Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolones and corticosteroids in ophthalmic formulations

Abstract

We develop and validate a stability-indicating assay method for simultaneous determination of gatifloxacin and prednisolone acetate, or of ciprofloxacin hydrochloride and dexamethasone in combination and in the presence of degradation products.

Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolone in combination with a non-steroidal anti-inflammatory drug in pharmaceutical formulation

“…The factors (chromatographic conditions) selected to examine the robustness were the flow rate, percentage of acetonitrile, and pH of the water in the mobile phase. Each factor was investigated at three levels (-1, 0, and + 1) (Sversut et al, 2014). Level 0 refers to the normal chromatographic conditions, i.e., the conditions employed in the proposed method.…”

Stepwise optimization and sensitivity improvement of green micellar electrokinetic chromatography method to simultaneously determine some fluoroquinolones and glucocorticoids present in various binary ophthalmic formulations

“…Various methods have been reported describing the assay of the fluoroquinolones and corticosteroids either singly or in combination. These methods include spectrophotometry (Abdel‐Razeq, Darwish, Zaazaa, Nasr, & Zeinab, 2015; Patel & Patel, 2013; Pradhan, Raiyani, Shah, Patel, & Upadhyay, 2015; Sversut et al, 2017), HPLC (El Gammal, El‐Wasseef, El‐Ashry, & Saadia, 2018; Gandhi, Rao, & Rao, 2016; Saad, Essam, Elzanfaly, & Amer, 2020; Sher, Fatima, Perveen, & Siddiqui, 2019; Sversut et al, 2014), thin‐layer chromatography (TLC) (Dorofeev, Konovalov, Kochin, & Arzamastsev, 2004; Musharraf, Fatima, & Sultana, 2012; Saad et al, 2020; Seid, Hymete, & Bekhit, 2012), capillary electrophoresis (Al Azzam, Saad, Adnan, & Aboul‐Enein, 2010; Alnajjar, 2013; Bourdon et al, 2013; Gallego & Arroyo, 2003; Horstkötter & Blaschke, 2001; Maher et al, 2013; Olędzka, Kowalski, Plenis, & Bączek, 2017; British Pharmacopoeia, 2013; Song, Bai, Jia, & Zhou, 2008; Suliman, Elbashir, & Schmitz, 2015; Wang et al, 2009; Zhu, Jiang, & Hu, 2002) and electrochemical methods (El‐Rahman, Lotfy, Hegazy, Rezk, & Rostom, 2015; Smajdor, Piech, & Paczosa‐Bator, 2016; Zhang, Gu, Ding, Li, & Liu, 2013; Zhang, Gu, Ding, Zhang, & Li, 2013). After a thorough investigation of literature, it was observed that no capillary electrophoretic method has been published for the simultaneous assay of the four chosen drugs.…”

Chromatographic-Densitometric Analysis of Chosen Fluoroquinolones on TLC Plates Using Mobile Phases with Different Viscosity

“…Similar methods have been used for determination of some group of fluoroquinolones in tilapia samples [5] , in eggs [6] , in milk [7] and in sheep plasma [8] as veterinary drug residues. The HPLC was used also for investigation of some fluoroquinolones in pharmaceutical preparations [9][10][11][12] . These methods have been reported for determination of the fluoroquinolones individually [9] or in different combinations [10][11][12] .…”

“…The HPLC was used also for investigation of some fluoroquinolones in pharmaceutical preparations [9][10][11][12] . These methods have been reported for determination of the fluoroquinolones individually [9] or in different combinations [10][11][12] .…”