Stabilization of lysosomal membrane and cell membrane glycoprotein profile bySemecarpus anacardium Linn. nut milk extract in experimental hepatocellular carcinoma

Balachandran, Premalatha; Sachdanandam, Panchanatham

doi:10.1002/1099-1573(200008)14:5<352::aid-ptr645>3.0.co;2-c

Cited by 9 publications

(1 citation statement)

References 23 publications

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“…The drug SA contains a number of minerals and vitamins. Analysis of SA revealed the presence of calcium, iron, copper, sodium and aluminium 44) and it's also proved the antiproliferative effects on several cancer cells. 22) Other constituents of the drug namely bhilawanols are mainly localized maximally in cell membrane fraction suggesting their effect through alterations in membrane fractions.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Effect of Kalpaamruthaa on Mammary Carcinoma in Rats with Reference to Glycoprotein Components, Lysosomal and Marker Enzymes

Veena

Shanthi

Sachdanandam

2006

Biological & Pharmaceutical Bulletin

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Cancer accounts for high morbidity and high mortality rate throughout the world. Cancer of breast is common in women in developed countries and more than 40% of all breast cancer cases are found in developing countries.1) Cancer is based on genetic and epigenetic alterations that affect the regulation and function of genes. These pathologic changes are selected during progression to yield cells that multiply aggressively beyond the limits normally set by their intrinsic proliferative capacity, neighboring cells, limited mitogens, and blood supply. It is expected that the cataloging of specific changes in gene expression will provide diagnostic markers for any given type. Furthermore, proteins encoded by specific genes could potentially serve as targets for therapeutic intervention. 2)In addition to the therapeutic strategies already known, some recent reports indicate new areas for the development of target selective drugs for the treatment of metastatic breast cancer.3) The earliest adjuvant chemotherapy, with singleagent alkylating and anti-metabolic drugs, was soon replaced by combination therapy, as it was demonstrated that simultaneous combination of two or more agents provided better results. 4,5) Kalpaamruthaa is a Siddha formulation, which has been formulated in our laboratory. They contain Semecarpus anacardium LINN. (SA), Emblica officinalis and honey with different ratio. Kalpaamruthaa (KA) is a Siddha preparation which has been formulated in our laboratory, the components studies of this drug reveals the presence of flavonoids, ascorbic acid, polyphenols, tannins, sugars and sterols. Flavonoids are seemingly more widely dispersed in the food supply and are of more varied composition than are isoflavones. For these reason, their absorption and metabolism have been less completely characterized. Cameron and Pauling 6) believed that ascorbic acid combats cancer by promoting collagen synthesis and thus prevents tumors from invading other tissues. Flavonoids are polyphenols widely distributed and known to possess biological and pharmacological activities, including anti-inflammatory action against free radicals. 7)Isoflavonoids are members of the broad class of plant polyphenols that have been shown in vivo to have benefit in the treatment of a wide variety of chronic diseases, including cancer. Another important consideration in vivo is the localized metabolism of isoflavones that may occur in the vicinity of tumor cells. Many experiments to verify the association between isoflavone intake and disease prevention have been reported. 8)The biochemical evaluation of glycoprotein components, marker enzymes and lysosomal enzymes of liver, kidney and blood of control and experimental rats was carried out to study the therapeutic efficacy of KA on 7,12-dimethylbenz-(a)anthracene (DMBA) induced mammary carcinoma bearing rats (Sprague-Dawley strain). This study was undertaken to arrive at the effective dose of KA in mammary carcinoma condition in rats. MATERIALS AND METHODS MaterialsMammary carcinoma was induced ...

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Section: Discussionmentioning

confidence: 99%

Anticancer Effect of Kalpaamruthaa on Mammary Carcinoma in Rats with Reference to Glycoprotein Components, Lysosomal and Marker Enzymes

Veena

Shanthi

Sachdanandam

2006

Biological & Pharmaceutical Bulletin

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Novel Biochemical Pathways for 5-Fluorouracil in Managing Experimental Hepatocellular Carcinoma in Rats

Abdel-Hamid

Morsy

2010

J Membrane Biol

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Five fluorouracil (5-FU) is extensively used in the treatment of hepatocellular carcinoma (HCC). It is well documented that 5-FU and its metabolites inhibit DNA synthesis through inhibition of thymidylate synthetase. Little is known about additional pathways for 5-FU in managing HCC. The present experiment was mainly designed to study possible biochemical pathways that can be added to 5-FU's mechanisms of action. Four groups of rats constituted a control group (given saline only), a trichloroacetic acid group (TCA, 0.5 g/kg/day for 5 days, orally), a 5-FU-positive group (75 mg/kg body weight, intraperitoneally, once weekly for 3 weeks) and a TCA-treated with 5-FU group (24 h from last TCA dose). We executed both biochemical-serum alpha-fetoprotein (AFP), liver tissue contents of total glycosaminoglycan (TGAGs), collagen (represented as hydroxyproline), total sialic acid (TSA), free glucosamine (FGA) and proteolytic enzyme activity (as pepsin and free cathepsin-D-and histological examinations of the liver tissue. The results revealed histological changes such as central vein congestion and irregularly shaped, substantially enlarged, vesiculated and binucleated hepatocytes. The nuclei were mostly polymorphic and hyperchromatic, and several vacuolation was noticed in the cytoplasm encircling the nucleus with masses of acidophilic material. 5-FU greatly corrected these changes, except that some necrotic and cytotoxic effects of 5-FU were still shown. AFP was significantly elevated in TCA-intoxicated, but reversed in 5-FU-treated, groups. Increased proteolytic activity by TCA was reversed by 5-FU, which also restored TGAG contents to normal; but both TCA and 5-FU depleted collagen content. TCA significantly elevated FGA but depressed TSA; this action was reversed by 5-FU treatment. In conclusion, it is possible that proteolytic activity, expressed as upregulated pepsin and free cathepsin-D activities, is increased in HCC. This is accompanied by extracellular matrix macromolecular disturbance, manifested as decreased TGAGs, collagen and TSA, with marked increase in FGA liver tissue content. The elevated FGA with depressed TSA content of liver tissue may be attributed to a cancer-hampered N-acetylation of FGA into SA. 5-FU administration markedly depressed hepatic tissue proteolysis, possibly reactivated N-acetylation of FGA into SA and elevated TGAGs without stopping tissue fibrosis as collagen was not affected. This study explores additional pathways for the mechanism of action of 5-FU, through conservation of extracellular matrix composition in situ, inhibiting invasion and metastasis in addition to its DNA-disturbing pathway.

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Restoration of energy metabolism in leukemic mice treated by a siddha drug—Semecarpus anacardium Linn. nut milk extract

Dhanasekaran

Shanthi

Sachdanandam

2008

Chemico-Biological Interactions

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Stabilization of lysosomal membrane and cell membrane glycoprotein profile bySemecarpus anacardium Linn. nut milk extract in experimental hepatocellular carcinoma

Cited by 9 publications

References 23 publications

Anticancer Effect of Kalpaamruthaa on Mammary Carcinoma in Rats with Reference to Glycoprotein Components, Lysosomal and Marker Enzymes

Anticancer Effect of Kalpaamruthaa on Mammary Carcinoma in Rats with Reference to Glycoprotein Components, Lysosomal and Marker Enzymes

Novel Biochemical Pathways for 5-Fluorouracil in Managing Experimental Hepatocellular Carcinoma in Rats

Restoration of energy metabolism in leukemic mice treated by a siddha drug—Semecarpus anacardium Linn. nut milk extract

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