Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells

Sarvestani, Amir Sabet; Abdolmaleki, Parviz; Mowla, Seyed Javad; Ghanati, Faezeh; Heshmati, Emran; Tavasoli, Zeinab; Jahromi, Azadeh Manoochehri

doi:10.1016/j.micron.2009.10.007

Cited by 38 publications

(33 citation statements)

References 22 publications

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“…This mechanism could occur in our experimental conditions; in fact, at 4 h SMF exposure the transient increase in ROS production observed is also associated to an increased cell growth rate. Moreover, the information available in literature demonstrates that the MF alone does not induce cell cycle arrest [Ding et al, 2001;Tian et al, 2002;Lange et al, 2004;Sarvestani et al, 2009].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

Polidori

Zeppa

Potenza

et al. 2011

Bioelectromagnetics

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In a previous investigation we reported that exposure to a moderate (300 mT) static magnetic field (SMF) causes transient DNA damage and promotes mitochondrial biogenesis in human umbilical vein endothelial cells (HUVECs). To better understand the response of HUVECs to the 300 mT SMF, a high-quality subtracted cDNA library representative of genes induced in cells after 4 h of static magnetic exposure was constructed. The global gene expression profile showed that several genes were induced after the SMF exposure. The characterized clones are involved in cell metabolism, energy, cell growth/division, transcription, protein synthesis, destination and storage, membrane injury, DNA damage/repair, and oxidative stress response. Quantitative real-time polymerase chain reaction (qRT-PCR) experiments were performed at 4 and 24 h on four selected genes. Their expression profiles suggest that HUVEC's response to SMF exposure is transient. Furthermore, compared to control cells, an up-regulation of several genes involved in cell growth and division was observed. This up-regulation is likely to be the cause of the slight, but significant, increase in cell proliferation at 12 h post-treatment. These results provide additional support to the notion that SMFs may be harmless to human health, and could support the rationale for their possible use in medical treatments.

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Section: Discussionmentioning

confidence: 99%

Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

Polidori

Zeppa

Potenza

et al. 2011

Bioelectromagnetics

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“…In fact, its combination with ionized radiation came from soils with high natural radioactivity enhances the indirect mechanisms through increasing the activity, concentration, and life time of free radicals. It can also trigger different cellular processes such as modulating certain enzyme activities, enhancing cellular proliferation, perturbation of the cell cycle (Abdolmaleki et al 2007, Sahebjamei et al 2006, Sarvestani et al 2010). In conclusion, we practiced a simple and efficient technique (chromosome aberration) to detect the genotoxic effects, and we found that the using SMF in conjunction with ionized radiation comes from high-level natural radiation soils enhanced the rate of chromosome aberrations in vicia faba.…”

Section: Discussionmentioning

confidence: 99%

Study the effect of static magnetic field on chromosomal aberrations on Vicia faba in area with high natural radioactivity

2011

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This investigation was performed to evaluate the influence of the static magnetic field (SMF) on chromosomes in Vicia faba cultivated in soil from high background natural activity. Fourteen soil samples were collected from Ramsar area, in north of Iran, which the annual radiation absorbed dose from background radiation is substantially higher than the 20 mSv/year. The specific activity of the radionuclides of 232 Th, 236 Ra, and 40 K was measured using gamma spectrometry. The collected soil samples were then divided into 2 separate groups including HR (high natural radioactivity) and LR (low natural radioactivity). The cultivated Vicia faba in both groups were continuously exposed to SMF of 15 mT for 8 days, each 8 h/day starting from 2nd day. The chromosomal aberrations were counted in both groups with and without SMF from root of samples using chromosomal aberration method. The results showed a significant difference between treated and control plants. Chromosomal aberrations including bridge, fragments, and lagging chromosomes in prophase, metaphase, anaphase, and telophase enhanced significantly in treated groups compared with controls.

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“…tarafından yapılmış bir çalışmada ise statik manyetik alan etkisinin sıçan mezenkimal kök hücrelerinde DNA hasar yanıtı olan G2/M fazındaki hücre sayısını artırdığı gö-rülmüştür. 84 Ueyama ve ark. tarafından, insan kemik iliği mezenkimal kök hücrelerinde yapılan bir çalış-mada, kültür koşullarındaki oksijen miktarının hücre stabilitesinde önemli olduğu, hipoksik koşulların (%5 oksijen) normoksik koşullara göre (%20 oksijen) hücre çoğalmasını hızlandırdığı ve erken dönem pasajlarında kromozomal anormalliklerin ortaya çıkmasına sebep olduğu belirlenmiştir.…”

Section: Mezenki̇mal Kök Hücrelerdeki̇ Genotoksi̇si̇te çAlişmalariunclassified

Bone Marrow Stem Cells and Genotoxicity Studies in These Cells: Review

Çal¹,

Bucurgat²

2017

Turkiye Klinikleri J Pharm Sci

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Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells

Cited by 38 publications

References 22 publications

Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field

Study the effect of static magnetic field on chromosomal aberrations on Vicia faba in area with high natural radioactivity

Bone Marrow Stem Cells and Genotoxicity Studies in These Cells: Review

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