Starvation Induces Phenotypic Diversification and Convergent Evolution in Vibrio vulnificus

Chen, Hwajiun; Chen, Chun‐Yao

doi:10.1371/journal.pone.0088658

Cited by 15 publications

(12 citation statements)

References 56 publications

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“…These mutations allow the mutants to effectively scavenge the nutrients released by dead cells ( Zambrano and Kolter, 1996 ). A number of Gram-positive bacteria such as Listeria monocytogenes ( Bruno and Freitag, 2011 ), Staphylococcus aureus, Enterococcus faecalis , and Bacillus globigii ( Finkel et al, 1997 ) and Gram-negative bacteria including Campylobacter, Geobacter, Vibrio, E. coli, Pseudomonas , etc., have been found to enter the GASP state ( Chen and Chen, 2014 ). Gefen et al (2014) coined the term ‘constant activity stationary phase’ (CASP) to describe the phenomenon of constant rate of protein synthesis observed in non-growing bacteria that have undergone over more than 60 h of starvation.…”

Section: Physiology Of the Stationary Phasementioning

confidence: 99%

Molecular Basis of Stationary Phase Survival and Applications

2017

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Stationary phase is the stage when growth ceases but cells remain metabolically active. Several physical and molecular changes take place during this stage that makes them interesting to explore. The characteristic proteins synthesized in the stationary phase are indispensable as they confer viability to the bacteria. Detailed knowledge of these proteins and the genes synthesizing them is required to understand the survival in such nutrient deprived conditions. The promoters, which drive the expression of these genes, are called stationary phase promoters. These promoters exhibit increased activity in the stationary phase and less or no activity in the exponential phase. The vectors constructed based on these promoters are ideal for large-scale protein production due to the absence of any external inducers. A number of recombinant protein production systems have been developed using these promoters. This review describes the stationary phase survival of bacteria, the promoters involved, their importance, regulation, and applications.

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Section: Physiology Of the Stationary Phasementioning

confidence: 99%

Molecular Basis of Stationary Phase Survival and Applications

2017

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“…These studies often reported changes in cell morphology and composition and pointed to a link between these changes and transition to the VBNC phenotype [7,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

et al. 2016

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Previous work demonstrated that physiological, morphological, and gene expression changes as well as the time-dependent entry into the viable but not culturable (VBNC) state are used by Vibrio species to survive and cope with diverse stress conditions including seasonal temperature downshifts and starvation. To learn more about the nature and specific contribution of membrane proteins to cell adaptation and survival, we analyzed variations in the protein composition of cell envelope and related them to morphological and physiological changes that were taking place during the long-term permanence of Vibrio harveyi in seawater microcosm at 4 °C. We found that after 21 days of permanence, nearly all population (ca. 99 %) of V. harveyi acquired the VBNC phenotype. Although the size of V. harveyi cells gradually decreased during the incubation time, we found that this morphological change was not directly related to their entry into the VBNC state. Our proteomic study revealed that the level of membrane proteins playing key roles in cellular transport, maintenance of cell structure, and in bioenergetics processes remained unchanged along starvation at low temperature, thus suggesting that V. harveyi might need these proteins for the long-term survival and/or for the resuscitation process. On a contrary, the level of two proteins, elongation factor Tu (EF-TU) and bacterioferritin, greatly increased reaching the maximal values by the end of the incubation period. We further discuss the above data with respect to the putative roles likely exerted by membrane proteins during transition to and maintaining of the VBNC state.

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“…Other proteins that became undetectable in starved cells were the methyl-accepting chemotaxis protein I and the flagellin FLIC_ECOLI. Chen and Chen [54] demonstrated that under starvation, Vibrio vulnificus populations exhibited reduced motility. Lemke et al [55] and Chandrangsu et al [56] concluded that DksA (protein required for the regulation of certain promoters) and the alarmone ppGpp inhibit expression of the flagellar cascade during cells' entry into the stationary phase or during their starvation.…”

Section: Resultsmentioning

confidence: 99%

Survival of <i>Escherichia coli</i> under Nutrient-Deprived Conditions: Effect on Cell Envelope Subproteome

Orruño¹,

Parada²,

Kaberdin³

et al. 2017

≪i>Escherichia Coli

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0

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In the aquatic ecosystems, microorganisms are exposed to seasonal and circadian cycles. Abiotic factors (e.g. low temperature, nutrient deprivation) can cause morphological and physiological changes in bacteria, thereby facilitating cell survival. While representing the interface between the cells and external environment, the cell envelope plays a major role in bacterial response to stress and characterization of the changes it undergoes can help to understand the adaptation process. In this study, analysis of the morphological and physiological changes as well as variations in protein composition of the Escherichia coli cell envelope was carried out for populations maintained for 21 days under nutrient deprivation and suboptimal temperatures (4°C and 20°C). It was found that the absence of nutrients led to a temperature-dependent reduction of cell culturability but had no effect on cell viability and integrity. The concentration of membrane proteins playing the key roles in cellular transport, maintenance of cell structure or bioenergetics processes remained mainly unchanged. In contrast, the level of several proteins such as the elongation factor EFTu 1, components of Bam complex or proteins implicated in chemotaxis was altered, thus indicating that cells were readily responding and adapting to stress.

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Starvation Induces Phenotypic Diversification and Convergent Evolution in Vibrio vulnificus

Cited by 15 publications

References 56 publications

Molecular Basis of Stationary Phase Survival and Applications

Molecular Basis of Stationary Phase Survival and Applications

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

Survival of <i>Escherichia coli</i> under Nutrient-Deprived Conditions: Effect on Cell Envelope Subproteome

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