Stability-Indicating UPLC Method for the Estimation of Nadifloxacin, Terbinafine Hydrochloride, Mometasone Furoate, Methyl Paraben, and Propyl Paraben in Topical Pharmaceutical Dosage Form

Abstract

A novel and simple ultra-performance LC method was developed for the estimation of nadifloxacin (NAD), terbinafine hydrochloride (TBH), mometasone furoate (MMF), methyl paraben (MP), and propyl paraben (PP) in a topical pharmaceutical dosage formulation. The analysis was carried out on a Waters Acquity UPLC ethylene bridged hybrid C18 column (50 × 2.1 mm, 1.7 μm) with a flow rate of 0.4 mL/min in gradient mode at a wavelength of 255 nm. Elution of all components was achieved within 9 min. The retention times of MP, NAD, PP, TBH, and MMF were observed at 1.5, 2.6, 3.4, 6.0, and 6.9 min, respectively. The proposed method was validated per current International Conference on Harmonization guidelines for specificity, precision, linearity, accuracy, range, LOD, LOQ, robustness, filter paper interference, and solution stability parameters. A complete method study was performed to determine the stability-indicating nature of the developed method.

Analysis of terbinafine in PLGA-based drug delivery systems by a fast and sensitive UHPLC-DAD method

“…Often, the separation of only terbinafine or its blend with other drugs that never occur together in one pharmaceutical preparation has been reported. Bhosale et al 8 separated nadifloxacin, terbinafine hydrochloride, and mometasone furoate occurring in a single preparation. However, they have never tried using their method to analyze real samples.…”

Excessive amounts of antifungal agent and preservatives were illegally added in an adulterated herbal product for kids

“…In order to determine the content of these illegally added ingredients in the creams, it is essential to establish a simple, sensitive and accurate analytical method. A literature survey revealed that several analytical methods have been published for the assay of Terbinafine hydrochloride, Methylparaben and Propylparaben (Priyanka & Sk, 2014;Dewani et al, 2015;Bhosale, & Nikalje, 2017). These methods utilized buffer solutions to stabilize the analytes.…”

Dispersive liquid-liquid microextraction of parabens from pharmaceuticals andpersonal care products prior to their determination using HPLC-DAD

“…Analysis of pharmaceuticals and PCPs for parabens has been performed using flow-injection ultraviolet spectrophotometry (FI-UV) [6], gas chromatography-mass spectrometry (GC-MS) [7] and flame-ionization detection (GC-FID) [8], capillary electrophoresis-UV (CE-UV) [9], and ultra-performance liquid chromatography (UPLC) [10]. High-performance liquid chromatography-ultraviolet (HPLC-UV) remains the most common analytical technique for this purpose.…”