Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

Abstract

This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-L-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P < 0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.

The effect of low static magnetic field on osteogenic and adipogenic differentiation potential of human adipose stromal/stem cells

“…Huang et al [49] reported an increased expression of Col-I in osteoblasts cultured under 0.4 T SMF whilst Feng et al have shown increased ALP activity in cells cultured under a 0.4 T SMF. Although the effect of SMF exposition increases expression of genes responsible for osteogenesis (and this has been proven on osteoblasts cells) its effect on stem cells has not yet been fully determined [50,51].…”

Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

“…Recently, magnetism has been found to positively impact cellular responses [9–11]. Plenty of magnetic composite materials that integrate with magnetic nanoparticles (MNPs) have been manufactured to accelerate the repair and regeneration of engineering tissues [10,12–17].…”

“…For instance, calcium phosphate cements macerated with ultrafine MNPs have significant promotional effects on cell adhesion, proliferation and differentiation [15]. Additionally, biopolymer nanocomposite scaffolds mixed with MNPs increased the adhesion and differentiation of osteoblastic cells in vitro and bone formation in vivo [9,12,14]. From the above examples, MNPs can be considered a valid stimulus to bone fracture healing and new bone formation.…”

Osteogenic differentiation of MC3T3-E1 cells on poly(l-lactide)/Fe3O4 nanofibers with static magnetic field exposure

“…Moderate-intensity SMFs (1 mT-1 T) have been identified capable of affecting a number of biological behaviors of osteoblastic cells [16,17], and it is particularly interesting to researchers because it is easily achieved with permanent magnets. However, the effect of SMF on osteogenic differentiation of osteoblastic cells on magnetic nanofibrous scaffolds of different magnetic properties remains unclear.…”