Status of the tandem accelerator mass spectrometry facility at Peking University

Chen, Chia-erh; Guo, Zhiyu; Yan, Shengqing; Zhang, Zhengfang; Gong, Lei; Renxing, Li; Yu, Jin; Li, Kun; Liu, Hongtao; Zhang, Ruju; Liu, Kexin; Tiemei, Chen; Yuan, Shijun; Si, H.Z.; Weizhong, Zhang

doi:10.1016/0168-583x(90)90427-v

Cited by 20 publications

(2 citation statements)

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“…The 14C contents were measured by a protocol developed for the 2x6 MV EN Tandem AMS facility at the Institute of Heavy Ion Physics, Peking University. Details on this procedure can be found elsewhere (Chen et al 1990). Our AMS allows measurements of the isotope ratio 14C/12C with a sensitivity of 7x10'15 and an instrument precision of 1%-4%.…”

Section: Methodsmentioning

confidence: 99%

Genotoxicity Study on Nicotine and Nicotine-Derived Nitrosamine by Accelerator Mass Spectrometry

Wang

Shi

et al. 1996

Radiocarbon

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We have studied DNA adduction with 14C-labeled nicotine and nicotine-derived nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), by accelerator mass spectrometry (AMS) in mouse liver at doses equivalent to low-level exposure of humans. The dose ranges of nicotine and NNK administered were from 0.4 μg to 4.0×102 μg kg b.w.-1, and from 0.1 μg to 2.0×104 μg kg b.w.-1, respectively. In the exposure of mice to either nicotine or NNK, the number of DNA adducts increased linearly with increasing dose. The detection limit of DNA adducts was 1 adduct per 1011 nucleotide molecules. This limit is 1–4 orders of magnitude lower than that of other techniques used for quantification of DNA adducts. The results of our animal experiments enabled us to speculate that nicotine is a potential carcinogen. According to the procedure for 14C-labeled-NNK synthesis, we discuss the ultimate chemical speciation of NNK bound to DNA. From the animal tests we derived a directly perceivable relation between tobacco consumption and DNA adduction as the carcinogenic risk assessment.

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Section: Methodsmentioning

confidence: 99%

Genotoxicity Study on Nicotine and Nicotine-Derived Nitrosamine by Accelerator Mass Spectrometry

Wang

Shi

et al. 1996

Radiocarbon

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“…The samples were measured on the 2Â 6 MV EN Tandem AMS facility at the Institute of Heavy Ion Physics, Peking University (Chen et al, 1990), and the results are given as the 14 C/ 12 C ratio with the unit of modern carbon (MC) (1 MC equals to 5.9 Â 10 10 14 C/g of carbon, that is, 97.9 Â 10 À18 mol of 14 C/mg of carbon). This AMS system can measure the 14 C/ 12 C ratio with a sensitivity of 7 Â 10 À15 and an instrument precision of 1%-4%.…”

Section: Ams Sample Preparation and Measurementmentioning

confidence: 99%

Formation of MTBE-DNA adducts in mice measured with accelerator mass spectrometry

Wang

et al. 2005

Environ. Toxicol.

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Methyl tert-butyl ether (MTBE) is a gasoline oxygenate and antiknock additive substituting for lead alkyls currently in use worldwide. Previous studies have shown that MTBE at very high doses induces tumors in rodents. The aim of the present study was to examine directly the binding ability of MTBE onto DNA, demonstrating its potential genotoxicity. MTBE-DNA adducts and their decay kinetics in mice have been measured by using doubly 14C-labeled MTBE with an advanced, ultrasensitive technique: accelerator mass spectrometry (AMS). It was found that MTBE definitely formed adducts with DNA in mouse lung, liver, and kidney in a log/log linear dose-response relationship. The distribution sequence of DNA adducts in these tissues is: lung > liver > kidney. The level of MTBE-DNA adducts peaked at 12 h postadministration in the lung and peaked at 6 h postadministration in the liver. Then the adducts declined rapidly until 5 days postadministration and thereafter declined much more slowly. To our knowledge, this is the first report on DNA adduction with MTBE in vivo. The mechanism of the formation of MTBE-DNA adducts also is discussed.

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Genotoxic effects of low-dose exposure to pirimicarb studied with accelerator mass spectrometry

Wang

et al. 1997

Chin.Sci.Bull.

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Status of the tandem accelerator mass spectrometry facility at Peking University

Cited by 20 publications

References 1 publication

Genotoxicity Study on Nicotine and Nicotine-Derived Nitrosamine by Accelerator Mass Spectrometry

Genotoxicity Study on Nicotine and Nicotine-Derived Nitrosamine by Accelerator Mass Spectrometry

Formation of MTBE-DNA adducts in mice measured with accelerator mass spectrometry

Genotoxic effects of low-dose exposure to pirimicarb studied with accelerator mass spectrometry

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