Stability of small mesoporous silicananoparticles in biological media

Yu, Lin; Abadeer, Nardine S.; Haynes, Christy L.

doi:10.1039/c0cc02923h

Cited by 166 publications

(174 citation statements)

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“…The biocompatibility and biodegradability of PEG has been demonstrated in a number of studies [30,31]. Moreover, the short-and long-term influence of PEGylation on biodegradability of MSNs in different media including aqueous solutions [32], simulated body fluids (SBF) [33], phosphate buffered saline (PBS) [34], and Dulbecco's modified eagle's medium (DMEM) with fetal bovine serum (FBS) [32] has been evaluated. Although the biodegradability of these nanoparticles in complex environments is relatively lower than that in aqueous solutions, however, PEGylation shows an overall enhancing effect on MSN biostability, yielding to a decreased loss of mesoporous parameters such as surface area and pore volume [32,33].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the short-and long-term influence of PEGylation on biodegradability of MSNs in different media including aqueous solutions [32], simulated body fluids (SBF) [33], phosphate buffered saline (PBS) [34], and Dulbecco's modified eagle's medium (DMEM) with fetal bovine serum (FBS) [32] has been evaluated. Although the biodegradability of these nanoparticles in complex environments is relatively lower than that in aqueous solutions, however, PEGylation shows an overall enhancing effect on MSN biostability, yielding to a decreased loss of mesoporous parameters such as surface area and pore volume [32,33]. The PEG coating could also improve the colloidal stability of MSNs by increasing their hydrophilicity as well as the steric distances, which in turn reduces the attraction forces between the nanoparticles [14].…”

Section: Discussionmentioning

confidence: 99%

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Low-melting-point polymeric nanoshells for thermal-triggered drug release under hyperthermia condition

Dabbagh

Mahmoodian

Abdullah

et al. 2015

International Journal of Hyperthermia

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Purpose: The aim of this paper was to synthesise core-shell nanostructures comprised of mesoporous silica core and a low melting-point polyethylene glycol (PEG) nanoshell with a sharp gel-liquid phase transition for rapid drug release at hyperthermia temperature range. Materials and methods:The phase transition behaviours of PEGs with molecular weights of 1000, 1500, and 2000 Da were analysed using differential scanning calorimetry (DSC) to determine the optimal formulation with phase transition in the hyperthermia range. The 'graftto' method was employed to synthesise core-shell nanostructures using the selected PEG formulation. The drug loading and release behaviours of these nanocarriers were examined by ultra-violet visible spectroscopy (UV-Vis) using doxorubicin as a model drug. Magnetic resonance-guided focused ultrasound (MRgFUS) was also applied as a typical thermal modality to evaluate the rate of drug release from the core-shell nanostructures. Results: The PEG molecular weight of 1500 Da presented the optimal phase transition temperature for thermaltriggered release under hyperthermia conditions. Drug release measurements at different temperatures using UV-Vis methods showed a 20.2 ± 4.3% leakage in aqueous solution at 37 C after 30 min, while this value was significantly increased to 68.2 ± 3.7% at 50 C. A 45.5 ± 3.1% drug release was also obtained after sonication of the drug-loaded nanoparticles for 5 Â 20 s using MRgFUS. Conclusion: Although the ratio of drug leakage at physiological temperatures was relatively high, the sharp transition temperature, high loading efficiency, and fast drug release at hyperthermia temperature range could make these core-shell nanoparticles prominent for enhancing the efficacy of various hyperthermia modalities in the treatment of cancer tumours.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Low-melting-point polymeric nanoshells for thermal-triggered drug release under hyperthermia condition

Dabbagh

Mahmoodian

Abdullah

et al. 2015

International Journal of Hyperthermia

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“…13 Further, it was observed that: (i) smaller Stöber silica nanoparticles (24 nm) induce a pronounced hemolytic effect when compared with bigger ones (263 nm); 14 (ii) nanostructures with high aspect ratio (nanorods) are more cytotoxic than spherical nanoparticles; 15 (iii) mesoporous silica nanostructures (MSNs) with ordered pores (MCM-41) induce a stronger hemolytic effect compared with non-ordered; 14 (iv) small mesoporous nanoparticles (20 nm) consisted of ethenylenebridged silsesquioxane present very low toxicity 16 and (v) polymers such as PEG (polyethylene glycol) can be used to coat particles in order to greatly reduce the hemolysis. 17 Furthermore, an extensive assessment of the interaction of bare and functionalized porous silica nanomaterials with RBCs concluded that SBA-15-type MSNs cause the deformation of RBCs and consequently lead to their disruption. Amine functionalizations on the surfaces of MSNs also reduce the hemolytic effect.…”

Section: Introductionmentioning

confidence: 99%

Suppression of the hemolytic effect of mesoporous silica nanoparticles after protein corona interaction: independence of the surface microchemical environment

