SRAP Molecular Markers Linked to Three Morphological Traits in Egyptian Bread Wheat (Triticum aestivum L.)

Abstract

Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the genetic diversity of eight Egyptian bread wheat (Triticum aestivum L.) genotypes. Five SRAP primers combinations were able to expose the polymorphism between the studied genotypes. The percentage of polymorphism (P %) ranged from 40 to 79.90 %. Furthermore, the Polymorphic Information Content (PIC) values for SRAP primers combination varied from 0.11 to 0.31 with an average of 0.19. The Me5F-Em2R and Me1F-Em2R primer combination cleared higher levels of polymorphism of 66.67 and 76.90%, respectively. The results of Single Marker Analysis (SMA) showed that the SRAP marker Me1F-Em2R 1320bp could be considered probably as candidate marker linked to spike length in the tested genotypes. Moreover, Me1F-Em2R 900bp and Me1F-Em2R 575bp could be considered as markers linked to plant height and tillers number /plant traits, respectively. Results of Single Marker Analysis (SMA) showed significant marker-trait associations for spike length (P= 0.04), plant height (P= 0.02) and number of tillers/plant (P= 0.03). Me1F-Em6R 850bp marker could be also considered as candidate markers probably linked to plant height, with a specific fragment of 850bp for the tolerant genotype (P1). UPGMA cluster analysis based on SRAP markers separated the studied wheat genotypes into three significantly different clusters. The dendrogram based on morphological traits divided the studied wheat genotypes into two different clusters. The Mantel test revealed that there was a positive but non-significant correlation between the genetic similarities based on phenotypic data and SRAP marker (r = 0.36, P> 0.05).

Correlation between Genetic- physiological Traits of Egyptian Lupin (Lupinus termis) Induced by Drought Stress

“…On the other hand, Khaled et al (2019a) found 39 bands from 82 were polymorphic when using molecular markers (SRAP) for seven Sorghum bicolor genotypes. Also, Khaled et al (2019b) obtained 64 polymorphic bands from a total of 95, with a total number of bands ranging from two to twelve. Their polymorphic bands ranged from 200 bp to 1750 bp, in the same range as the present study, whereas the size of the obtained fragment ranged from 150 to 1700 bp.…”

“…SRAP markers are frequently employed in plant genetic diversity identification and genetic map development ( Khaled et al, 2019a;El-Sherbeny et al, 2020;Qiao et al, 2022). Genetic diversity using SRAP markers was used among lupin genotypes (El-harty et al, 2016) and many other crop plants like soybean genotypes (Sun et al, 2013;El-Sherbeny et al, 2020), Wheat (Khaled et al, 2019b), Sorghum (Khaled et al, 2019a), and Cucurbita plant (Ferriol et al, 2003;Inan et al, 2012). This study attempts to look into how the Lupinus termis plant responds physiologically and molecularly to drought stress using SRAP marker, growth criteria, and some physiological parameters.…”

Evaluating the genetic diversity in maize hybrids under drought conditions using drought indices, SSR markers, and thermal imaging

“…Some drought indices were used to evaluate and identify genotypes under normal and drought conditions; Selection index was more effective than direct selection for grain yield of some crops (Grezsiak et al, 2012;Bayoumi et al, 2015;Gabr Afaf et al, 2018;Emam et al, 2022). On the other hand, the using of molecular markers such as simple sequence repeat (SSR) markers present a promising method to identify stress tolerance genes due to the fact that it characterized by its recognition of codominancy (Bawa et al, 2015;Elci and Hancer, 2015;Younis et al, 2020;Mdluli et al, 2020;Khaled et al, 2021).SSR markers were used for doing molecular characterization for twenty-four lines of maize was applied in previous studies (Gazal et al, 2016;Kamara et al, 2020) Also; hundred maize lines using a set of 32 SSR markers having genome wide coverage, which supports the significantly contributed of SSR markers to identify drought tolerant genotypes (Gazal et al, 2018). Several crops had been characterized according to their response to environmental stresses by using thermal imaging (Munns et al, 2010;Walter et al, 2012).…”