Stellera chamaejasme and Its Main Compound Luteolin 7-O-Glucoside Alleviates Skin Lesions in Oxazolone- and 2,4-Dinitrochlorobenzene-Stimulated Murine Models of Atopic Dermatitis

Abstract

Stellera chamaejasme, also known as “Langdu”, has been traditionally used for the management of skin-related diseases such as psoriasis and skin ulcers. The aim of this study was to determine whether S. chamaejasme and its major component, luteolin 7-O-glucoside, have a preventive effect on the development of atopic dermatitis in oxazolone-treated BALB/c mice and 2,4-dinitrochlorobenzene-treated hairless mice. The epicutaneous applications of oxazolone and 2,4-dinitrochlorobenzene evoke an experimental murine atopic dermatitis-like reaction in BALB/c mouse ears and SKH-1 hairless mice. Atopic skin symptoms, including erythema (redness), pruritus (itching), exudation (weeping), excoriation (peeling), and lichenification (skin thickening), responded to treatment with S. chamaejasme aerial parts EtOH extract for 2 or 3 weeks. Histopathological examination revealed S. chamaejasme aerial parts EtOH extract significantly reduced inflammatory cell infiltration when applied to atopic dermatitis mice. In addition, luteolin 7-O-glucoside, the major active compound of the S. chamaejasme aerial parts EtOH extract, decreased serum IgE and IL-4 levels and transepidermal water loss and increased skin hydration, therefore exhibiting strong anti-atopic dermatitis activity in 2,4-dinitrochlorobenzene-induced atopic dermatitis mice. In this study, we confirmed antipruritic and antidermatitic effects of S. chamaejasme extract and its main component luteolin 7-O-glucoside in atopic dermatitis murine models. The study shows S. chamaejasme aerial parts EtOH extract and luteolin 7-O-glucoside are most likely to be potential drug candidates for atopic dermatitis treatment.

Luteolin as a modulator of skin aging and inflammation

“…In addition, the major active compound of the EtOH extract, luteolin 7-O-glucoside, decreased serum IgE and IL-4 levels, epidermal thickening as well as transepidermal water loss and increased skin hydration, therefore, showing strong anti-atopic dermatitis activity. 93 In addition, in the proliferative canine keratinocyte cell line CPEK, luteolin down modulated expression of IL-33, IL-1β, IL-6 and IL-8 after lipopolysaccharide (LPS) stimulation, indicating that luteolin might also enable the treatment of canine atopic dermatitis. 94 Interestingly, luteolin as the active component of Perilla frutescens L. (Lamiaceae) was also shown to inhibit the production of IL-4 in a house dust mite (HDM) induced murine Balb/c model of allergic rhinitis.…”

“…Regarding other forms of dermatitis, Jo et al have analyzed the effects of an ethanol extract of the aerial parts of Stellera chamaejasme L. (Thymelaeaceae) on oxazolone-or 2,4-dinitrochlorobenzene stimulated murine models of atopic dermatitis (AD). 93 Stellera chamaejasme contains diverse flavonoids and coumarins with anti-oxidative, anti-viral and anti-cancer activities as well as anti-inflammatory, analgesic and wound healing activities and exerted anti-atopic properties in the AD models. In addition, the major active compound of the EtOH extract, luteolin 7-O-glucoside, decreased serum IgE and IL-4 levels, epidermal thickening as well as transepidermal water loss and increased skin hydration, therefore, showing strong anti-atopic dermatitis activity.…”

Anti-Atopic Effect of Acorn Shell Extract on Atopic Dermatitis-Like Lesions in Mice and Its Active Phytochemicals

“…In addition, the efficacy of dupilumab, the drug that regulates IL-4, is known to be the best in the clinical trial. Previous papers have demonstrated that the extracts of natural products inhibiting IL-4 expression and some of the compounds isolated therefrom have excellent efficacy against atopic dermatitis in vivo [50,51] Therefore, compounds that inhibit IL-4 expression can be inferred to be able to ameliorate atopic dermatitis in vivo. Gallic acid and ellagic acid have previously been reported to have extensive anti-inflammatory activities, as revealed in our experiments.…”

Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis

“…Inflamed cells were treated with the nutraceutical mixture containing luteolin, piceatannol, and cannabidiol at doses established through the literature and MTT assay results (22)(23)(24). The effects of the individual mixture components on the skin inflammation markers were already known in the literature so their synergic ability has been evaluated here (25)(26)(27). Piceatannol [dissolved in dimethyl sulfoxide (DMSO)] and luteolin and cannabidiol (dissolved in methanol) were added directly to the culture medium at the following concentrations (Mix 1: cannabidiol 10 µM, luteolin 10 µM, and piceatannol 10 µM; Mix 2: 10:25:25; Mix 3: 10:50:50; and Mix 4: 10:100:100 µM) for 4 h and 8 h. The culture medium containing vehicles alone was added to controls under the same conditions.…”