Statistical Correlation between Ligninolytic Enzymes Secretion and Remazol Brilliant Yellow‐3GL Dye Degradation Potential of Trametes versicolor IBL‐04

Abstract

Trametes versicolor IBL-04 was used for biodegradation of Remazol Brilliant Yellow 3-GL (RBY3-GL) reactive textile dye in Kirk's basal salts medium. During the initial screening study, the maximum decolorization (93.5%) of RBY3-GL was achieved in 7 days' shaking incubation period at pH 4 and 30 °C. Different physical and nutritional factors were statistically optimized to enhance the efficiency of T. versicolor IBL-04 for maximum decolorization. Under optimal conditions T. versicolor IBL-04 completely decolorized (100%) the RBY3-GL in 2 days of incubation with negligible adsorption on fungal mycelia. Laccase was the major enzyme (938.3 U/mL) secreted by T. versicolor IBL-04 along with comparatively lower activities of MnP. In this article and for the first time, a statistical correlation has been successfully investigated between the ligninolytic enzymes from an indigenously isolated white rot fungi, T. versicolor IBL-04, and the degradation of RBY3-GL.

Discoloration of dyes by Trametes spp

“…All groups showed values of F (26.60) greater than the values, indicating that there is a signi cant difference in all experiments performed in the present work. Species of Trametes are well studied for discoloration of various synthetic dyes: T. trogii discolored 7% of the remazol brilliant blue (Zouari- Mechichi et al 2006), 8% of indigo carmine (Grassi et al 2011) and 69% of Janus Green and 6% of Poly R-478 (Levin et al 2010); T. hirsuta, 94% indigo carmine, 85% of Bromophenol Blue, 41% of Methyl Orange and 47% Poly R-478 (Rodríguez- Couto et al 2006); T. membranacea, 99.2% of bromophenol blue and 71.8% of methylene blue (Lyra et al 2009); T. pubescens, 59% of Bemaplex Navy M-T and 50% of Bezaktiv Blue BA (Rodríguez-Couto et al 2014); T. versicolor, 93.5% of Remazol Brilliant Yellow 3-GL (Asgher et al 2016); and T. ljubarskyi, 97.7% of reactive violet 5 (Goh et al 2017); T. villosa, 93.8% of acid orange 142 (Ortiz-Monsalve et al 2019); T. polyzona, 90% at 100 mg L-1, 91% at 150mg L-1 and 93% at 200 mg L-1 of indigo carmine (and Uribe-Arizmendi et al 2020). However, T. lactinea has not been tested before for discoloration of dyes, while T. villosa has not been tested for discoloration of indigo carmine.Also, studies of discoloration of this dye using species, not only of Trametes, collected in Brazil are scarce.…”

Microbial manganese peroxidase: a ligninolytic enzyme and its ample opportunities in research

“…In addition, recently the crude MnP enzyme extracted from I. lacteus F17 used for the degradation and detoxification of malachite green dye, which is reported as carcinogenic and mutagenic in nature [38]. In addition, various authors have reported that ligninolytic enzyme has great potential in many biotechnological applications, but unfortunately its lower catalytic efficiencies and its working stabilities limit their practical and versatile applications in many areas of the current industrial use [127][128][129][130][131][132][133][134][135].…”

Biotransformation and detoxification of reactive black dye by Ganoderma tsugae

“…Biological treatment methods are eco-friendly, have been proven to be efficient and more cost-effective and hence are gaining importance in today's situation. Microorganisms such as actinomycetes, fungi, algae, yeast, aerobic and anaerobic bacteria and their enzymes have been successfully utilized to degrade a wide variety of dyes (Kaushik and Malik, 2009;Gupta et al, 2010;Srinivasan et al, 2014;Asgher et al, 2016). But the use of bacteria in the biological treatment of wastewater may result in the generation of colorless, dead-end aromatic amine which is generally more hazardous than the parent compounds and thus may have poor usage and limited application in the treatment of dye effluents (Guaratini et al, 2001;Hadibarata et al, 2013).…”