Static Magnetic Fields Promote Osteoblast-Like Cells Differentiation Via Increasing the Membrane Rigidity

Abstract

The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Membrane fluidity was used to evaluate alterations in the biophysical properties of the cellular membranes after the SMF simulation. Our results show that SMF exposure increases alkaline phosphatase activity and extracellular matrix release in MG63 cells. On the other hand, MG63 cells exposed to a 0.4-T SMF exhibited a significant increase in fluorescence anisotropy at 6 h, with a significant reduction in the proliferation effects of growth factors noted at 24 h. Based on these findings, the authors suggest that one of the possible mechanisms that SMF affects osteoblastic maturation is by increasing the membrane rigidity and reducing the proliferation-promoting effects of growth factors at the membrane domain.

Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

“…3, we also found that the proliferation rate of MG63 cells was inhibited by SMF exposure. These results confirmed previous findings that both the continuous SMF-and PEMF-stimulated osteoblasts expressed more ALP activity than control cultures [5,11,17].…”

“…In this study, measurement of the contact angle of the injection-molded PLLA revealed no differences between the SMF-exposed and sham-exposed cell-free PLLA samples, suggesting the changes in cell behavior of MG63 cells on the PLLA substrates in this study (Figs. 3,4,5) were decoupled from the surface characteristics of the PLLA.…”

“…Recently, cell culture studies have demonstrated that, like PEMFs, static magnetic fields (SMF) can induce osteoblastic cell differentiation in the early maturation stage [5,11,23,30]. During SMF stimulation, the cellular membrane is assumed to be the target.…”

Development and biocompatibility tests of electrospun poly-l-lactide nanofibrous membranes incorporating oleic acid-coated Fe3O4

“…It was reported that cell adhesion onto scaffolds is an important step in tissue engineering [8,25]. Since previous studies have found that SMF have a positive effect on cellular proliferation and differentiation [9][10][11][12], it is not surprising that cells cultured on magnetized membranes demonstrate a more spreading morphology. Similar results can be found in several previous studies, which reported that magnetic nanoparticles exhibited a strongly osteoinductive effect [2,8,20].…”

“…For physical factors, static magnetic fields (SMF) have been reported as an inducer of osteoblastic differentiation [9][10][11][12]. It has also been reported that SMF had a significant effect on cell attachment and proliferation on to the surface of scaffold [10].…”