Stability of antibacterial self-assembled monolayers on hydroxyapatite

Torres, Nelson S.; Oh, Se Baek; Appleford, Mark R.; Dean, David D.; Jorgensen, James H.; Ong, Joo L.; Agrawal, C. Mauli; Mani, Gopinath

doi:10.1016/j.actbio.2010.02.032

Cited by 37 publications

(30 citation statements)

References 40 publications

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“…The presence of 11-PUDA and/or 16-PHDA SAMs did not significantly affect the contact angle, and this observation was in agreement with contact angle values reported in the literature [36]. However, the attachment of mixed SAMs with a ratio of 50:50 (11-PUDA:16-PHDA) resulted in a significantly different contact angle compared to the control and homogeneous SAM groups.…”

Section: Discussionsupporting

confidence: 93%

“…The most extensively studied SAMs are silanes on gold, but many other systems have been evaluated. For example, octadecylphosphonic acid has been shown to form SAMs on mica surfaces [35], while other phosphonic acids including 11-phosphonoundecanoic acid (11-PUDA) and 16-phosphonohexadecanoic acid (16-PHDA) have been reported to be stable on HA surfaces [36]. The mechanism of attachment for phosphonic acid SAMs on HA surfaces is the formation of surface phosphonates (C-PO(OR) 2 ) between phosphonic acid molecules and the P-OH groups in HA.…”

Section: Introductionmentioning

confidence: 99%

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Vascular endothelial growth factor attachment to hydroxyapatite via self-assembled monolayers promotes angiogenic activity of endothelial cells

Solomon

Ong

2013

Thin Solid Films

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Vascular endothelial growth factor attachment to hydroxyapatite via self-assembled monolayers promotes angiogenic activity of endothelial cells

Solomon

Ong

2013

Thin Solid Films

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“…A modified protocol of standard bacterial adhesion tests was used to quantify the viable colony forming units of bacteria per surface area of the films (CFU/cm 2 ) [66–68]. A single colony of bacteria was isolated from a previously cultured LB-agar plate and incubated in LB medium for 14 h at 37°C at a rotating speed of 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

A multi-defense strategy: Enhancing bactericidal activity of a medical grade polymer with a nitric oxide donor and surface-immobilized quaternary ammonium compound

et al. 2017

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Although the use of biomedical devices in hospital-based care is inevitable, unfortunately, it is also one of the leading causes of the nosocomial infections, and thus demands development of novel antimicrobial materials for medical device fabrication. In the current study, a multi-defense mechanism against Gram-positive and Gram-negative bacteria is demonstrated by combining a NO releasing agent with a quaternary ammonium antimicrobial that can be covalently grafted to medical devices. Antibacterial polymeric com posites were fabricated by incorporating a nitric oxide (NO) donor, S-nitroso-N-acetyl-penicillamine (SNAP) in CarboSil® polymer and top coated with surface immobilized benzophenone based quaternary ammonium antimicrobial (BPAM) small molecule. The results suggest that SNAP and BPAM have a different degree of toxicity towards Gram-positive and Gram-negative bacteria, and the SNAP-BPAM combination is effective in reducing both types of adhered viable bacteria equally well. SNAP-BPAM combinations reduced the adhered viable Pseudomonas aeruginosa by 99.0% and Staphylococcus aureus by 99.98% as compared to the control CarboSil films. Agar diffusion tests demonstrate that the diffusive nature of NO kills bacteria beyond the direct point of contact which the non-leaching BPAM cannot achieve alone. This is important for potential application in biofilm eradication. The live-dead bacteria staining shows that the SNAP-BPAM combination has more attached dead bacteria (than live) as compared to the controls. The SNAP-BPAM films have increased hydrophilicity and higher NO flux as compared to the SNAP films useful for preventing blood protein and bacterial adhesion. Overall the combination of SNAP and BPAM imparts different attributes to the polymeric composite that can be used in the fabrication of antimicrobial surfaces for various medical device applications.

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“…The ability of bisphosphonates to lock onto the mineral (HA) fraction of bone via their polar, phosphor head groups is widely recognised [26]. This ability to avidly bind HA was exploited by Torres and coworkers [27] who used an inert alkane PA, octadecylphosphonic acid (ODPA), for the subsequent addition of silver to create an antibacterial HA finish.…”

Section: Introductionmentioning

confidence: 99%

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Neary

Blom

Shiel

et al. 2018

J Mater Sci: Mater Med

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There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents to encourage superior integration of the implants into host bone. One such agent is (3S) 1-fluoro-3-hydroxy-4-(oleoyloxy) butyl-1-phosphonate (FHBP), a phosphatase-resistant lysophosphatidic acid (LPA) analogue. We investigated the effect of an FHBP-HA coating on the maturation of human (MG63) osteoblast-like cells. Optimal coating conditions were identified and cell maturation on modified and unmodified, control HA surfaces was assessed. Stress tests were performed to evaluate coating survivorship after exposure to mechanical and thermal insults that are routinely encountered in the clinical environment. MG63 maturation was found to be three times greater on FHBP-modified HA compared to controls (p < 0.0001). There was no significant loss of coating bioactivity after autoclaving (P = 0.9813) although functionality declined by 67% after mechanical cleaning and reuse (p < 0.0001). The bioactivity of modified disks was significantly greater than that of controls following storage for up to six months (p < 0.001). Herein we demonstrate that HA can be functionalised with FHBP in a facile, scalable manner and that this novel surface has the capacity to enhance osteoblast maturation. Improving the biological performance of HA in a bone regenerative setting could be realised through the simple conjugation of bioactive LPA species in the future. Depicted is a stylised summary of hydroxyapatite (HA) surface modification using an analogue of lysophosphatidic acid, FHBP. a HA surfaces are simply steeped in an aqueous solution of 2 μM FHBP. b The polar head group of some FHBP molecules react with available hydroxyl residues at the mineral surfaces forming robust HA-O-P bonds leaving acyl chain extensions perpendicular to the HA surface. These fatty acyl chains provide points of integration for other FHBP molecules to facilitate their self-assembly. This final surface finish enhanced the human osteoblast maturation response to calcitriol, the active vitamin D3 metabolite.

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Stability of antibacterial self-assembled monolayers on hydroxyapatite

Cited by 37 publications

References 40 publications

Vascular endothelial growth factor attachment to hydroxyapatite via self-assembled monolayers promotes angiogenic activity of endothelial cells

Vascular endothelial growth factor attachment to hydroxyapatite via self-assembled monolayers promotes angiogenic activity of endothelial cells

A multi-defense strategy: Enhancing bactericidal activity of a medical grade polymer with a nitric oxide donor and surface-immobilized quaternary ammonium compound

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

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