Spreading of Epithelial Cells on Machined and Sandblasted Titanium Surfaces: An In Vitro Study

Carmine, Maristella Di; Toto, Paola; Feliciani, Claudio; Tulli, Antonello; Strocchi, R.; Piattelli, Adriano

doi:10.1902/jop.2003.74.3.289

Cited by 25 publications

(23 citation statements)

References 31 publications

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“…The filopodia were extended and evenly distributed over the surface. This phenomenon of altered epithelial morphology is indicating that XB-2 cells have a better growth rate on the rough surface [19]. …”

Section: Resultsmentioning

confidence: 99%

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A Comparison of Epithelial Cells, Fibroblasts, and Osteoblasts in Dental Implant Titanium Topographies

Teng

Kuo

et al. 2012

Bioinorganic Chemistry and Applications

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The major challenge for dental implants is achieving optimal esthetic appearance and a concept to fulfill this criterion is evaluated. The key to an esthetically pleasing appearance lies in the properly manage the soft tissue profile around dental implants. A novel implant restoration technique on the surface was proposed as a way to augment both soft- and hard-tissue profiles at potential implant sites. Different levels of roughness can be attained by sandblasting and acid etching, and a tetracalcium phosphate was used to supply the ions. In particular, the early stage attaching and repopulating abilities of bone cell osteoblasts (MC3T3-E1), fibroblasts (NIH 3T3), and epithelial cells (XB-2) were evaluated. The results showed that XB-2 cell adhesive qualities of a smooth surface were better than those of the roughened surfaces, the proliferative properties were reversed. The effects of roughness on the characteristics of 3T3 cells were opposite to the result for XB-2 cells. E1 proliferative ability did not differ with any statistical significance. These results suggest that a rougher surface which provided calcium and phosphate ions have the ability to enhance the proliferation of osteoblast and the inhibition of fibroblast growth that enhance implant success ratios.

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Section: Resultsmentioning

confidence: 99%

“…Early stage cell abilities such as adhesion and proliferation to the substrates can vary according to surface topography, which in turn influences cytoskeletal components [19, 23]. Sandblasting was thought to induce stress on the surface, whereas acid etching was thought to release the resulting residual stresses.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Epithelial Cells, Fibroblasts, and Osteoblasts in Dental Implant Titanium Topographies

Teng

Kuo

et al. 2012

Bioinorganic Chemistry and Applications

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“…Once the phase of osteoclastic resorption is over and osteoclasts disappear from the resorption site, osteoblasts and their precursors repopulate the resorption site and reline the bone surface 8. Implant surface roughness has been demonstrated to affect the characteristics and the behavior of the osteoblasts at the bone–implant interface, and a higher bone–implant contact percentage has been reported for implants with a rougher surface 9–15. Although osteoblasts play a pivotal role in implant integration in the bone context, osteoclast activity is also required for peri‐implant bone remodeling 16.…”

Section: Introductionmentioning

confidence: 99%

Implant surface roughness influences osteoclast proliferation and differentiation

et al. 2005

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The osteoclast is a hematopoietic cell derived from CFU-GM and branches from the monocyte-macrophage lineage during the differentiation process. Biological environment appears to be crucial for osteoclast formation and activity. It has been reported that bone remodeling following implant placement requires a coordinated activity by osteoclasts and osteoblasts. The response of such cells at the bone-implant interface has been suggested to be affected by the structural and morphological features of the biomaterial surface. To shed more light on this topic we performed a multiparametric analysis of murine monocytes response to different titanium surfaces. These cells, RAW 264.7 type TIB-71, represent a very useful system because they differentiate into osteoclasts following treatment of definite doses of the osteoclast-differentiation factor RANKL and macrophage colony-stimulating factor (M-CSF). Cells, cultured on glass (control), on grade 3 machined and on titanium pull-spray superficial-TPSS surfaces disclosed profound different responses in terms of morphological rearrangements, adhesion, and differentiation abilities. Indeed, after 14 days, cells cultured on glass and machined surfaces were uniformly distributed, while, on the TPSS surface cells strictly aggregated into small isolated clusters were observed. In addition, cells cultured on the machined surface displayed a higher adhesion ability, while cells cultured on the rougher surface disclosed a more evident capability to differentiate. These results could explain the higher bone-implant contact percentage found around implants with rougher surfaces and suggest that osteoclasts may play an important role in the initial period after implant placement to prime or prepare the implant surface for the osteoblast activity.

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“…Porous implants can provide a safer barrier against infection if invaded by the host tissues at early stages after implantation [7]. The better epithelial cell adhesion and spreading decreases a risk of detachment (avulsion) of the surrounding tissues from the implant [8, 9]. These facts make porous structures promising for implantation to the residuum bone with the purpose of direct skeletal attachment (DSA) of limb prostheses [10, 11].…”

Section: Introductionmentioning

confidence: 99%

Mechanical properties of totally permeable titanium composite pylon for direct skeletal attachment

Pitkin¹,

Pilling²,

Raykhtsaum³

2012

J Biomed Mater Res

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Composite pylons containing a solid titanium core with drilled holes surrounded by a porous sintered titanium shell have been fabricated and tested in bending along with the raw cores and pylons composed of the porous titanium alone. The new pylons were designed with the concept of enhanced ingrowth of bone and skin cells and are intended for direct skeletal attachment of limb prostheses considering requirements for long-lasting anchorage to the residuum bone and a need for a safe skin-implant seal. Load-displacement thresholds were determined after which the integrity of the porous component may be compromised. The composite pylons have a flexural strength and stiffness substantially greater than that of pylons composed of the porous titanium alone. The drilled holes in the solid insert have been shown to have virtually no effect on the flexural strength of the pylon, while meeting a requirement for total permeability of the device for unrestricted cell ingrowth. The predicted strength of the pylons and associated failure modes are in close agreement with those measured.

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Spreading of Epithelial Cells on Machined and Sandblasted Titanium Surfaces: An In Vitro Study

Abstract: These results indicate that sandblasted surfaces are the optimal substrata for epithelial cell adhesion and spreading.

Cited by 25 publications

References 31 publications

A Comparison of Epithelial Cells, Fibroblasts, and Osteoblasts in Dental Implant Titanium Topographies

A Comparison of Epithelial Cells, Fibroblasts, and Osteoblasts in Dental Implant Titanium Topographies

Implant surface roughness influences osteoclast proliferation and differentiation

Mechanical properties of totally permeable titanium composite pylon for direct skeletal attachment

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