Standardization of Functional Reporter and Antibiotic Resistance Cassettes to Facilitate the Genetic Engineering of Filamentous Fungi

Sureka, Swati; Chakravorty, Arun K.; Holmes, Eric C.; Spassibojko, Olga; Bhatt, Nupur; Wu, Dongliang; Turgeon, B. Gillian

doi:10.1021/sb5000143

Cited by 12 publications

(5 citation statements)

References 20 publications

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“…Bacteria are single celled, whereas most fungi are multicellular; bacteria have three distinct shapes, while fungi have various shapes which lead to mycelium formation; bacteria reproduce sexually, whereas fungi are capable of reproducing both sexually or asexually. All these differences make fungi more durable and resistant against some antibiotics [ 49 ]. Until now, research has tended to focus on antibacterial nanoparticles rather than on nanoparticles against fungi.…”

Section: Nano Approaches For Mycotoxin Risk Eliminationmentioning

confidence: 99%

Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins

et al. 2018

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Mycotoxins are toxic secondary metabolites produced by certain filamentous fungi. The occurrence of mycotoxins in food and feed causes negative health impacts on both humans and animals. Clay binders, yeast cell walls, or antioxidant additives are the most widely used products for mycotoxin elimination to reduce their impact. Although conventional methods are constantly improving, current research trends are looking for innovative solutions. Nanotechnology approaches seem to be a promising, effective, and low-cost way to minimize the health effects of mycotoxins. This review aims to shed light on the critical knowledge gap in mycotoxin elimination by nanotechnology. There are three main strategies: mold inhibition, mycotoxin adsorption, and reducing the toxic effect via nanoparticles. One of the most promising methods is the use of carbon-based nanomaterials. Graphene has been shown to have a huge surface and high binding capacity for mycotoxins. Attention has also been drawn to polymeric nanoparticles; they could substitute adsorbents or enclose any substance, which would improve the health status of the organism. In light of these findings, this review gives new insights into possible future research that might overcome challenges associated with nanotechnology utilization for mycotoxin elimination from agricultural products.

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Section: Nano Approaches For Mycotoxin Risk Eliminationmentioning

confidence: 99%

Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins

et al. 2018

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“…Finally, genes conferring resistance to antibiotics have been widely used as markers for the selection of transformed cells. The most widely used marker system is the antibiotic resistance to hygromycin [ 310 , 311 ], but also other antibiotics markers are used, such as bialaphos/phosphinothricin, geneticin/neomicyn, phleomycin and kanamycin resistance [ 312 , 313 , 314 ].…”

Section: Strain Screening Methodsmentioning

confidence: 99%

Strategies for the Development of Industrial Fungal Producing Strains

Salazar-Cerezo

Vries

Garrigues

2023

JoF

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The use of microorganisms in industry has enabled the (over)production of various compounds (e.g., primary and secondary metabolites, proteins and enzymes) that are relevant for the production of antibiotics, food, beverages, cosmetics, chemicals and biofuels, among others. Industrial strains are commonly obtained by conventional (non-GMO) strain improvement strategies and random screening and selection. However, recombinant DNA technology has made it possible to improve microbial strains by adding, deleting or modifying specific genes. Techniques such as genetic engineering and genome editing are contributing to the development of industrial production strains. Nevertheless, there is still significant room for further strain improvement. In this review, we will focus on classical and recent methods, tools and technologies used for the development of fungal production strains with the potential to be applied at an industrial scale. Additionally, the use of functional genomics, transcriptomics, proteomics and metabolomics together with the implementation of genetic manipulation techniques and expression tools will be discussed.

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“…Bacteria are unicellular, are of three discrete shapes, and reproduce sexually, while most fungi are multicellular, exhibit a wide range of shapes that can lead to mycelium formation, and possess the ability to reproduce both sexually and asexually. These distinctions make fungi more vigorous and resistant to certain antibiotics . Research efforts have predominantly concentrated on antibacterial NPs, with limited attention given to NPs against fungal infections.…”

Section: Aflatoxin Detoxification Approachesmentioning

confidence: 99%

“…These distinctions make fungi more vigorous and resistant to certain antibiotics. 176 Research efforts have predominantly concentrated on antibacterial NPs, with limited attention given to NPs against fungal infections. The most latest advancements in the realm of antifungal NPs were summarized between 2016 and 2017.…”

Section: Aflatoxin Detoxification Approachesmentioning

confidence: 99%

Aflatoxins in Peanut (Arachis hypogaea): Prevalence, Global Health Concern, and Management from an Innovative Nanotechnology Approach: A Mechanistic Repertoire and Future Direction

Sultana,

Malik,

Raja

et al. 2024

ACS Omega

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Arachis hypogaea is the most significant oilseed nutritious legume crop in agricultural trade across the world. It is recognized as a valued crop for its contributions to nourishing food, as a cooking oil, and for meeting the protein needs of people who are unable to afford animal protein. Currently, its production, marketability, and consumption are hindered because of Aspergillus species infection that consequently contaminates the kernels with aflatoxins. Regarding health concerns, humans and animals are affected by acute and chronic aflatoxin toxicity and millions of people are at high risk of chronic levels. Most methods used to store peanuts are traditional and serve effectively for short-term storage. Now the question for long-term storage has been raised, and this promptly finds potential approaches to the issue. It is imperative to reduce the aflatoxin levels in peanuts to a permissible level by introducing detoxifying innovations. Most of the detoxification reports mention physical, chemical, and biological techniques. However, many current approaches are impractical because of time consumption, loss of nutritional quality, or weak detoxifying efficiency. Therefore, it is crucial to investigate practical, economical, and green methods to control Aspergillus flavus that address current global food security problems. Herein, a green and economically revolutionary way is a nanotechnology that has demonstrated its potential to connect farmers to markets, elevate international marketability, improve human and animal health conditions, and enhance food quality and safety by the management of fungal diseases. Due to the antimicrobial potential of nanoparticles, they act as nanofungicides and have an incredible role in the control of aflatoxins. Nanoparticles have ultrasmall sizes and therefore penetrate the fungal body and invade the pathogen machinery, leading to fungal cell death by ROS production, mutation in DNA, disruption of organelles, and membrane leakage. This is the first mechanistic overview that unveils a comprehensive insight into aflatoxin contamination in peanuts, its prevalence, health effects, and management in addition to nanotechnological interventions that serve as a triple defense approach to detoxify aflatoxins. The optimum use of nanofungicides ensures food safety and the development of goals, especially "zero hunger".

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Standardization of Functional Reporter and Antibiotic Resistance Cassettes to Facilitate the Genetic Engineering of Filamentous Fungi

Cited by 12 publications

References 20 publications

Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins

Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins

Strategies for the Development of Industrial Fungal Producing Strains

Aflatoxins in Peanut (Arachis hypogaea): Prevalence, Global Health Concern, and Management from an Innovative Nanotechnology Approach: A Mechanistic Repertoire and Future Direction

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