Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting

Gerdemann, J. W.; Nicolson, T. H.

doi:10.1016/s0007-1536(63)80079-0

Cited by 2,954 publications

(1,355 citation statements)

References 18 publications

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“…The spore count of the inoculum was 49 spores/g soil as determined by the wet sieving and decanting method of Gerdemann and Nicolson (1963). Inoculum (15 g) was mixed with the soil of each pot for the mycorrhizal treatment, and 15 g sterilized inoculum plus 15-mL mycorrhizal fungal-free filtrate from the inoculum suspension were added for the non-mycorrhizal treatment to provide a similar microflora except for the mycorrhizal fungus.…”

Section: Experimental Conditionsmentioning

confidence: 99%

Yield and arsenate uptake of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167 in As spiked soil under glasshouse conditions

Liu

Zhu

Chen

et al. 2005

Environment International

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A glasshouse pot experiment was conducted to study the effect of arbuscular mycorrhizal (AM) colonization by Glomus mosseae BEG167 on the yield and arsenate uptake of tomato plants in soil experimentally contaminated with five As levels (0, 25, 50, 75 and 150 mg kg À 1 ). Mycorrhizal colonization (50 -70% of root length) was little affected by As application and declined only in soil amended with 150 mg As kg À 1 . Mycorrhizal colonization increased plant biomass at As application rates of 25, 50 and 75 mg kg À 1 . Shoot As concentration increased with increasing As addition up to 50 mg kg À 1 but decreased with mycorrhizal colonization at As addition rates of 75 and 150 mg kg À 1 . Shoot As uptake increased with mycorrhizal colonization at most As addition levels studied, but tended to decrease with addition of 150 mg As kg À 1 . Total P uptake by mycorrhizal plants was elevated at As rates of 25, 50 and 75 mg kg À 1 , and more P was allocated to the roots of mycorrhizal plants. Mycorrhizal plants had higher shoot and root P/As ratios at higher As application rates than did non-mycorrhizal controls. The soil of inoculated treatments had higher available As than uninoculated controls, and higher pH values at As addition levels of 25, 50 and 75 mg kg À 1 . Mycorrhizal colonization may have increased plant resistance to potential As toxicity at the highest level of As contamination studied. Mycorrhizal tomato plants may have potential for phytoextraction of As from moderately contaminated soils or phytostabilization of more highly polluted sites. D

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Section: Experimental Conditionsmentioning

confidence: 99%

Yield and arsenate uptake of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167 in As spiked soil under glasshouse conditions

Liu

Zhu

Chen

et al. 2005

Environment International

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“…Isolation of dominant AM fungi from soil samples: Isolation of dominant AM spores were done by using 'Wet Sieving and Decanting Technique' of Gerdemann and Nicolson (1963). Spores were then picked by hypodermic needle under stereobinocular microscope.…”

Section: Methodsmentioning

confidence: 99%

Impact of endomycorrhizal fungi and other bioinoculants on growth enhancement of Glycine max (L.) Merrill

Alpa

Neetu

Tanwar

et al. 2012

JANS

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Abstract:In the present investigation, the contributions of two indigenous arbuscular mycorrhizal fungi (Glomus mosseae and Acaulospora laevis), along with Trichoderma viride and Bradyrhizobium japonicum on growth parameters of Soybean, Glycine max (L.) Merrill were investigated. The results obtained indicated the dependence of soybean on mycorrhizal symbiosis. The different growth parameters increased significantly after 120 days of inoculation in comparison to control. Among all the growth parameters studied, plant height (162±3.34), fresh shoot weight (31.26±1.45), dry shoot weight (3.52±0.05), fresh root weight (4.07±0.56), dry root weight (1.03±0.03), root length (49.0±4.47) and leaf area (32.58±1.70) were highest in the combination of G. mosseae + A. laevis + T. viride + B. japonicum but arbuscular mycorrhizal (AM) spore number (95.2±3.19) and percent mycorrhizal root colonization (93.26±3.96) were maximum in single inoculation of G. mosseae. Second most effective results were observed in the plants treated with G. mosseae alone. Thus the presence of arbuscular mycorrhizal fungi (AMF) and other bioinoculants in rhizosphere of soybean had positive effect on the different growth parameters.

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“…The spores were classified in agreement with Morton [23] and Shenck and Perez [24] and the multiplication was done on Marglobe variety tomato seedlings grown in sand that had been washed, sterilized and supplemented with a phosphorus deficient Hoagland and Arnon solution [25]. After 60 days, spores were separated by wet sieving and the suspensions were prepared according to Genderman and Nicolson [26]. The suspension of 100 spores per milliliter of sterile distilled water was used as the inoculum.…”

Section: The Arbuscular Micorrhizal Colonizationmentioning

confidence: 99%

Interaction of Azospirillum brasilense and Glomus intrarradix in Sugar Cane Roots

Bellone

2011

Indian J Microbiol

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Fifteen-day-old variety NA 56-79 sugar cane seedlings were inoculated with Azospirillum brasilense and Glomus intrarradix. This article aims at examining changes in sugar cane root seedlings inoculated with Glomus intrarradix and Azospirillum brasilense, the increase in microbial biomass and the acetylene reduction process as well. The internal root colonization was studied 20 days after inoculation using scanning and a transmission electron microscope. Both microorganisms entered the sugar cane root through the emergent lateral roots. The microorganisms were capable of coexisting both intra and intercellularly, producing changes in the cell wall, thus allowing colonization and interaction between the organisms. These changes increased the number of microorganisms inside the root as well as acetylene nitrogen reduction. Sugar cane plant biomass increased with jointinoculation. The number of endophytic microorganisms and nitrogen fixing activity increased when they were colonized by Azospirillum and Glomus together.

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Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting

Cited by 2,954 publications

References 18 publications

Yield and arsenate uptake of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167 in As spiked soil under glasshouse conditions

Yield and arsenate uptake of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167 in As spiked soil under glasshouse conditions

Impact of endomycorrhizal fungi and other bioinoculants on growth enhancement of Glycine max (L.) Merrill

Interaction of Azospirillum brasilense and Glomus intrarradix in Sugar Cane Roots

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