Stem cell manipulation, gene therapy and the risk of cancer stem cell emergence

Clément, Flora; Grockowiak, Élodie; Zylbersztejn, Florence; Fossard, Gaëlle; Gobert, Stéphanie; Maguer-Satta, Véronique

doi:10.21037/sci.2017.07.03

Cited by 34 publications

(26 citation statements)

References 131 publications

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“…Strategies for promoting the development of follicles, maintaining normal follicle after chemotherapy or radiotherapy have been important strategies as future clinical therapies for treating infertility. Stem cells are proved to be useful in recover the ovary damage in the chemotherapy POF disease [6,8], [23], but stem cell may also cause recurrence of the tumor risk [24].…”

Section: Discussionmentioning

confidence: 99%

Niacin Inhibits Apoptosis and Rescues Premature Ovarian Failure

Wang¹,

Sun²,

Yu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Over 99% of mouse and human ovarian follicles will undergo specialized cell death including atresia and apoptosis. Reduction of apoptosis may help reduce infertility and maintain the reproductive ability in women. Methods: 3-day B6D2F1 mice were used to culture small follicle and ovary tissue with niacin and 18-day mice were intraperitoneal injected with niacin to determine its effect on follicle development. Then establish 8-weeks POF animal model with cytoxan (CTX) or radiation. Treatment group was given 0.1 mL of 100 mM niacin by an intraperitoneal injection twice before ovulation. The ovaries were collected and the follicles were counted and categorized, and ovarian histologic sections were stained for TUNEL. Ovarian function was then evaluated by monitoring ovulation. Microarray analyses, Western blot, immunofluorescence and real-time quantitative PCR were used to assess the mechanism of ovarian injury and repair. Results: We found that niacin promotes follicle growth in the immature oocyte and it increased the levels of a germ-line cell marker DDX4, and a cell proliferation marker PCNA in the ovary. Addition of niacin to the cell culture reduced oocyte apoptosis in vitro. Administration of niacin to treat premature ovarian failure (POF) in mouse models showed inhibition of follicular apoptosis under harmful conditions, such as radiation and chemotherapy damage, by markedly reducing cumulus cell apoptosis. Additionally, the number of developing follicles increased after administration of niacin. Conclusion: Niacin may have an important function in treating POF by reducing apoptosis in clinical applications.

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Section: Discussionmentioning

confidence: 99%

Niacin Inhibits Apoptosis and Rescues Premature Ovarian Failure

Wang¹,

Sun²,

Yu³

et al. 2018

Cell Physiol Biochem

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“…To overcome this problem, gene transfection to improve the survival and proliferation of the used vascular cells has been suggested [110,112]. However, this genetic manipulation bears an oncogenic risk [113].…”

Section: Stem Cells Internationalmentioning

confidence: 99%

Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

Helder

Bravenboer

et al. 2019

Stem Cells International

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Bone tissue engineering techniques are a promising alternative for the use of autologous bone grafts to reconstruct bone defects in the oral and maxillofacial region. However, for successful bone regeneration, adequate vascularization is a prerequisite. This review presents and discusses the application of stem cells and new strategies to improve vascularization, which may lead to feasible clinical applications. Multiple sources of stem cells have been investigated for bone tissue engineering. The stromal vascular fraction (SVF) of human adipose tissue is considered a promising single source for a heterogeneous population of essential cells with, amongst others, osteogenic and angiogenic potential. Enhanced vascularization of tissue-engineered grafts can be achieved by different mechanisms: vascular ingrowth directed from the surrounding host tissue to the implanted graft, vice versa, or concomitantly. Vascular ingrowth into the implanted graft can be enhanced by (i) optimizing the material properties of scaffolds and (ii) their bioactivation by incorporation of growth factors or cell seeding. Vascular ingrowth directed from the implanted graft towards the host tissue can be achieved by incorporating the graft with either (i) preformed microvascular networks or (ii) microvascular fragments (MF). The latter may have stimulating actions on both vascular ingrowth and outgrowth, since they contain angiogenic stem cells like SVF, as well as vascularized matrix fragments. Both adipose tissue-derived SVF and MF are cell sources with clinical feasibility due to their large quantities that can be harvested and applied in a one-step surgical procedure. During the past years, important advancements of stem cell application and vascularization in bone tissue regeneration have been made. The development of engineered in vitro 3D models mimicking the bone defect environment would facilitate new strategies in bone tissue engineering. Successful clinical application requires innovative future investigations enhancing vascularization.

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“…Gene therapies for the skin have been studied extensively over the years and have met with limited success [ 62 – 64 ]. Risks from use of retrovirus-based expression systems suggest that lentiviral-mediated genetic modifications may have greater safety and efficacy in prospective studies [ 65 , 66 ]. However, at least two examples of gene therapy in skin substitutes are currently active in the areas of innate antimicrobial peptides (e.g., cathelicidins, β-defensins) [ 67 , 68 ] in allogeneic engineered skin to promote healing of chronic wounds and collagen VII for recessive dystrophic epidermolysis bullosa (RDEB) [ 69 , 70 ].…”

Section: Reviewmentioning

confidence: 99%

Tissue engineering of skin and regenerative medicine for wound care

2018

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ᅟEngineering of biologic skin substitutes has progressed over time from individual applications of skin cells, or biopolymer scaffolds, to combinations of cells and scaffolds for treatment, healing, and closure of acute and chronic skin wounds. Skin substitutes may be categorized into three groups: acellular scaffolds, temporary substitutes containing allogeneic skin cells, and permanent substitutes containing autologous skin cells. Combined use of acellular dermal substitutes with permanent skin substitutes containing autologous cells has been shown to provide definitive wound closure in burns involving greater than 90% of the total body surface area. These advances have contributed to reduced morbidity and mortality from both acute and chronic wounds but, to date, have failed to replace all of the structures and functions of the skin. Among the remaining deficiencies in cellular or biologic skin substitutes are hypopigmentation, absence of stable vascular and lymphatic networks, absence of hair follicles, sebaceous and sweat glands, and incomplete innervation. Correction of these deficiencies depends on regulation of biologic pathways of embryonic and fetal development to restore the full anatomy and physiology of uninjured skin. Elucidation and integration of developmental biology into future models of biologic skin substitutes promises to restore complete anatomy and physiology, and further reduce morbidity from skin wounds and scar. This article offers a review of recent advances in skin cell thrapies and discusses the future prospects in cutaneous regeneration.

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Stem cell manipulation, gene therapy and the risk of cancer stem cell emergence

Cited by 34 publications

References 131 publications

Niacin Inhibits Apoptosis and Rescues Premature Ovarian Failure

Niacin Inhibits Apoptosis and Rescues Premature Ovarian Failure

Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

Tissue engineering of skin and regenerative medicine for wound care

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