Paula¹,

Martinez²,

Júnior³

et al. 2012

J. Braz. Chem. Soc.

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Nanopartículas mesoporosas de sílica são conhecidas por induzirem hemólise de células vermelhas do sangue (RBCs) humano quando ensaios de citotoxicidade são feitos em solução-tampão de fosfato (PBS). Entretanto, em uma abordagem mais realista, a presença de biomoléculas do plasma sanguíneo precisa ser considerada em qualquer avaliação nanotoxicológica de nanopartículas porosas de SiO 2 quando se objetiva a sua utilização em aplicações biomédicas através de administração intravenosa. Nesse contexto, demonstrou-se neste trabalho que nanopartículas porosas de sílica não induzem nenhum efeito citotóxico em células vermelhas do sangue quando ensaios de hemólise são feitos na presença de plasma sanguíneo, independentemente da carga superficial (positiva ou negativa) da nanopartícula. A ausência de hemólise está principalmente associada à adsorção de proteínas do plasma sobre a superfície das nanopartículas, levando à formação de um recobrimento proteico estável (denominado protein corona ou PC) que blinda o ambiente microquímico original das nanopartículas.Mesoporous silica nanoparticles are known to induce the hemolysis of human red blood cells (RBCs) when citotoxicity assays are performed in a phosphate buffer solution (PBS). However, in a more realistic approach, the presence of blood plasma biomolecules must be considered in any nanotoxicological evaluation of porous SiO 2 nanoparticles when biomedical applications through intravenous administration are aimed. In this context, it is demonstrated in this work that porous silica nanoparticles do not induce any cytotoxic effect on RBCs when hemolysis assay is done in the presence of blood plasma, regardless the surface charge (positive or negative) of the nanoparticle. The absence of hemolysis is mainly associated with the adsorption of plasma proteins on the nanoparticle surface, which leads to the formation of a stable protein coating (called protein corona or PC) that shields the original microchemical environment of bare nanoparticles.Keywords: nanoparticles, SiO 2 , mesopores, hemolytic effect, protein corona IntroductionSince porous silica nanoparticles were elected as possible protagonists in a future revolution of several medical processes of theranosis, they have been widely studied during the last decade through the host-guest approach, thus resulting in promising perspectives mainly in the areas of detection 1-3 and treatment of tumors. [4][5][6][7] While part of the scientific community creatively advances towards the engineering of porous silica nanostructures, others are acting in a proactive approach by considering environmental and toxicological effects of nano-based silica materials. In the latter context, several in vitro citotoxicity assays indicated a very high biocompatibility of porous silica nanoparticles. [8][9][10] However, a desirable in vivo biocompatibility is not straightforward. For instance, it is well known that amorphous silica particles induce toxicity on human red blood cells (RBCs) and, consequently, this test is being used as a ke...

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“…In the work published by Lin et al, the influence of a hydrophilic polymer shell (poly(ethylene glycol), PEG) was investigated by incubating the MSNPs in PBS and performing a silicomolybdic blue assay. It was found that nonpegylated MSNPs had greater degradation than pegylated MSNPs [12]. Moreover, Cauda et al reported that longer and denser PEG shells resulted in slower biodegradation kinetics [13].…”

Section: Introductionmentioning

confidence: 99%

Altering the Biodegradation of Mesoporous Silica Nanoparticles by Means of Experimental Parameters and Surface Functionalization

Seré

Roo

Vervaele

et al. 2018

Journal of Nanomaterials

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Mesoporous silica nanoparticles (MSNPs) are gaining a large interest in the field of medical and biomedical applications due to their biodegradability and high loading capacity as a carrier. In this work, a simple synthesis and functionalization procedure is reported, which allows tuning the nanoparticle properties, functionalization, and biodegradability. Variations in the synthesis procedure are introduced, including temperature, concentration of catalyst, and surface functionalization. These samples are characterized and afterwards degraded in phosphate buffered saline (PBS) to determine their degradation kinetics. The amount of degraded material is colorimetrically determined, using an optimized protocol based on molybdenum blue chemistry. It is shown that the degradability of the nanoparticles increased with decreasing synthesis temperatures, lower amounts of catalyst, and higher concentrations of nanoparticles. Surface functionalization alters the degradation kinetics as well, rendering amino-functionalized nanoparticles the fastest degradation behavior, followed by carboxylated and nonfunctionalized nanoparticles. From these results, it can be concluded that the degradation rate of MSNPs can be varied from a few hours to several days by small changes in the synthesis procedure. Moreover, the degradation behavior is strongly dependent on the nanoparticle growth rate.

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Stability of small mesoporous silicananoparticles in biological media

Cited by 166 publications

References 20 publications

Low-melting-point polymeric nanoshells for thermal-triggered drug release under hyperthermia condition

Low-melting-point polymeric nanoshells for thermal-triggered drug release under hyperthermia condition

Suppression of the hemolytic effect of mesoporous silica nanoparticles after protein corona interaction: independence of the surface microchemical environment

Altering the Biodegradation of Mesoporous Silica Nanoparticles by Means of Experimental Parameters and Surface Functionalization

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