Standardization of the Leucocyte Migration Test

Maini, R N; Roffe, Lindsay; Magrath, I. T.; Dumonde, D. C.

doi:10.1159/000231048

Cited by 64 publications

(17 citation statements)

References 7 publications

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“…This suspension was aspirated into capillary tubes which were plugged, centri fuged at 2,000 g, cut at cell fluid interfaces2 and mounted in tissue culture chambers filled with suspending medium. These chambers were then incubated either with or Table I without antigens at 37 °C in a 5°/o C 02 incubator for 18-24 h [7], The migration areas were measured planimetrically and migration indices were calculated by the following formula:…”

Section: Methodsmentioning

confidence: 99%

Leukocyte Migration Inhibition in Nickel Dermatitis

Mirza¹,

Perera²,

Maccia³

et al. 1975

Int Arch Allergy Immunol

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Leukocyte migration inhibitory factor assay was employed as an in vitro diagnostic aid in nickel dermatitis, the second most common contact dermatitis in North America. 15 patch test-positive and 5 patch test-negative patients, all giving a past history suggestive of nickel dermatitis, were investigated. Significant inhibition of leukocyte migration in both groups of patients was obtained only with nickel sulfate-albumin conjugate and not with unconjugated nickel sulfate. Specificity of this system was tested by utilizing an unrelated metallic albumin complex, and no inhibition was found. When patch testing is equivocal or contraindicated, this in vitro technique may be a practical alternative.

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Section: Methodsmentioning

confidence: 99%

Leukocyte Migration Inhibition in Nickel Dermatitis

Mirza¹,

Perera²,

Maccia³

et al. 1975

Int Arch Allergy Immunol

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“…In this study it was possible to discriminate with the sealed capillary migration test between a chro mium-allergic and a control group with a chromate concentration of 1.7 x 10-5 M, especially at 2 and 5 h. On the contrary, using the conventional leukocyte mi gration test, Maini et al [5] found a lesser discrimina tion between controls and patients at earlier times than 18-20 h.…”

Section: Discussionmentioning

confidence: 65%

Leukocytes from Patients Allergic to Chromium and Nickel Examined by the Sealed Capillary Migration Technique

Nordlind¹,

Sandberg²

1983

Int Arch Allergy Immunol

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Leukocytes from patients allergic to chromium and nickel were studied by a sealed capillary migration test. The migration indices were determined at 2, 5 and 24 h. The chromium-allergic group could be differentiated from the control group at all investigated times, and especially at 2 and 5 h, by using a potassium dichromate concentration of 1.7 × 10^––5M in the capillaries. The migration indices in the nickel-allergic group could not be used for discrimination from the controls. The highest chromate (1.1 × 10^––4M) and nickel sulfate (3.8 × 10^––4M) concentrations inhibited the migration of leukocytes from both patients and controls, indicating toxic effects.

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“…Spleens were removed aseptically and the leukocyte migration inhibition (LMI) index was determined by the method of Maini et al (1973) with plasma-membrane proteins as antigen and phytohaemagglutinin (PHA) as nonspecific mitogen. The anti-membrane antibody titres of serum samples collected after completion of immunisation (i.e., before challenge) and at the time the animals were killed were determined by a micro-haemagglutination test (Vinayak et al, 1980a).…”

Section: Immunological Investigationsmentioning

confidence: 99%

Immunoprotective behaviour of plasma-membrane-associated antigens of axenic Entamoeba histolytica

Vinayak

Purnima²,

Saxena³

1987

Journal of Medical Microbiology

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Summary.Immunisation of golden hamsters with plasma-membrane-associated antigens of a virulent subline of axenic Entamoeba histolytica strain NIH 200 V, entrapped in multilamellar phosphatidylcholine liposomes (MPL) or Freund's complete adjuvant (FCA), afforded protection against intrahepatic challenge with axenic amoebic trophozoites of the same strain. Amoebic liver abscess developed in 86% and 80% of the animals that received empty liposomes or buffer emulsified in FCA but in none of the animals that received plasma-membrane-antigen vaccines.All the immunised animals had significantly higher levels (p < 0.001) of antibodies to plasma-membrane components and significantly higher levels (p < 0.00 1) of cellular sensitisation. Antibody-dependent macrophage-mediated cytotoxicity against trophozoites was also found to be significantly greater (p < 0-001) in immunised animals.Liposome-entrapped antigens stimulated the immune system of the host as well as, or better than, antigens administered with FCA.

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Standardization of the Leucocyte Migration Test

Cited by 64 publications

References 7 publications

Leukocyte Migration Inhibition in Nickel Dermatitis

Leukocyte Migration Inhibition in Nickel Dermatitis

Leukocytes from Patients Allergic to Chromium and Nickel Examined by the Sealed Capillary Migration Technique

Immunoprotective behaviour of plasma-membrane-associated antigens of axenic Entamoeba histolytica

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