Spore inoculation of
 Bacillus pumilus
 TUAT1 strain, a biofertilizer microorganism, enhances seedling growth by promoting root system development in rice

Ngo, Ngoc Phuong; Yamada, Tetsuya; Higuma, Shungo; Ueno, Naoya; Saito, Kaya; Kojima, Katsuhiro; Maeda, Masumi; Yamaya-Ito, Hiroko; Ohkama‐Ohtsu, Naoko; Kanekatsu, Motoki; Yokoyama, Tadashi

doi:10.1080/00380768.2019.1689795

Cited by 12 publications

(17 citation statements)

References 29 publications

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“…The inoculation efficiency of B. pumilus was studied in various conditions including in vitro , growth chambers, greenhouses, and in-field conditions. A lot of these studies focus on rice growth promotion and deal mainly with biometric parameters and chemical properties of shoots and roots ( Win et al, 2018 ; Ngo et al, 2019 ; Liu et al, 2020 ) Importantly, both commercial strains and soil isolates are used to study on the effects of bacteria on the efficiency in promoting plant growth.…”

Section: Introductionmentioning

confidence: 99%

Potential of Bacillus pumilus to directly promote plant growth

2022

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Plant Growth-Promoting Bacteria (PGPB) are a promising alternative to conventional fertilization. One of the most interesting PGPB strains, among the spore-forming bacteria of the phylum Firmicutes, is Bacillus pumilus. It is a bacterial species that inhabits a wide range of environments and shows resistance to abiotic stresses. So far, several PGPB strains of B. pumilus have been described, including B. pumilus LZP02, B. pumilus JPVS11, B. pumilus TUAT-1, B. pumilus TRS-3, and B. pumilus EU927414. These strains have been shown to produce a wide range of phytohormones and other plant growth-promoting substances. Therefore, they can affect various plant properties, including biometric traits, substance content (amino acids, proteins, fatty acids), and oxidative enzymes. Importantly, based on a study with B. pumilus WP8, it can be concluded that this bacterial species stimulates plant growth when the native microbiota of the inoculated soil is altered. However, there is still a lack of research with deeper insights into the structure of the native microbial community (after B. pumilus application), which would provide a better understanding of the functioning of this bacterial species in the soil and thus increase its effectiveness in promoting plant growth.

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Section: Introductionmentioning

confidence: 99%

Potential of Bacillus pumilus to directly promote plant growth

2022

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“…3 H). Ngo et al (2019) reported that the inoculation of rice with viable spores increased the number and density of lateral root, while that with viable vegetative cells did not. Furthermore, the inoculation with viable spores suppressed average lateral root length, whereas that with vegetative cells increased mean lateral root length.…”

Section: Resultsmentioning

confidence: 99%

“… Ngo et al (2019) suggested that an inoculation with B. pumilus TUAT1 spores promoted crown root formation and elongation by enhancing the production of auxin ( Ngo et al , 2019 ). The elongation of crown roots and enhanced crown root differentiation were the most strongly affected by the VS inoculation ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The inoculation with autoclaved dead spores also increased the plant N content, which may also be attributed, at least in part, to its effects on root parameters. Ngo et al (2019) suggested that an inoculation with B. pumilus TUAT1 spores promoted crown root formation and elongation by enhancing the production of auxin (Ngo et al, 2019). The elongation of crown roots and enhanced crown root differentiation were the most strongly affected by the VS inoculation (Fig.…”

Section: Autoclaved Dead Spores Promote S Viridis Plant Growthmentioning

confidence: 93%

“…The whole genome of this bacterium was sequenced by Okazaki et al (2019) , revealing the presence of genes for the production of indole-3-acetic acid (IAA), siderophores, and acetoin, which have been shown to promote plant growth and development. Ngo et al (2019) demonstrated that an inoculation with B. pumilus TUAT1 spore cells promoted rice growth by increasing plant height, root weight, and shoot weight, whereas that with vegetative cells only altered plant height. Furthermore, an inoculation with the autoclaved, dead spore cells of Bacillus strains, such as B. pumilus TUAT1, JM52, MAFF118530, and MAFF301706, B. altitudinis JR4 and JR198, and B. megaterium MAFF301694 and MAFF520023 promoted rice growth even though their abilities to produce IAA, fix nitrogen, or solubilize phosphorous differed.…”

