Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city

Abstract

Introduction: Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates.
 Methodology: This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients’ departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR.
 Results: We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively.
 Conclusions: A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.

Molecular analysis of Panton-Valentine Leucocidin (pvl) gene among MRSA and MSSA isolates

“…A study designed in Lahore city stated nearly matched 40.9% results regarding the prevalence of pvl gene among pathogenic samples as our results 48% shown in the diseased population samples (Iqbal et al, 2018). This number tracked along the highest percentage of pvl gene among the pathogenic samples which is a matter of grave concerns for the health management organization in Pakistan.…”

Community Septicemia Due to Meticillin-resistant Staphylococcus Aureus, Carriers of Panton-valentine Toxin in Children in Tropical Environments.

“…This germ acts through the mediation of an enzyme (penicillinase and beta lactamase) which inactivates the antibiotic. The work of some authors [44] con rms a high prevalence of PVL-MRSA strains resistant to antimicrobials and others [45] the absence of PVL-MRSA strains to antimicrobials.…”