphedema; microcirculationThe lymph vascular system begins with blind ending lymphatic capillaries and transports the lymphatic fluid via precollectors, collectors, the cysterna chyli, and thoracic duct into the blood vascular system. The lymphatic system of the extremities consists of a network of subfascial intermuscular and subcutaneous lymphatic collectors connected by perforating lymphatic collectors that provide lymphatic flow from deep anatomical regions to the surface (Földi and Földi, 2003). The perforating lymphatic collectors accompany the perforating veins through the muscle fascia. The epifascial lymphatic system of the human leg is divided into the ventromedial and dorsolateral lymphatic bundle, depending

show abstract

Spontaneous reanastomosis between lymphatic vessels following syngeneic transplantation of the small intestine in the rat

Cited by 11 publications

References 12 publications

Lymphatic Regeneration Following Hind Limb Replantation: An Experimental Study in the Dog

Lymphatic Regeneration Following Hind Limb Replantation: An Experimental Study in the Dog

Development of Lymphatic Vessels: Tumour Lymphangiogenesis and Lymphatic Invasion

Anatomy of the Subcutaneous Lymph Vascular Network of the Human Leg in Relation to the Great Saphenous Vein

Contact Info

Product

Resources

About