Stamen Structure and Function

Scott, Rod; Spielman, Melissa; Dickinson, H. G.

doi:10.1105/tpc.017012

Cited by 581 publications

(576 citation statements)

References 148 publications

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“…We found that this mutation aVects one of the intron splicing recognition sites of the gene, giving rise to an aberrant mRNA, lacking one of the exons. Microscopic observation of the ps-2 phenotype revealed similarities with what was already found in Arabidopsis and tobacco mutants (reviewed by Scott et al 2004;Rieu et al 2003). This new PG gene, is also expressed in maturing fruits, which opens the way to speculation on the possible role of Ps-2 in fruit maturation.…”

Section: Introductionsupporting

confidence: 64%

“…Several mutants aVecting diverse steps in the synthesis of JA in the anthers were identiWed in Arabidopsis. These mutants displayed a delay of anther dehiscence (reviewed by Scott et al 2004). The role of ethylene signaling in this phenomenon was highlighted by Rieu et al (2003) who observed a delay in the dehiscence of anthers of ethylene insensitive tobacco plants.…”

Section: Introductionmentioning

confidence: 98%

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ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene

et al. 2009

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The recessive mutation ps-2, which appeared spontaneously in tomato, confers functional male sterility due to non-dehiscent anthers. In this study, we isolated and characterized the PS-2 gene. A single nucleotide mutation in a novel tomato polygalacturonase gene is responsible for the ps-2 phenotype. The mutation in ps-2 is responsible for an alternative splicing during maturation of the pre-mRNA, which leads to an aberrant mRNA. DiVerentiation between ps-2 and wild type (PS-2) anthers only appears in the Wnal developmental stage in which the stomium remains closed in the mutant. To our knowledge, this is the Wrst functional sterility gene isolated in the Solanaceae family. The speciWc expression of the Arabidopsis homolog of PS-2 in the anther dehiscence zone suggests a conserved mode of action over the plant kingdom, which means that the repression of PS-2 homologs may be a potential way to introduce functional sterility in other species.

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Section: Introductionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 98%

ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene

et al. 2009

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“…Mutations of genes involved in the biosynthesis and signaling of these phytohormones affect stamen filament elongation, anther dehiscence, and pollen viability. However, the molecular mechanisms underlying these effects are unknown (Scott et al, 2004;Wilson et al, 2011). AP-2 may play a role in clathrin-mediated endocytosis for the regulation of phytohormone signaling during floral organ development.…”

Section: Ap-2 Plays Roles In Floral Organ Developmentmentioning

confidence: 99%

“…This appears to be the major cause of the observed reduction in fertility. The maturation of stamens involves cell elongation of the stamen filament, which synchronizes with development of the other floral organs to maximize the fertilization efficiency (Scott et al, 2004;Wilson et al, 2011). It is possible that AP-2 plays a role in filament cell elongation that requires cell wall biogenesis.…”

Section: Ap-2 Plays Roles In Floral Organ Developmentmentioning

confidence: 99%

Identification and Dynamics of Arabidopsis Adaptor Protein-2 Complex and Its Involvement in Floral Organ Development

et al. 2013

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ORCID ID: 0000-0003-4154-9967 (S.Y.).The adaptor protein-2 (AP-2) complex is a heterotetramer involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane in animal cells. The homologous genes of AP-2 subunits are present in the genomes of plants; however, their identities and roles in endocytic pathways are not clearly defined in plants. Here, we reveal the molecular composition of the AP-2 complex of Arabidopsis thaliana and its dynamics on the plasma membrane. We identified all of the a-, b-, s-, and m-subunits of the AP-2 complex and detected a weak interaction of the AP-2 complex with clathrin heavy chain. The m-subunit protein fused to green fluorescent protein (AP2M-GFP) was localized to the plasma membrane and to the cytoplasm. Live-cell imaging using a variable-angle epifluorescence microscope revealed that AP2M-GFP transiently forms punctate structures on the plasma membrane. Homozygous ap2m mutant plants exhibited abnormal floral structures, including reduced stamen elongation and delayed anther dehiscence, which led to a failure of pollination and a subsequent reduction of fertility. Our study provides a molecular basis for understanding AP-2-dependent endocytic pathways in plants and their roles in floral organ development and plant reproduction.

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“…At the same time, the stamen filament elongates and the anther enlarges and expands. Finally, the anther enters a dehiscence program that leads to pollen release when the mature flower opens (Scott et al, 1991(Scott et al, , 2004Goldberg et al, 1993).…”

mentioning

confidence: 99%

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

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To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

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Stamen Structure and Function

Cited by 581 publications

References 148 publications

ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene

ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene

Identification and Dynamics of Arabidopsis Adaptor Protein-2 Complex and Its Involvement in Floral Organ Development

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

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