Status of the endogenous avian leukosis virus in resistant cells from a producing line

Smith, E. A.; Crittenden, L. B.; Brinsfield, T. H.

doi:10.1016/0042-6822(74)90293-1

Cited by 32 publications

(10 citation statements)

References 5 publications

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“…V+C/BE line 100 cells spontaneously produced low titers of RAV-0 and, since they were resistant to exogenous RAV-0 infection due to the absence of receptors required for RAV-0 penetration, presumably contained only endogenous RAV-0-related genetic information (6,8,33). V+C/O line 100 ceUs produced high titers of RAV-0, presumably due to exogenous infection with RAV-0 as a result of exposure to the RAV-0 that they spontaneously produced (6,8,33). Ringneck pheasant ceUs, turkey ceUs, Japanese quail cells, and Pekin duck cels were virus negative (assayed by sedimentable DNA polymerase activity in culture fluids), helper factor negative, and ALV gs antigen negative.…”

Section: Methodsmentioning

confidence: 99%

“…Although the endogenous RAV-0 DNA of uninfected V+C/BE chicken cells was not infectious in transfection assays, the cells spontaneously produced a low titer of RAV-0 and therefore appeared to contain a complete endogenous RAV-0 genome, including a biologically active ALV pol gene (6,8,33). Therefore, the DNA fragments of V+C/BE cells active in marker rescue may have been derived from the pol gene of the endogenous RAV-0 genome.…”

Section: Rsvmentioning

confidence: 99%

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Marker rescue of endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase

Cooper¹

1978

J Virol

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Endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase was studied, using a marker rescue assay to detect biological activity of subgenomic fragments of virus-related DNAs of uninfected avian celLs. Recipient cultures of chicken embryo fibroblasts were treated with sonicated DNA fragments and were infected with a temperature-sensitive mutant of Rous sarcoma virus that encoded a thermolabile DNA polymerase. Wild-type progeny viruses were isolated by marker rescue with fragments of DNA of uninfected chicken, pheasant, quail, and turkey cells. The DNAs of these uninfected avian cells, therefore, appeared to contain endogenous genetic information related to the avian leukosis virus DNA polymerase gene.

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Section: Methodsmentioning

confidence: 99%

Section: Rsvmentioning

confidence: 99%

Marker rescue of endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase

Cooper¹

1978

J Virol

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“…These viruses, of which Rous-associated virus 0 (RAV-0) is the prototype, are classified as endogenous avian retroviruses (2,5,6,27,30). Since these loci are normally expressed at low levels (4, 14,22,28,31), endogenous virus can be isolated from cultured embryonic fibroblasts derived from several inbred lines of chickens. The replication of the endogenous virus in chicken cells is influenced by at least three host functions.…”

mentioning

confidence: 99%

“…First, a majority of chickens do not possess the receptor specific for the subgroup E envelope glycoprotein of the virus. Therefore, exposing fibroblasts from these chickens to the endogenous virus does not result in viral attachment and penetration (7,28,30). Second, chickens that do express the appropriate receptor frequently coexpress a glycoprotein which binds specifically to the receptor, thus preventing attachment of the endogenous virus (7,10,20,23,32).…”

mentioning

confidence: 99%

Replication of endogenous avian retrovirus in permissive and nonpermissive chicken embryo fibroblasts

Humphries¹,

Allen²

1984

J Virol

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Clones of chicken embryo fibroblasts exogenously infected with the endogenous avian retrovirus were analyzed to examine the replication of this virus in permissive (Gr+) and nonpermissive (Gr-) cells. The results demonstrate that the endogenous virus was capable of infecting both Gr+ and Grcells with equal efficiency. Infected clones of Gr+ and Gr-cells differed, however, in two significant ways. At the time of their initial characterization, the Gr+ clones produced 100to 1,000-fold more virus than the Grclones. Further, the amount of virus produced by Gr+ clones did not change significantly during serial passage of the cells. In contrast, continued passage of the infected Gr-clones resulted in a gradual increase in the amount of virus produced. Individual clones of infected Gr-cells produced infectious virus at rates that, initially, differed by a factor of more than 104. The large differences in the production of virus by these

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“…Endogenous viruses are expressed at various levels in different chicken lines and signal factors governing the level of their expression include the ability of the cells to be infected with viruses of subgroup E (Smith et al, 1974) and the ability of these cells to support replication of endogenous viruses (Robinson, 1976;Linial & Neiman, 1976), Chickens are characterized for the expression of endogenous antigens and endogenous virus by examining either feather follicle cells or embryos obtained from trap-nested hens (Robinson & Lamoreux, 1976;Crittenden et al, 1979b).…”

Section: Introductionmentioning

confidence: 99%

Variation in Susceptibility to Avian Sarcoma Viruses and Expression of Endogenous Avian Leukosis Virus Antigens in Specific Pathogen-free Chicken Lines

Ignjatovic

Bagust

1985

Journal of General Virology

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SUMMARYFive lines of chickens maintained as specific pathogen-free flocks in Australia were characterized in relation to endogenous antigens and endogenous avian leukosis virus expression. Embryos of line N were predominantly of C/E phenotype, uniformly positive for group-specific antigen and chick helper factor (gs+chf +) and 38~ expressed endogenous virus at a very low titre. Embryos of line M4 were uniformly of C/ABE phenotype and were either gs+chf + or gs-chf +. Line W19 embryos segregated for susceptibility to viruses of subgroup A, B and D and were either of C/E or C/ABE phenotype. The majority of W 19 embryos were gs+chf + with a small proportion being gs+chf -. Line I 13 embryos were either of C/0 or C/ABE phenotype, uniformly gs-chfand 44~ of embryos expressed endogenous virus at a low titre. Line S segregated for susceptibility to subgroup E virus and embryos were either of C/E or C/0 phenotype, while the majority of embryos from line S were gs-chf-with some embryos being gs+chf + or gs-chf +. The degree of interference of gs+chf + and gs-chf ÷ phenotypes with subgroup E virus infection was identical with the interference patterns of classical gs+chf + and gs-chf + phenotypes. The resistance to infection with avian sarcoma viruses of subgroups E in lines N and M4, and to a degree in line W19, was highly associated with the presence of chf. Resistance to subgroup E virus was independent of chf in lines S and I13, probably being under the control of an independent locus. Cellular restriction of endogenous virus replication existed in all subgroup E virussusceptible cells of line I 13 in contrast to cells of line S which supported replication of endogenous virus. The phenotype of chicken cells for the expression of endogenous gs antigen and chf could accurately be predicted from the test performed on whole blood cells.

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Status of the endogenous avian leukosis virus in resistant cells from a producing line

Cited by 32 publications

References 5 publications

Marker rescue of endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase

Marker rescue of endogenous cellular genetic information related to the avian leukosis virus gene encoding RNA-directed DNA polymerase

Replication of endogenous avian retrovirus in permissive and nonpermissive chicken embryo fibroblasts

Variation in Susceptibility to Avian Sarcoma Viruses and Expression of Endogenous Avian Leukosis Virus Antigens in Specific Pathogen-free Chicken Lines

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