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confidence: 98%

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Plant Growth-promoting Effects of Viable and Dead Spores of Bacillus pumilus TUAT1 on Setaria viridis

et al. 2022

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Spores are a stress-resistant form of Bacillus spp., which include species that are plant growth-promoting rhizobacteria (PGPR). Previous studies showed that the inoculation of plants with vegetative cells or spores exerted different plant growth-promoting effects. To elucidate the spore-specific mechanism, we compared the effects of viable vegetative cells, autoclaved dead spores, and viable spores of Bacillus pumilus TUAT1 inoculated at 10 7 CFU plant -1 on the growth of the C4 model plant, Setaria viridis A10.1. B. pumilus TUAT1 spores exerted stronger growth-promoting effects on Setaria than on control plants 14 days after the inoculation. Viable spores increased shoot weight, root weight, shoot length, root length, and nitrogen uptake efficiency 21 days after the inoculation. These increases involved primary and crown root formation. Additionally, autoclaved dead spores inoculated at 10 8 or 10 9 CFU plant -1 had a positive impact on crown root differentiation, which increased total lateral root length, resulting in a greater biomass and more efficient nitrogen uptake. The present results indicate that an inoculation with viable spores of B. pumilus TUAT1 is more effective at enhancing the growth of Setaria than that with vegetative cells. The plant response to dead spores suggests that the spore-specific plant growth-promoting mechanism is at least partly independent of symbiotic colonization.

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Seed coating by biofertilizer containing spores of Bacillus pumilus TUAT1 strain enhanced initial growth of Oryza sativa L.

Agake

Ramírez

Kojima³

et al. 2021

Agronomy Journal

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In rice production, early growth is often slow in direct‐seeded system. Techniques to overcome this barrier are needed. This study evaluated the effectiveness of Kikuichi—a biofertilizer containing Bacillus pumilus TUAT1 spore—in promoting rice growth in a direct‐sowing system. Oxygen, iron, and molybdenum as coating materials were also evaluated to combine with biofertilizer. The seeds were coated with powdered Kikuichi with polyvinyl alcohol followed by a coating with calcium peroxide (CALPER), molybdenum (Benmoly), and iron powder. Double coating with the biofertilizer and Benmoly promoted plant growth. We applied amounts of Kikuichi equivalent to one, two and a half, and five times the weight of the seeds and found that the growth‐promoting effect was dependent on the amount applied. The best timing of application of the coating, specifically during sprouting, was before the coleoptile emerged. Bacillus pumilus TUAT1 can enhance the growth of rice when it exists in high concentration on the spermosphere by coating with molybdenum, but double coating with neither calcium peroxide nor iron powder contributed to the initial growth of the seeds. Kikuichi coating with Benmoly has positive effects on the rice growth. This coating method is suitable to apply biofertilizer for direct sowing cultivation of rice.

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Spore inoculation of Bacillus pumilus TUAT1 strain, a biofertilizer microorganism, enhances seedling growth by promoting root system development in rice

Cited by 12 publications

References 29 publications

Potential of Bacillus pumilus to directly promote plant growth

Potential of Bacillus pumilus to directly promote plant growth

Plant Growth-promoting Effects of Viable and Dead Spores of <i>Bacillus pumilus</i> TUAT1 on <i>Setaria viridis</i>

Seed coating by biofertilizer containing spores of Bacillus pumilus TUAT1 strain enhanced initial growth of Oryza sativa L.

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